Cargando…

Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (LcyE) Gene Using the CRSPR-Cas9/HDR and Geminiviral Replicon System in Rice

Lycopene epsilon-cyclase (LcyE) is a key enzyme in the carotenoid biosynthetic pathway of higher plants. Using the CRSPR/Cas9 and the geminiviral replicon, we optimized a method for targeted mutagenesis and golden SNP replacement of the LcyE gene in rice. We have exploited the geminiviral replicon a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kim, Jong Hee, Yu, Jihyeon, Kim, Hee Kyoung, Kim, Jin Young, Kim, Me-Sun, Cho, Yong-Gu, Bae, Sangsu, Kang, Kwon Kyoo, Jung, Yu Jin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499184/ https://www.ncbi.nlm.nih.gov/pubmed/36142294 http://dx.doi.org/10.3390/ijms231810383

Ejemplares similares

Identification of Lycopene epsilon cyclase (LCYE) gene mutants to potentially increase β-carotene content in durum wheat (Triticum turgidum L.ssp. durum) through TILLING
por: Richaud, Daniela, et al.
Publicado: (2018)

Highly efficient homology‐directed repair using CRISPR/Cpf1‐geminiviral replicon in tomato
por: Vu, Tien Van, et al.
Publicado: (2020)

Validation of the effects of molecular marker polymorphisms in LcyE and CrtRB1 on provitamin A concentrations for 26 tropical maize populations
por: Babu, Raman, et al.
Publicado: (2012)

Functional characterization and comparison of lycopene epsilon-cyclase genes in Nicotiana tabacum
por: Song, Weina, et al.
Publicado: (2022)

5′-UTR allelic variants and expression of the lycopene-ɛ-cyclase LCYE gene in maize (Zea mays L.) inbred lines of Russian selection
por: Arkhestova, D.Kh., et al.
Publicado: (2023)

In-planta Gene Targeting in Barley Using Cas9 With and Without Geminiviral Replicons
por: Lawrenson, Tom, et al.
Publicado: (2021)

5′ and 3′ Untranslated Regions Strongly Enhance Performance of Geminiviral Replicons in Nicotiana benthamiana Leaves
por: Diamos, Andrew G., et al.
Publicado: (2016)

Metabolic engineering of potato tuber carotenoids through tuber-specific silencing of lycopene epsilon cyclase
por: Diretto, Gianfranco, et al.
Publicado: (2006)

Is LCI the best for virtual chromoendoscopy?
por: Arantes, Vitor, et al.
Publicado: (2019)

Geminiviral Triggers and Suppressors of Plant Antiviral Immunity
por: Teixeira, Ruan M., et al.
Publicado: (2021)

Characterisation of novel endogenous geminiviral elements in macadamia
por: Zakeel, Mohamed C. M., et al.
Publicado: (2021)

Transcriptome and Metabolite Profiling of Tomato SGR-Knockout Null Lines Using the CRISPR/Cas9 System
por: Kim, Jin Young, et al.
Publicado: (2022)

Generation of an MC3R knock-out pig by CRSPR/Cas9 combined with somatic cell nuclear transfer (SCNT) technology
por: Yin, Yajun, et al.
Publicado: (2019)

Generation and Transcriptome Profiling of Slr1-d7 and Slr1-d8 Mutant Lines with a New Semi-Dominant Dwarf Allele of SLR1 Using the CRISPR/Cas9 System in Rice
por: Jung, Yu Jin, et al.
Publicado: (2020)

Extended Set of GoldenBraid Compatible Vectors for Fast Assembly of Multigenic Constructs and Their Use to Create Geminiviral Expression Vectors
por: Dusek, Jakub, et al.
Publicado: (2020)

Molecular and functional characterization of a novel chromoplast-specific lycopene β-cyclase from Citrus and its relation to lycopene accumulation
por: Alquézar, Berta, et al.
Publicado: (2009)

KnowVolution of the Polymer-Binding Peptide LCI for Improved Polypropylene Binding
por: Rübsam, Kristin, et al.
Publicado: (2018)

Do clinimetric properties of LCI change after correction of signal processing?
por: Frauchiger, Bettina S., et al.
Publicado: (2022)

A Lycopene ε-Cyclase TILLING Allele Enhances Lycopene and Carotenoid Content in Fruit and Improves Drought Stress Tolerance in Tomato Plants
por: Petrozza, Angelo, et al.
Publicado: (2023)

Large-scale survey reveals pervasiveness and potential function of endogenous geminiviral sequences in plants
por: Sharma, Vikas, et al.
Publicado: (2020)

Les 20 ans du web en direct sur LCI.fr
Publicado: (2009)

Effect of the Citrus Lycopene β-Cyclase Transgene on Carotenoid Metabolism in Transgenic Tomato Fruits
por: Guo, Fei, et al.
Publicado: (2012)

The kiwifruit lycopene beta-cyclase plays a significant role in carotenoid accumulation in fruit
por: Ampomah-Dwamena, Charles, et al.
Publicado: (2009)

Neofunctionalization of Chromoplast Specific Lycopene Beta Cyclase Gene (CYC-B) in Tomato Clade
por: Mohan, Vijee, et al.
Publicado: (2016)

Isolation and Functional Characterization of a Lycopene β-cyclase Gene Promoter from Citrus
por: Lu, Suwen, et al.
Publicado: (2016)

Lycopene β-cyclase expression influences plant physiology, development, and metabolism in tobacco plants
por: Kössler, Stella, et al.
Publicado: (2021)

Cloning of the Lycopene β-cyclase Gene in Nicotiana tabacum and Its Overexpression Confers Salt and Drought Tolerance
por: Shi, Yanmei, et al.
Publicado: (2015)

Evaluation of novel LCI CAD EYE system for real time detection of colon polyps
por: Neumann, Helmut, et al.
Publicado: (2021)

Isolation and functional characterization of Lycopene β-cyclase (CYC-B) promoter from Solanum habrochaites
por: Dalal, Monika, et al.
Publicado: (2010)

Functional Analysis of the Phycomyces carRA Gene Encoding the Enzymes Phytoene Synthase and Lycopene Cyclase
por: Sanz, Catalina, et al.
Publicado: (2011)

Levels of Lycopene β-Cyclase 1 Modulate Carotenoid Gene Expression and Accumulation in Daucus carota
por: Moreno, Juan Camilo, et al.
Publicado: (2013)

The lycopene β-cyclase plays a significant role in provitamin A biosynthesis in wheat endosperm
por: Zeng, Jian, et al.
Publicado: (2015)

Enhancement of β-carotene content in Chlamydomonas reinhardtii by expressing bacterium-driven lycopene β-cyclase
por: Huang, Danqiong, et al.
Publicado: (2023)

Anticancer Effect of Lycopene in Gastric Carcinogenesis
por: Kim, Mi Jung, et al.
Publicado: (2015)

Targeted disruption of tomato chromoplast‐specific lycopene β‐cyclase (CYC‐B) gene promotes early accumulation of lycopene in fruits and enhanced postharvest cold tolerance
por: Arruabarrena, Ana, et al.
Publicado: (2023)

Biochemical Characterization of Orange-Colored Rice Calli Induced by Target Mutagenesis of OsOr Gene
por: Kim, Hee Kyoung, et al.
Publicado: (2022)

The genomes of many yam species contain transcriptionally active endogenous geminiviral sequences that may be functionally expressed
por: Filloux, Denis, et al.
Publicado: (2015)

The load carriage index (LCI) - adjusting the load carried by the soldier according to body composition measurements
por: Ketko, Itay, et al.
Publicado: (2015)

SlBEL11 affects tomato carotenoid accumulation by regulating SlLCY-b2
por: He, Yan, et al.
Publicado: (2022)

Cloning and Functional Characterization of a Lycopene β-Cyclase from Macrophytic Red Alga Bangia fuscopurpurea
por: Cao, Tian-Jun, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_8khkhnuprqmhc43j6scbh6hh4c