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Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination

The antituberculosis vaccine Bacillus Calmette–Guérin (BCG) induces nonspecific protection against heterologous infections, at least partly through induction of innate immune memory (trained immunity). The amplitude of the response to BCG is variable, but the factors that influence this response are...

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Autores principales: Koeken, Valerie A. C. M., Qi, Cancan, Mourits, Vera P., de Bree, L. Charlotte J., Moorlag, Simone J. C. F. M., Sonawane, Vidhisha, Lemmers, Heidi, Dijkstra, Helga, Joosten, Leo A. B., van Laarhoven, Arjan, Xu, Cheng-Jian, van Crevel, Reinout, Netea, Mihai G., Li, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499240/
https://www.ncbi.nlm.nih.gov/pubmed/36094960
http://dx.doi.org/10.1371/journal.pbio.3001765
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author Koeken, Valerie A. C. M.
Qi, Cancan
Mourits, Vera P.
de Bree, L. Charlotte J.
Moorlag, Simone J. C. F. M.
Sonawane, Vidhisha
Lemmers, Heidi
Dijkstra, Helga
Joosten, Leo A. B.
van Laarhoven, Arjan
Xu, Cheng-Jian
van Crevel, Reinout
Netea, Mihai G.
Li, Yang
author_facet Koeken, Valerie A. C. M.
Qi, Cancan
Mourits, Vera P.
de Bree, L. Charlotte J.
Moorlag, Simone J. C. F. M.
Sonawane, Vidhisha
Lemmers, Heidi
Dijkstra, Helga
Joosten, Leo A. B.
van Laarhoven, Arjan
Xu, Cheng-Jian
van Crevel, Reinout
Netea, Mihai G.
Li, Yang
author_sort Koeken, Valerie A. C. M.
collection PubMed
description The antituberculosis vaccine Bacillus Calmette–Guérin (BCG) induces nonspecific protection against heterologous infections, at least partly through induction of innate immune memory (trained immunity). The amplitude of the response to BCG is variable, but the factors that influence this response are poorly understood. Metabolites, either released by cells or absorbed from the gut, are known to influence immune responses, but whether they impact BCG responses is not known. We vaccinated 325 healthy individuals with BCG, and collected blood before, 2 weeks and 3 months after vaccination, to assess the influence of circulating metabolites on the immune responses induced by BCG. Circulating metabolite concentrations after BCG vaccination were found to have a more pronounced impact on trained immunity responses, such as the increase in IL-1β and TNF-α production upon Staphylococcus aureus stimulation, than on specific adaptive immune memory, assessed as IFN-γ production in response to Mycobacterium tuberculosis. Circulating metabolites at baseline were able to predict trained immunity responses at 3 months after vaccination and enrichment analysis based on the metabolites positively associated with trained immunity revealed enrichment of the tricarboxylic acid (TCA) cycle and glutamine metabolism, both of which were previously found to be important for trained immunity. Several new metabolic pathways that influence trained immunity were identified, among which taurine metabolism associated with BCG-induced trained immunity, a finding validated in functional experiments. In conclusion, circulating metabolites are important factors influencing BCG-induced trained immunity in humans. Modulation of metabolic pathways may be a novel strategy to improve vaccine and trained immunity responses.
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spelling pubmed-94992402022-09-23 Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination Koeken, Valerie A. C. M. Qi, Cancan Mourits, Vera P. de Bree, L. Charlotte J. Moorlag, Simone J. C. F. M. Sonawane, Vidhisha Lemmers, Heidi Dijkstra, Helga Joosten, Leo A. B. van Laarhoven, Arjan Xu, Cheng-Jian van Crevel, Reinout Netea, Mihai G. Li, Yang PLoS Biol Research Article The antituberculosis vaccine Bacillus Calmette–Guérin (BCG) induces nonspecific protection against heterologous infections, at least partly through induction of innate immune memory (trained immunity). The amplitude of the response to BCG is variable, but the factors that influence this response are poorly understood. Metabolites, either released by cells or absorbed from the gut, are known to influence immune responses, but whether they impact BCG responses is not known. We vaccinated 325 healthy individuals with BCG, and collected blood before, 2 weeks and 3 months after vaccination, to assess the influence of circulating metabolites on the immune responses induced by BCG. Circulating metabolite concentrations after BCG vaccination were found to have a more pronounced impact on trained immunity responses, such as the increase in IL-1β and TNF-α production upon Staphylococcus aureus stimulation, than on specific adaptive immune memory, assessed as IFN-γ production in response to Mycobacterium tuberculosis. Circulating metabolites at baseline were able to predict trained immunity responses at 3 months after vaccination and enrichment analysis based on the metabolites positively associated with trained immunity revealed enrichment of the tricarboxylic acid (TCA) cycle and glutamine metabolism, both of which were previously found to be important for trained immunity. Several new metabolic pathways that influence trained immunity were identified, among which taurine metabolism associated with BCG-induced trained immunity, a finding validated in functional experiments. In conclusion, circulating metabolites are important factors influencing BCG-induced trained immunity in humans. Modulation of metabolic pathways may be a novel strategy to improve vaccine and trained immunity responses. Public Library of Science 2022-09-12 /pmc/articles/PMC9499240/ /pubmed/36094960 http://dx.doi.org/10.1371/journal.pbio.3001765 Text en © 2022 Koeken et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Koeken, Valerie A. C. M.
Qi, Cancan
Mourits, Vera P.
de Bree, L. Charlotte J.
Moorlag, Simone J. C. F. M.
Sonawane, Vidhisha
Lemmers, Heidi
Dijkstra, Helga
Joosten, Leo A. B.
van Laarhoven, Arjan
Xu, Cheng-Jian
van Crevel, Reinout
Netea, Mihai G.
Li, Yang
Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination
title Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination
title_full Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination
title_fullStr Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination
title_full_unstemmed Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination
title_short Plasma metabolome predicts trained immunity responses after antituberculosis BCG vaccination
title_sort plasma metabolome predicts trained immunity responses after antituberculosis bcg vaccination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499240/
https://www.ncbi.nlm.nih.gov/pubmed/36094960
http://dx.doi.org/10.1371/journal.pbio.3001765
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