Improved Bladder Tumor RNA Isolation from Archived Tissues Using Methylene Blue for Normalization, Multiplex RNA Hybridization, Sequencing and Subtyping

Methylene blue (MB) is a dye used for histology with clinical importance and intercalates into nucleic acids. After MB staining of formalin fixed paraffin embedded (FFPE) muscle invasive bladder cancer (MIBC) and normal urothelium, specific regions could be microdissected. It is not known if MB infl...

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Detalles Bibliográficos
Autores principales: Köhler, Stefanie A., Brandl, Lisa, Strissel, Pamela L., Gloßner, Laura, Ekici, Arif B., Angeloni, Miriam, Ferrazzi, Fulvia, Bahlinger, Veronika, Hartmann, Arndt, Beckmann, Matthias W., Eckstein, Markus, Strick, Reiner
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499321/
https://www.ncbi.nlm.nih.gov/pubmed/36142180
http://dx.doi.org/10.3390/ijms231810267

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