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The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat

Cadmium (Cd) is a heavy metal nonessential for plants; this toxic metal accumulation in crops has significant adverse effects on human health. The crosstalk between copper (Cu) and Cd has been reported; however, the molecular mechanisms remain unknown. The present study investigated the function of...

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Autores principales: Liu, Xiaojuan, Wang, Hongcheng, He, Fang, Du, Xuye, Ren, Mingjian, Bao, Yinguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499326/
https://www.ncbi.nlm.nih.gov/pubmed/36142291
http://dx.doi.org/10.3390/ijms231810379
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author Liu, Xiaojuan
Wang, Hongcheng
He, Fang
Du, Xuye
Ren, Mingjian
Bao, Yinguang
author_facet Liu, Xiaojuan
Wang, Hongcheng
He, Fang
Du, Xuye
Ren, Mingjian
Bao, Yinguang
author_sort Liu, Xiaojuan
collection PubMed
description Cadmium (Cd) is a heavy metal nonessential for plants; this toxic metal accumulation in crops has significant adverse effects on human health. The crosstalk between copper (Cu) and Cd has been reported; however, the molecular mechanisms remain unknown. The present study investigated the function of wheat Cu transporter 3D (TaCOPT3D) in Cd tolerance. The TaCOPT3D transcripts significantly accumulated in wheat roots under Cd stress. Furthermore, TaCOPT3D-overexpressing lines were compared to wildtype (WT) plants to test the role of TaCOPT3D in Cd stress response. Under 20 mM Cd treatment, TaCOPT3D-overexpressing lines exhibited more biomass and lower root, shoot, and grain Cd accumulation than the WT plants. In addition, overexpression of TaCOPT3D decreased the reactive oxygen species (ROS) levels and increased the active antioxidant enzymes under Cd conditions. Moreover, the transcription factor (TF) TaWRKY22, which targeted the TaCOPT3D promoter, was identified in the regulatory pathway of TaCOPT3D under Cd stress. Taken together, these results show that TaCOPT3D plays an important role in regulating plant adaptation to cadmium stress through bound by TaWRKY22. These findings suggest that TaCOPT3D is a potential candidate for decreasing Cd accumulation in wheat through genetic engineering.
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spelling pubmed-94993262022-09-23 The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat Liu, Xiaojuan Wang, Hongcheng He, Fang Du, Xuye Ren, Mingjian Bao, Yinguang Int J Mol Sci Article Cadmium (Cd) is a heavy metal nonessential for plants; this toxic metal accumulation in crops has significant adverse effects on human health. The crosstalk between copper (Cu) and Cd has been reported; however, the molecular mechanisms remain unknown. The present study investigated the function of wheat Cu transporter 3D (TaCOPT3D) in Cd tolerance. The TaCOPT3D transcripts significantly accumulated in wheat roots under Cd stress. Furthermore, TaCOPT3D-overexpressing lines were compared to wildtype (WT) plants to test the role of TaCOPT3D in Cd stress response. Under 20 mM Cd treatment, TaCOPT3D-overexpressing lines exhibited more biomass and lower root, shoot, and grain Cd accumulation than the WT plants. In addition, overexpression of TaCOPT3D decreased the reactive oxygen species (ROS) levels and increased the active antioxidant enzymes under Cd conditions. Moreover, the transcription factor (TF) TaWRKY22, which targeted the TaCOPT3D promoter, was identified in the regulatory pathway of TaCOPT3D under Cd stress. Taken together, these results show that TaCOPT3D plays an important role in regulating plant adaptation to cadmium stress through bound by TaWRKY22. These findings suggest that TaCOPT3D is a potential candidate for decreasing Cd accumulation in wheat through genetic engineering. MDPI 2022-09-08 /pmc/articles/PMC9499326/ /pubmed/36142291 http://dx.doi.org/10.3390/ijms231810379 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Xiaojuan
Wang, Hongcheng
He, Fang
Du, Xuye
Ren, Mingjian
Bao, Yinguang
The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat
title The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat
title_full The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat
title_fullStr The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat
title_full_unstemmed The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat
title_short The TaWRKY22–TaCOPT3D Pathway Governs Cadmium Uptake in Wheat
title_sort tawrky22–tacopt3d pathway governs cadmium uptake in wheat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499326/
https://www.ncbi.nlm.nih.gov/pubmed/36142291
http://dx.doi.org/10.3390/ijms231810379
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