Cargando…

Mapping the Nicotinic Acetylcholine Receptor Nanocluster Topography at the Cell Membrane with STED and STORM Nanoscopies

The cell-surface topography and density of nicotinic acetylcholine receptors (nAChRs) play a key functional role in the synapse. Here we employ in parallel two labeling and two super-resolution microscopy strategies to characterize the distribution of this receptor at the plasma membrane of the mamm...

Descripción completa

Detalles Bibliográficos
Autores principales:	Saavedra, Lucas A., Buena-Maizón, Héctor, Barrantes, Francisco J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499342/ https://www.ncbi.nlm.nih.gov/pubmed/36142349 http://dx.doi.org/10.3390/ijms231810435

Ejemplares similares

STED nanoscopy: a glimpse into the future
por: Bianchini, Paolo, et al.
Publicado: (2015)

STED nanoscopy with fluorescent quantum dots
por: Hanne, Janina, et al.
Publicado: (2015)

Fluorescent Probes for STED Optical Nanoscopy
por: Jeong, Sejoo, et al.
Publicado: (2021)

Possible implications of dysregulated nicotinic acetylcholine receptor diffusion and nanocluster formation in myasthenia gravis
por: Barrantes, Francisco J.
Publicado: (2020)

Live-cell STED nanoscopy of mitochondrial cristae
por: Stephan, Till, et al.
Publicado: (2019)

Near-infrared STED nanoscopy with an engineered bacterial phytochrome
por: Kamper, Maria, et al.
Publicado: (2018)

STED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects
por: Vicidomini, Giuseppe, et al.
Publicado: (2013)

Tissue Multicolor STED Nanoscopy of Presynaptic Proteins in the Calyx of Held
por: Kempf, Christian, et al.
Publicado: (2013)

Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses
por: Sidenstein, Sven C., et al.
Publicado: (2016)

Coma aberrations in combined two- and three-dimensional STED nanoscopy
por: Antonello, Jacopo, et al.
Publicado: (2016)

Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain
por: Liu, Tianyan, et al.
Publicado: (2022)

Choosing dyes for cw-STED nanoscopy using self-assembled nanorulers
por: Beater, Susanne, et al.
Publicado: (2014)

Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy
por: Steshenko, Olena, et al.
Publicado: (2016)

Fluorescence Nanoscopy of Single DNA Molecules by Using Stimulated Emission Depletion (STED)**
por: Persson, F, et al.
Publicado: (2011)

Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe
por: Yang, Xusan, et al.
Publicado: (2020)

Environmental enrichment enhances patterning and remodeling of synaptic nanoarchitecture as revealed by STED nanoscopy
por: Wegner, Waja, et al.
Publicado: (2022)

Cell-surface translational dynamics of nicotinic acetylcholine receptors
por: Barrantes, Francisco J.
Publicado: (2014)

Dual channel STED nanoscopy of lytic granules on actin filaments in natural killer cells
por: Mace, Emily M., et al.
Publicado: (2012)

Achieving λ/10 Resolution CW STED Nanoscopy with a Ti:Sapphire Oscillator
por: Liu, Yujia, et al.
Publicado: (2012)

Photobleaching in STED nanoscopy and its dependence on the photon flux applied for reversible silencing of the fluorophore
por: Oracz, Joanna, et al.
Publicado: (2017)

Guided STED nanoscopy enables super-resolution imaging of blood stage malaria parasites
por: Schloetel, Jan-Gero, et al.
Publicado: (2019)

AFM-STED correlative nanoscopy reveals a dark side in fluorescence microscopy imaging
por: Cosentino, Michela, et al.
Publicado: (2019)

Multicolour nanoscopy of fixed and living cells with a single STED beam and hyperspectral detection
por: Winter, Franziska R., et al.
Publicado: (2017)

Membrane Hsp70-supported cell-to-cell connections via tunneling nanotubes revealed by live-cell STED nanoscopy
por: Reindl, Judith, et al.
Publicado: (2019)

Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
por: Mosqueira, Alejo, et al.
Publicado: (2018)

Mechanics of Channel Gating of the Nicotinic Acetylcholine Receptor
por: Liu, Xinli, et al.
Publicado: (2008)

Activity-Dependent Remodeling of Synaptic Protein Organization Revealed by High Throughput Analysis of STED Nanoscopy Images
por: Wiesner, Theresa, et al.
Publicado: (2020)

Transient Cholesterol Effects on Nicotinic Acetylcholine Receptor Cell-Surface Mobility
por: Almarza, Gonzalo, et al.
Publicado: (2014)

Nicotinic Acetylcholine Receptor Dysfunction in Addiction and in Some Neurodegenerative and Neuropsychiatric Diseases
por: Vallés, Ana Sofía, et al.
Publicado: (2023)

Novel reversibly switchable fluorescent proteins for RESOLFT and STED nanoscopy engineered from the bacterial photoreceptor YtvA
por: Gregor, Carola, et al.
Publicado: (2018)

Stable but not rigid: Chronic in vivo STED nanoscopy reveals extensive remodeling of spines, indicating multiple drivers of plasticity
por: Steffens, Heinz, et al.
Publicado: (2021)

Dysregulation of Neuronal Nicotinic Acetylcholine Receptor–Cholesterol Crosstalk in Autism Spectrum Disorder
por: Vallés, Ana Sofía, et al.
Publicado: (2021)

Disclosure of cholesterol recognition motifs in transmembrane domains of the human nicotinic acetylcholine receptor
por: Baier, Carlos J., et al.
Publicado: (2011)

Homomeric and Heteromeric α7 Nicotinic Acetylcholine Receptors in Health and Some Central Nervous System Diseases
por: Borroni, Virginia, et al.
Publicado: (2021)

WindSTORM: Robust online image processing for high-throughput nanoscopy
por: Ma, Hongqiang, et al.
Publicado: (2019)

Structural model of nicotinic acetylcholine receptor isotypes bound to acetylcholine and nicotine
por: Schapira, Matthieu, et al.
Publicado: (2002)

Nicotinic acetylcholine receptor is internalized via a Rac-dependent, dynamin-independent endocytic pathway
por: Kumari, Sudha, et al.
Publicado: (2008)

STED nanoscopy of the centrosome linker reveals a CEP68-organized, periodic rootletin network anchored to a C-Nap1 ring at centrioles
por: Vlijm, Rifka, et al.
Publicado: (2018)

Lovastatin Differentially Regulates α7 and α4 Neuronal Nicotinic Acetylcholine Receptor Levels in Rat Hippocampal Neurons
por: Borroni, Virginia, et al.
Publicado: (2020)

Interactions between the Nicotinic and Endocannabinoid Receptors at the Plasma Membrane
por: Vallés, Ana Sofía, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_c8esl8t9mhks627pjdr4r12g2d