Cargando…

Erythrocyte-Derived Nanoparticles with Folate Functionalization for Near Infrared Pulsed Laser-Mediated Photo-Chemotherapy of Tumors

Despite its common side effects and varying degrees of therapeutic success, chemotherapy remains the gold standard method for treatment of cancer. Towards developing a new therapeutic approach, we have engineered nanoparticles derived from erythrocytes that contain indocyanine green as a photo-activ...

Descripción completa

Detalles Bibliográficos
Autores principales: Mac, Jenny T., Vankayala, Raviraj, Lee, Chi-Hua, Anvari, Bahman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499474/
https://www.ncbi.nlm.nih.gov/pubmed/36142205
http://dx.doi.org/10.3390/ijms231810295
_version_ 1784795000005984256
author Mac, Jenny T.
Vankayala, Raviraj
Lee, Chi-Hua
Anvari, Bahman
author_facet Mac, Jenny T.
Vankayala, Raviraj
Lee, Chi-Hua
Anvari, Bahman
author_sort Mac, Jenny T.
collection PubMed
description Despite its common side effects and varying degrees of therapeutic success, chemotherapy remains the gold standard method for treatment of cancer. Towards developing a new therapeutic approach, we have engineered nanoparticles derived from erythrocytes that contain indocyanine green as a photo-activated agent that enables near infrared photothermal heating, and doxorubicin hydrochloride (DOX) as a chemotherapeutic drug. We hypothesize that milliseconds pulsed laser irradiation results in rapid heating and photo-triggered release of DOX, providing a dual photo-chemo therapeutic mechanism for tumor destruction. Additionally, the surface of the nanoparticles is functionalized with folate to target the folate receptor-α on tumor cells to further enhance the therapeutic efficacy. Using non-contract infrared radiometry and absorption spectroscopy, we have characterized the photothermal response and photostability of the nanoparticles to pulsed laser irradiation. Our in vitro studies show that these nanoparticles can mediate photo-chemo killing of SKOV3 ovarian cancer cells when activated by pulsed laser irradiation. We further demonstrate that this dual photo-chemo therapeutic approach is effective in reducing the volume of tumor implants in mice and elicits an apoptotic response. This treatment modality presents a promising approach in destruction of small tumor nodules.
format Online
Article
Text
id pubmed-9499474
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-94994742022-09-23 Erythrocyte-Derived Nanoparticles with Folate Functionalization for Near Infrared Pulsed Laser-Mediated Photo-Chemotherapy of Tumors Mac, Jenny T. Vankayala, Raviraj Lee, Chi-Hua Anvari, Bahman Int J Mol Sci Article Despite its common side effects and varying degrees of therapeutic success, chemotherapy remains the gold standard method for treatment of cancer. Towards developing a new therapeutic approach, we have engineered nanoparticles derived from erythrocytes that contain indocyanine green as a photo-activated agent that enables near infrared photothermal heating, and doxorubicin hydrochloride (DOX) as a chemotherapeutic drug. We hypothesize that milliseconds pulsed laser irradiation results in rapid heating and photo-triggered release of DOX, providing a dual photo-chemo therapeutic mechanism for tumor destruction. Additionally, the surface of the nanoparticles is functionalized with folate to target the folate receptor-α on tumor cells to further enhance the therapeutic efficacy. Using non-contract infrared radiometry and absorption spectroscopy, we have characterized the photothermal response and photostability of the nanoparticles to pulsed laser irradiation. Our in vitro studies show that these nanoparticles can mediate photo-chemo killing of SKOV3 ovarian cancer cells when activated by pulsed laser irradiation. We further demonstrate that this dual photo-chemo therapeutic approach is effective in reducing the volume of tumor implants in mice and elicits an apoptotic response. This treatment modality presents a promising approach in destruction of small tumor nodules. MDPI 2022-09-07 /pmc/articles/PMC9499474/ /pubmed/36142205 http://dx.doi.org/10.3390/ijms231810295 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mac, Jenny T.
Vankayala, Raviraj
Lee, Chi-Hua
Anvari, Bahman
Erythrocyte-Derived Nanoparticles with Folate Functionalization for Near Infrared Pulsed Laser-Mediated Photo-Chemotherapy of Tumors
title Erythrocyte-Derived Nanoparticles with Folate Functionalization for Near Infrared Pulsed Laser-Mediated Photo-Chemotherapy of Tumors
title_full Erythrocyte-Derived Nanoparticles with Folate Functionalization for Near Infrared Pulsed Laser-Mediated Photo-Chemotherapy of Tumors
title_fullStr Erythrocyte-Derived Nanoparticles with Folate Functionalization for Near Infrared Pulsed Laser-Mediated Photo-Chemotherapy of Tumors
title_full_unstemmed Erythrocyte-Derived Nanoparticles with Folate Functionalization for Near Infrared Pulsed Laser-Mediated Photo-Chemotherapy of Tumors
title_short Erythrocyte-Derived Nanoparticles with Folate Functionalization for Near Infrared Pulsed Laser-Mediated Photo-Chemotherapy of Tumors
title_sort erythrocyte-derived nanoparticles with folate functionalization for near infrared pulsed laser-mediated photo-chemotherapy of tumors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499474/
https://www.ncbi.nlm.nih.gov/pubmed/36142205
http://dx.doi.org/10.3390/ijms231810295
work_keys_str_mv AT macjennyt erythrocytederivednanoparticleswithfolatefunctionalizationfornearinfraredpulsedlasermediatedphotochemotherapyoftumors
AT vankayalaraviraj erythrocytederivednanoparticleswithfolatefunctionalizationfornearinfraredpulsedlasermediatedphotochemotherapyoftumors
AT leechihua erythrocytederivednanoparticleswithfolatefunctionalizationfornearinfraredpulsedlasermediatedphotochemotherapyoftumors
AT anvaribahman erythrocytederivednanoparticleswithfolatefunctionalizationfornearinfraredpulsedlasermediatedphotochemotherapyoftumors