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The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages

The aim of this study was to evaluate the levels of ten energy metabolism factors: C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 (total), resistin, and visfatin, and to determine the expression of GLP1R receptors, CD10, CD26 proteases, and pro-inflammatory marker CD86 by macrophag...

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Autores principales: Krasnyi, Aleksey M., Sadekova, Alsu A., Smolnova, Tatyana Y., Chursin, Vyacheslav V., Buralkina, Natalya A., Chuprynin, Vladimir D., Yarotskaya, Ekaterina, Pavlovich, Stanislav V., Sukhikh, Gennadiy T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499521/
https://www.ncbi.nlm.nih.gov/pubmed/36142272
http://dx.doi.org/10.3390/ijms231810361
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author Krasnyi, Aleksey M.
Sadekova, Alsu A.
Smolnova, Tatyana Y.
Chursin, Vyacheslav V.
Buralkina, Natalya A.
Chuprynin, Vladimir D.
Yarotskaya, Ekaterina
Pavlovich, Stanislav V.
Sukhikh, Gennadiy T.
author_facet Krasnyi, Aleksey M.
Sadekova, Alsu A.
Smolnova, Tatyana Y.
Chursin, Vyacheslav V.
Buralkina, Natalya A.
Chuprynin, Vladimir D.
Yarotskaya, Ekaterina
Pavlovich, Stanislav V.
Sukhikh, Gennadiy T.
author_sort Krasnyi, Aleksey M.
collection PubMed
description The aim of this study was to evaluate the levels of ten energy metabolism factors: C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 (total), resistin, and visfatin, and to determine the expression of GLP1R receptors, CD10, CD26 proteases, and pro-inflammatory marker CD86 by macrophages in the peritoneal fluid (PF) in patients with endometriosis. The study included 54 women with endometriosis and a control group of 30 women with uterine myoma without signs of endometriosis. The levels of factors in PF were assessed by a multiplex method. Expression of GLP1R receptors, CD10, CD26 proteases, and CD86 by macrophages was evaluated using flow cytometry. It was found that in women with endometriosis, the concentrations of ghrelin, GLP-1, glucagon, and visfatin in PF were reduced (p = 0.007, p = 0.009, p = 0.002, p = 0.008, respectively). At the same time, there was a noted increase in the CD10 protease expression by peritoneal macrophages (p = 0.044). Correlation analysis showed a positive correlation of ghrelin and GLP-1 levels with CD86 macrophage expression (p = 0.044, p = 0.022, respectively) in the study group; a positive correlation was also found between the levels of GLP-1, glucagon, and visfatin with CD26 macrophage expression (p = 0.041, p = 0.048, p = 0.015, respectively) in PF. No correlations were found in the control group. These results indicate that a decrease in the levels of ghrelin, GLP-1, glucagon, and visfatin in PF may contribute to endometriosis development through their impact on the expression of pro-inflammatory markers of PF macrophages.
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spelling pubmed-94995212022-09-23 The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages Krasnyi, Aleksey M. Sadekova, Alsu A. Smolnova, Tatyana Y. Chursin, Vyacheslav V. Buralkina, Natalya A. Chuprynin, Vladimir D. Yarotskaya, Ekaterina Pavlovich, Stanislav V. Sukhikh, Gennadiy T. Int J Mol Sci Article The aim of this study was to evaluate the levels of ten energy metabolism factors: C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 (total), resistin, and visfatin, and to determine the expression of GLP1R receptors, CD10, CD26 proteases, and pro-inflammatory marker CD86 by macrophages in the peritoneal fluid (PF) in patients with endometriosis. The study included 54 women with endometriosis and a control group of 30 women with uterine myoma without signs of endometriosis. The levels of factors in PF were assessed by a multiplex method. Expression of GLP1R receptors, CD10, CD26 proteases, and CD86 by macrophages was evaluated using flow cytometry. It was found that in women with endometriosis, the concentrations of ghrelin, GLP-1, glucagon, and visfatin in PF were reduced (p = 0.007, p = 0.009, p = 0.002, p = 0.008, respectively). At the same time, there was a noted increase in the CD10 protease expression by peritoneal macrophages (p = 0.044). Correlation analysis showed a positive correlation of ghrelin and GLP-1 levels with CD86 macrophage expression (p = 0.044, p = 0.022, respectively) in the study group; a positive correlation was also found between the levels of GLP-1, glucagon, and visfatin with CD26 macrophage expression (p = 0.041, p = 0.048, p = 0.015, respectively) in PF. No correlations were found in the control group. These results indicate that a decrease in the levels of ghrelin, GLP-1, glucagon, and visfatin in PF may contribute to endometriosis development through their impact on the expression of pro-inflammatory markers of PF macrophages. MDPI 2022-09-08 /pmc/articles/PMC9499521/ /pubmed/36142272 http://dx.doi.org/10.3390/ijms231810361 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krasnyi, Aleksey M.
Sadekova, Alsu A.
Smolnova, Tatyana Y.
Chursin, Vyacheslav V.
Buralkina, Natalya A.
Chuprynin, Vladimir D.
Yarotskaya, Ekaterina
Pavlovich, Stanislav V.
Sukhikh, Gennadiy T.
The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages
title The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages
title_full The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages
title_fullStr The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages
title_full_unstemmed The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages
title_short The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages
title_sort levels of ghrelin, glucagon, visfatin and glp-1 are decreased in the peritoneal fluid of women with endometriosis along with the increased expression of the cd10 protease by the macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499521/
https://www.ncbi.nlm.nih.gov/pubmed/36142272
http://dx.doi.org/10.3390/ijms231810361
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