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Virus-Induced Gene Editing and Its Applications in Plants

CRISPR/Cas-based genome editing technologies, which allow the precise manipulation of plant genomes, have revolutionized plant science and enabled the creation of germplasms with beneficial traits. In order to apply these technologies, CRISPR/Cas reagents must be delivered into plant cells; however,...

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Detalles Bibliográficos
Autores principales: Zhang, Chao, Liu, Shanhe, Li, Xuan, Zhang, Ruixuan, Li, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499690/
https://www.ncbi.nlm.nih.gov/pubmed/36142116
http://dx.doi.org/10.3390/ijms231810202
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author Zhang, Chao
Liu, Shanhe
Li, Xuan
Zhang, Ruixuan
Li, Jun
author_facet Zhang, Chao
Liu, Shanhe
Li, Xuan
Zhang, Ruixuan
Li, Jun
author_sort Zhang, Chao
collection PubMed
description CRISPR/Cas-based genome editing technologies, which allow the precise manipulation of plant genomes, have revolutionized plant science and enabled the creation of germplasms with beneficial traits. In order to apply these technologies, CRISPR/Cas reagents must be delivered into plant cells; however, this is limited by tissue culture challenges. Recently, viral vectors have been used to deliver CRISPR/Cas reagents into plant cells. Virus-induced genome editing (VIGE) has emerged as a powerful method with several advantages, including high editing efficiency and a simplified process for generating gene-edited DNA-free plants. Here, we briefly describe CRISPR/Cas-based genome editing. We then focus on VIGE systems and the types of viruses used currently for CRISPR/Cas9 cassette delivery and genome editing. We also highlight recent applications of and advances in VIGE in plants. Finally, we discuss the challenges and potential for VIGE in plants.
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spelling pubmed-94996902022-09-23 Virus-Induced Gene Editing and Its Applications in Plants Zhang, Chao Liu, Shanhe Li, Xuan Zhang, Ruixuan Li, Jun Int J Mol Sci Review CRISPR/Cas-based genome editing technologies, which allow the precise manipulation of plant genomes, have revolutionized plant science and enabled the creation of germplasms with beneficial traits. In order to apply these technologies, CRISPR/Cas reagents must be delivered into plant cells; however, this is limited by tissue culture challenges. Recently, viral vectors have been used to deliver CRISPR/Cas reagents into plant cells. Virus-induced genome editing (VIGE) has emerged as a powerful method with several advantages, including high editing efficiency and a simplified process for generating gene-edited DNA-free plants. Here, we briefly describe CRISPR/Cas-based genome editing. We then focus on VIGE systems and the types of viruses used currently for CRISPR/Cas9 cassette delivery and genome editing. We also highlight recent applications of and advances in VIGE in plants. Finally, we discuss the challenges and potential for VIGE in plants. MDPI 2022-09-06 /pmc/articles/PMC9499690/ /pubmed/36142116 http://dx.doi.org/10.3390/ijms231810202 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Zhang, Chao
Liu, Shanhe
Li, Xuan
Zhang, Ruixuan
Li, Jun
Virus-Induced Gene Editing and Its Applications in Plants
title Virus-Induced Gene Editing and Its Applications in Plants
title_full Virus-Induced Gene Editing and Its Applications in Plants
title_fullStr Virus-Induced Gene Editing and Its Applications in Plants
title_full_unstemmed Virus-Induced Gene Editing and Its Applications in Plants
title_short Virus-Induced Gene Editing and Its Applications in Plants
title_sort virus-induced gene editing and its applications in plants
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499690/
https://www.ncbi.nlm.nih.gov/pubmed/36142116
http://dx.doi.org/10.3390/ijms231810202
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