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An appressorium membrane protein, Pams1, controls infection structure maturation and virulence via maintaining endosomal stability in the rice blast fungus

The rice blast fungus Magnaporthe oryzae spores differentiate and mature into functional appressoria by sensing the host surface signals. Environmental stimuli are transduced into cells through internalization during appressorium formation, such as in the cAMP-PKA pathway. Here, we describe a novel...

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Autores principales: Wang, Jing, Wang, Qing, Huang, Pengyun, Qu, Yingmin, Huang, Zhicheng, Wang, Huan, Liu, Xiao-Hong, Lin, Fu-Cheng, Lu, Jianping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500233/
https://www.ncbi.nlm.nih.gov/pubmed/36160954
http://dx.doi.org/10.3389/fpls.2022.955254
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author Wang, Jing
Wang, Qing
Huang, Pengyun
Qu, Yingmin
Huang, Zhicheng
Wang, Huan
Liu, Xiao-Hong
Lin, Fu-Cheng
Lu, Jianping
author_facet Wang, Jing
Wang, Qing
Huang, Pengyun
Qu, Yingmin
Huang, Zhicheng
Wang, Huan
Liu, Xiao-Hong
Lin, Fu-Cheng
Lu, Jianping
author_sort Wang, Jing
collection PubMed
description The rice blast fungus Magnaporthe oryzae spores differentiate and mature into functional appressoria by sensing the host surface signals. Environmental stimuli are transduced into cells through internalization during appressorium formation, such as in the cAMP-PKA pathway. Here, we describe a novel contribution to how appressoria mature on the surface of a leaf, and its connection to endosomes and the cAMP-PKA pathway. An appressorium membrane-specific protein, Pams1, is required for maintaining endosomal structure, appressorium maturation, and virulence in M. oryzae. During appressorium development, Pams1 was translocated from the cell membrane to the endosomal membrane. Deletion of PAMS1 led to the formation of two types of abnormal appressoria after 8 h post inoculation (hpi): melanized type I had a reduced virulence, while pale type II was dead. Before 8 hpi, Δpams1 formed appressoria that were similar to those of the wild type. After 8 hpi, the appressoria of Δpams1 was differentiated into two types: (1) the cell walls of type I appressoria were melanized, endosomes were larger, and had a different distribution from the wild type and (2) Type II appressoria gradually stopped melanization and began to die. The organelles, including the nucleus, endosomes, mitochondria, and endoplasmic reticula, were degraded, leaving only autophagic body-like vesicles in type II appressoria. The addition of exogenous cAMP to Δpams1 led to the formation of a greater proportion of type I appressoria and a smaller proportion of type II appressoria. Thus, defects in endosomal structure and the cAMP-PKA pathway are among the causes of the defective appressorium maturation and virulence of Δpams1.
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spelling pubmed-95002332022-09-24 An appressorium membrane protein, Pams1, controls infection structure maturation and virulence via maintaining endosomal stability in the rice blast fungus Wang, Jing Wang, Qing Huang, Pengyun Qu, Yingmin Huang, Zhicheng Wang, Huan Liu, Xiao-Hong Lin, Fu-Cheng Lu, Jianping Front Plant Sci Plant Science The rice blast fungus Magnaporthe oryzae spores differentiate and mature into functional appressoria by sensing the host surface signals. Environmental stimuli are transduced into cells through internalization during appressorium formation, such as in the cAMP-PKA pathway. Here, we describe a novel contribution to how appressoria mature on the surface of a leaf, and its connection to endosomes and the cAMP-PKA pathway. An appressorium membrane-specific protein, Pams1, is required for maintaining endosomal structure, appressorium maturation, and virulence in M. oryzae. During appressorium development, Pams1 was translocated from the cell membrane to the endosomal membrane. Deletion of PAMS1 led to the formation of two types of abnormal appressoria after 8 h post inoculation (hpi): melanized type I had a reduced virulence, while pale type II was dead. Before 8 hpi, Δpams1 formed appressoria that were similar to those of the wild type. After 8 hpi, the appressoria of Δpams1 was differentiated into two types: (1) the cell walls of type I appressoria were melanized, endosomes were larger, and had a different distribution from the wild type and (2) Type II appressoria gradually stopped melanization and began to die. The organelles, including the nucleus, endosomes, mitochondria, and endoplasmic reticula, were degraded, leaving only autophagic body-like vesicles in type II appressoria. The addition of exogenous cAMP to Δpams1 led to the formation of a greater proportion of type I appressoria and a smaller proportion of type II appressoria. Thus, defects in endosomal structure and the cAMP-PKA pathway are among the causes of the defective appressorium maturation and virulence of Δpams1. Frontiers Media S.A. 2022-09-09 /pmc/articles/PMC9500233/ /pubmed/36160954 http://dx.doi.org/10.3389/fpls.2022.955254 Text en Copyright © 2022 Wang, Wang, Huang, Qu, Huang, Wang, Liu, Lin and Lu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Jing
Wang, Qing
Huang, Pengyun
Qu, Yingmin
Huang, Zhicheng
Wang, Huan
Liu, Xiao-Hong
Lin, Fu-Cheng
Lu, Jianping
An appressorium membrane protein, Pams1, controls infection structure maturation and virulence via maintaining endosomal stability in the rice blast fungus
title An appressorium membrane protein, Pams1, controls infection structure maturation and virulence via maintaining endosomal stability in the rice blast fungus
title_full An appressorium membrane protein, Pams1, controls infection structure maturation and virulence via maintaining endosomal stability in the rice blast fungus
title_fullStr An appressorium membrane protein, Pams1, controls infection structure maturation and virulence via maintaining endosomal stability in the rice blast fungus
title_full_unstemmed An appressorium membrane protein, Pams1, controls infection structure maturation and virulence via maintaining endosomal stability in the rice blast fungus
title_short An appressorium membrane protein, Pams1, controls infection structure maturation and virulence via maintaining endosomal stability in the rice blast fungus
title_sort appressorium membrane protein, pams1, controls infection structure maturation and virulence via maintaining endosomal stability in the rice blast fungus
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500233/
https://www.ncbi.nlm.nih.gov/pubmed/36160954
http://dx.doi.org/10.3389/fpls.2022.955254
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