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Radiation Response of Human Leukemia/Lymphoma Cells was Improved by 7-Geranyloxycoumarin

OBJECTIVES: Adult T-cell leukemia/lymphoma (ATLL) is a blood neoplasm with specific geographic distribution. Although radiotherapy is a palliative treatment that provides long-term local control, single use of radiation leads to complications for patients. To introduce a novel multimodal approach ag...

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Autores principales: Bagheri, Ramin, Rassouli, Fatemeh B., Gholamhosseinian, Hamid, Ebrahimi, Keyhan, Mahdavi, Shakiba, Goudarzi, Sajad, Iranshahi, Mehrdad, Rafatpanah, Houshang, Keramati, Mohammad Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500271/
https://www.ncbi.nlm.nih.gov/pubmed/36158737
http://dx.doi.org/10.1177/15593258221124479
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author Bagheri, Ramin
Rassouli, Fatemeh B.
Gholamhosseinian, Hamid
Ebrahimi, Keyhan
Mahdavi, Shakiba
Goudarzi, Sajad
Iranshahi, Mehrdad
Rafatpanah, Houshang
Keramati, Mohammad Reza
author_facet Bagheri, Ramin
Rassouli, Fatemeh B.
Gholamhosseinian, Hamid
Ebrahimi, Keyhan
Mahdavi, Shakiba
Goudarzi, Sajad
Iranshahi, Mehrdad
Rafatpanah, Houshang
Keramati, Mohammad Reza
author_sort Bagheri, Ramin
collection PubMed
description OBJECTIVES: Adult T-cell leukemia/lymphoma (ATLL) is a blood neoplasm with specific geographic distribution. Although radiotherapy is a palliative treatment that provides long-term local control, single use of radiation leads to complications for patients. To introduce a novel multimodal approach against ATLL, we investigated combinatorial effects of 7-geranyloxycoumarin and radiation in vitro. METHODS: Viability of MT-2 cells was determined by resazurin assay upon administration of 7-geranyloxycoumarin alone and followed by radiation. Then, apoptosis was detected by annexin V and propidium iodide, and the expression of candidate genes was analyzed by qPCR. RESULTS: Findings revealed significant (P<.0001) improvement in radiation effects upon 7-geranyloxycoumarin pretreatment, most notably when cells were pretreated with 5 µg/ml 7-geranyloxycoumarin for 96 h, exposed to 6 Gy radiation and recovered for 48 h. These results were confirmed by flow cytometry, as the percentage of early and late apoptotic cells was increased after combinatorial treatment. In addition, significant (P< .0001) changes in CD44, c-MYC, cFLIPL, BMI-1, NF-κB (Rel A), and P53 expression was induced by 7-geranyloxycoumarin and radiation. CONCLUSIONS: Current research indicated, for the first time, that combinatorial use of 7-geranyloxycoumarin and ionizing radiation could be considered as an effective therapeutic modality for ATLL.
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spelling pubmed-95002712022-09-24 Radiation Response of Human Leukemia/Lymphoma Cells was Improved by 7-Geranyloxycoumarin Bagheri, Ramin Rassouli, Fatemeh B. Gholamhosseinian, Hamid Ebrahimi, Keyhan Mahdavi, Shakiba Goudarzi, Sajad Iranshahi, Mehrdad Rafatpanah, Houshang Keramati, Mohammad Reza Dose Response Original Article OBJECTIVES: Adult T-cell leukemia/lymphoma (ATLL) is a blood neoplasm with specific geographic distribution. Although radiotherapy is a palliative treatment that provides long-term local control, single use of radiation leads to complications for patients. To introduce a novel multimodal approach against ATLL, we investigated combinatorial effects of 7-geranyloxycoumarin and radiation in vitro. METHODS: Viability of MT-2 cells was determined by resazurin assay upon administration of 7-geranyloxycoumarin alone and followed by radiation. Then, apoptosis was detected by annexin V and propidium iodide, and the expression of candidate genes was analyzed by qPCR. RESULTS: Findings revealed significant (P<.0001) improvement in radiation effects upon 7-geranyloxycoumarin pretreatment, most notably when cells were pretreated with 5 µg/ml 7-geranyloxycoumarin for 96 h, exposed to 6 Gy radiation and recovered for 48 h. These results were confirmed by flow cytometry, as the percentage of early and late apoptotic cells was increased after combinatorial treatment. In addition, significant (P< .0001) changes in CD44, c-MYC, cFLIPL, BMI-1, NF-κB (Rel A), and P53 expression was induced by 7-geranyloxycoumarin and radiation. CONCLUSIONS: Current research indicated, for the first time, that combinatorial use of 7-geranyloxycoumarin and ionizing radiation could be considered as an effective therapeutic modality for ATLL. SAGE Publications 2022-09-21 /pmc/articles/PMC9500271/ /pubmed/36158737 http://dx.doi.org/10.1177/15593258221124479 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Bagheri, Ramin
Rassouli, Fatemeh B.
Gholamhosseinian, Hamid
Ebrahimi, Keyhan
Mahdavi, Shakiba
Goudarzi, Sajad
Iranshahi, Mehrdad
Rafatpanah, Houshang
Keramati, Mohammad Reza
Radiation Response of Human Leukemia/Lymphoma Cells was Improved by 7-Geranyloxycoumarin
title Radiation Response of Human Leukemia/Lymphoma Cells was Improved by 7-Geranyloxycoumarin
title_full Radiation Response of Human Leukemia/Lymphoma Cells was Improved by 7-Geranyloxycoumarin
title_fullStr Radiation Response of Human Leukemia/Lymphoma Cells was Improved by 7-Geranyloxycoumarin
title_full_unstemmed Radiation Response of Human Leukemia/Lymphoma Cells was Improved by 7-Geranyloxycoumarin
title_short Radiation Response of Human Leukemia/Lymphoma Cells was Improved by 7-Geranyloxycoumarin
title_sort radiation response of human leukemia/lymphoma cells was improved by 7-geranyloxycoumarin
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500271/
https://www.ncbi.nlm.nih.gov/pubmed/36158737
http://dx.doi.org/10.1177/15593258221124479
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