Evaluation of Cyto - and Genotoxic Influence of Lanthanum Dioxide Nanoparticles on Human Liver Cells

Inorganic nanoparticles are representing an emerging paradigm in molecular imaging probe design. We have determined lanthanum oxide nanoparticles (La(2)O(3) NPs)-induced toxicity on human livers cells for 48 hrs. Before exposure to La(2)O(3) NPs, the size and shape of NPs were confirmed by transmission electron microscope. It was found at 32 ±1.6 nm with a sheet-like morphological structure. The viability of CHANG and HuH-7 cells was reduced as the concentration of La(2)O(3) NPs increased. HuH-7 cells were more sensitive than CHANG cells to La(2)O(3) NPs. We observed production of intracellular ROS in HuH-7 cells was more than CHANG cells and the LPO level was more in CHANG cells than in HuH-7 cells at 50 μg/ml of La(2)O(3) NPs. Glutathione was decreased and catalase was increased at 50 μg/ml of La(2)O(3) NPs. More apoptotic and necrotic cells were observed at 300 μg/ml in HuH-7 cells FACS. DNA damage was observed by the SGCE test and more DNA damage was found in CHANG cells than HuH-7 cells at 300 μg/ml La(2)O(3) NPs over 48 hrs. Thus, study warrants the application of La(2)O(3) NPs in daily life and provides vital information about the toxicity of La(2)O(3) NPs.