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The neural stem cell properties of Pkd2l1(+) cerebrospinal fluid-contacting neurons in vivo

The neural stem cells (NSCs) in the ventricular-subventricular zone of the adult mammalian spinal cord may be of great benefit for repairing spinal cord injuries. However, the sources of NSCs remain unclear. Previously, we have confirmed that cerebrospinal fluid-contacting neurons (CSF-cNs) have NSC...

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Autores principales: Cao, Liang, Huang, Ming-Zhi, Zhang, Qiang, Luo, Zhang-Rong, Zhang, Yi, An, Ping-Jiang, Yang, Lei-Luo, Tan, Wei, Wang, Chun-Qing, Dou, Xiao-Wei, Li, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500444/
https://www.ncbi.nlm.nih.gov/pubmed/36159391
http://dx.doi.org/10.3389/fncel.2022.992520
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author Cao, Liang
Huang, Ming-Zhi
Zhang, Qiang
Luo, Zhang-Rong
Zhang, Yi
An, Ping-Jiang
Yang, Lei-Luo
Tan, Wei
Wang, Chun-Qing
Dou, Xiao-Wei
Li, Qing
author_facet Cao, Liang
Huang, Ming-Zhi
Zhang, Qiang
Luo, Zhang-Rong
Zhang, Yi
An, Ping-Jiang
Yang, Lei-Luo
Tan, Wei
Wang, Chun-Qing
Dou, Xiao-Wei
Li, Qing
author_sort Cao, Liang
collection PubMed
description The neural stem cells (NSCs) in the ventricular-subventricular zone of the adult mammalian spinal cord may be of great benefit for repairing spinal cord injuries. However, the sources of NSCs remain unclear. Previously, we have confirmed that cerebrospinal fluid-contacting neurons (CSF-cNs) have NSC potential in vitro. In this study, we verified the NSC properties of CSF-cNs in vivo. In mouse spinal cords, Pkd2l1(+) CSF-cNs localized around the central canal express NSC markers. In vitro, Pkd2l1(+) CSF-cNs form a neurosphere and express NSC markers. Activation and proliferation of CSF-cNs can be induced by injection of the neurotrophic factors basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) into the lateral ventricle. Spinal cord injury (SCI) also induces NSC activation and proliferation of CSF-cNs. Collectively, our results demonstrate that Pkd2l1(+) CSF-cNs have NSC properties in vivo and may be involved in SCI recovery.
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spelling pubmed-95004442022-09-24 The neural stem cell properties of Pkd2l1(+) cerebrospinal fluid-contacting neurons in vivo Cao, Liang Huang, Ming-Zhi Zhang, Qiang Luo, Zhang-Rong Zhang, Yi An, Ping-Jiang Yang, Lei-Luo Tan, Wei Wang, Chun-Qing Dou, Xiao-Wei Li, Qing Front Cell Neurosci Neuroscience The neural stem cells (NSCs) in the ventricular-subventricular zone of the adult mammalian spinal cord may be of great benefit for repairing spinal cord injuries. However, the sources of NSCs remain unclear. Previously, we have confirmed that cerebrospinal fluid-contacting neurons (CSF-cNs) have NSC potential in vitro. In this study, we verified the NSC properties of CSF-cNs in vivo. In mouse spinal cords, Pkd2l1(+) CSF-cNs localized around the central canal express NSC markers. In vitro, Pkd2l1(+) CSF-cNs form a neurosphere and express NSC markers. Activation and proliferation of CSF-cNs can be induced by injection of the neurotrophic factors basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) into the lateral ventricle. Spinal cord injury (SCI) also induces NSC activation and proliferation of CSF-cNs. Collectively, our results demonstrate that Pkd2l1(+) CSF-cNs have NSC properties in vivo and may be involved in SCI recovery. Frontiers Media S.A. 2022-09-09 /pmc/articles/PMC9500444/ /pubmed/36159391 http://dx.doi.org/10.3389/fncel.2022.992520 Text en Copyright © 2022 Cao, Huang, Zhang, Luo, Zhang, An, Yang, Tan, Wang, Dou and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Cao, Liang
Huang, Ming-Zhi
Zhang, Qiang
Luo, Zhang-Rong
Zhang, Yi
An, Ping-Jiang
Yang, Lei-Luo
Tan, Wei
Wang, Chun-Qing
Dou, Xiao-Wei
Li, Qing
The neural stem cell properties of Pkd2l1(+) cerebrospinal fluid-contacting neurons in vivo
title The neural stem cell properties of Pkd2l1(+) cerebrospinal fluid-contacting neurons in vivo
title_full The neural stem cell properties of Pkd2l1(+) cerebrospinal fluid-contacting neurons in vivo
title_fullStr The neural stem cell properties of Pkd2l1(+) cerebrospinal fluid-contacting neurons in vivo
title_full_unstemmed The neural stem cell properties of Pkd2l1(+) cerebrospinal fluid-contacting neurons in vivo
title_short The neural stem cell properties of Pkd2l1(+) cerebrospinal fluid-contacting neurons in vivo
title_sort neural stem cell properties of pkd2l1(+) cerebrospinal fluid-contacting neurons in vivo
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500444/
https://www.ncbi.nlm.nih.gov/pubmed/36159391
http://dx.doi.org/10.3389/fncel.2022.992520
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