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Anti-Trichomonas gallinae activity of essential oils and main compounds from Lamiaceae and Asteraceae plants

Trichomonas gallinae is a flagellated protozoan that parasitizes the upper digestive tract of various bird species and causes avian trichomonosis. The emergence of resistant strains to the standard treatment, based on nitroimidazoles, increases the need to find alternative therapies. In this study,...

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Autores principales: Bailén, María, Díaz-Castellanos, Irene, Azami-Conesa, Iris, Alonso Fernández, Sara, Martínez-Díaz, Rafael A., Navarro-Rocha, Juliana, Gómez-Muñoz, María Teresa, González-Coloma, Azucena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500544/
https://www.ncbi.nlm.nih.gov/pubmed/36157195
http://dx.doi.org/10.3389/fvets.2022.981763
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author Bailén, María
Díaz-Castellanos, Irene
Azami-Conesa, Iris
Alonso Fernández, Sara
Martínez-Díaz, Rafael A.
Navarro-Rocha, Juliana
Gómez-Muñoz, María Teresa
González-Coloma, Azucena
author_facet Bailén, María
Díaz-Castellanos, Irene
Azami-Conesa, Iris
Alonso Fernández, Sara
Martínez-Díaz, Rafael A.
Navarro-Rocha, Juliana
Gómez-Muñoz, María Teresa
González-Coloma, Azucena
author_sort Bailén, María
collection PubMed
description Trichomonas gallinae is a flagellated protozoan that parasitizes the upper digestive tract of various bird species and causes avian trichomonosis. The emergence of resistant strains to the standard treatment, based on nitroimidazoles, increases the need to find alternative therapies. In this study, 36 essential oils (EOs) from Lamiaceae and Asteraceae plant families were tested against T. gallinae trophozoites using the 3-(4,5-dimethylthiazol-2-yl-)-2,5-dipheniltetrazolium bromide (MTT) reduction assay. Among them, EOs from distinct species of Lamiaceae, including the genera Lavandula, Salvia, Thymus, Origanum, and Satureja were the ones reporting better anti-trichomonal activity, and were selected for further analysis, including chemical composition and in vitro assays. The chemical composition of the selected EOs was determined by gas chromatography followed by mass spectrometry and 19 pure compounds were tested against the protozoa, according to their higher abundance in the active EOs. Pure compounds which displayed the highest activity against T. gallinae trophozoites, ordered by highest to lowest activity, were α and β-thujones, camphene, β-pinene, linalyl acetate, thymol, 4-terpineol, γ-terpinene, α-pinene, p-cymene, D-fenchone and β-caryophyllene. A dose dependent effect was observed in most of the EOs and pure compounds tested. The toxicity test conducted in eukaryotic cell cultures with the anti-trichomonal active pure compounds showed that β-caryophyllene, camphene, α-pinene, and β-pinene were slightly toxic for Vero cells, and the selectivity index was calculated. Based on the anti-trichomonal activity and the absence of cytotoxicity results, natural products from Lamiaceae plants could be useful as alternative therapy against avian trichomonosis, mainly those containing linalyl acetate, thymol, 4-terpinenol, γ-terpinene, p-cymene and D-fenchone.
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spelling pubmed-95005442022-09-24 Anti-Trichomonas gallinae activity of essential oils and main compounds from Lamiaceae and Asteraceae plants Bailén, María Díaz-Castellanos, Irene Azami-Conesa, Iris Alonso Fernández, Sara Martínez-Díaz, Rafael A. Navarro-Rocha, Juliana Gómez-Muñoz, María Teresa González-Coloma, Azucena Front Vet Sci Veterinary Science Trichomonas gallinae is a flagellated protozoan that parasitizes the upper digestive tract of various bird species and causes avian trichomonosis. The emergence of resistant strains to the standard treatment, based on nitroimidazoles, increases the need to find alternative therapies. In this study, 36 essential oils (EOs) from Lamiaceae and Asteraceae plant families were tested against T. gallinae trophozoites using the 3-(4,5-dimethylthiazol-2-yl-)-2,5-dipheniltetrazolium bromide (MTT) reduction assay. Among them, EOs from distinct species of Lamiaceae, including the genera Lavandula, Salvia, Thymus, Origanum, and Satureja were the ones reporting better anti-trichomonal activity, and were selected for further analysis, including chemical composition and in vitro assays. The chemical composition of the selected EOs was determined by gas chromatography followed by mass spectrometry and 19 pure compounds were tested against the protozoa, according to their higher abundance in the active EOs. Pure compounds which displayed the highest activity against T. gallinae trophozoites, ordered by highest to lowest activity, were α and β-thujones, camphene, β-pinene, linalyl acetate, thymol, 4-terpineol, γ-terpinene, α-pinene, p-cymene, D-fenchone and β-caryophyllene. A dose dependent effect was observed in most of the EOs and pure compounds tested. The toxicity test conducted in eukaryotic cell cultures with the anti-trichomonal active pure compounds showed that β-caryophyllene, camphene, α-pinene, and β-pinene were slightly toxic for Vero cells, and the selectivity index was calculated. Based on the anti-trichomonal activity and the absence of cytotoxicity results, natural products from Lamiaceae plants could be useful as alternative therapy against avian trichomonosis, mainly those containing linalyl acetate, thymol, 4-terpinenol, γ-terpinene, p-cymene and D-fenchone. Frontiers Media S.A. 2022-09-09 /pmc/articles/PMC9500544/ /pubmed/36157195 http://dx.doi.org/10.3389/fvets.2022.981763 Text en Copyright © 2022 Bailén, Díaz-Castellanos, Azami-Conesa, Alonso Fernández, Martínez-Díaz, Navarro-Rocha, Gómez-Muñoz and González-Coloma. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Bailén, María
Díaz-Castellanos, Irene
Azami-Conesa, Iris
Alonso Fernández, Sara
Martínez-Díaz, Rafael A.
Navarro-Rocha, Juliana
Gómez-Muñoz, María Teresa
González-Coloma, Azucena
Anti-Trichomonas gallinae activity of essential oils and main compounds from Lamiaceae and Asteraceae plants
title Anti-Trichomonas gallinae activity of essential oils and main compounds from Lamiaceae and Asteraceae plants
title_full Anti-Trichomonas gallinae activity of essential oils and main compounds from Lamiaceae and Asteraceae plants
title_fullStr Anti-Trichomonas gallinae activity of essential oils and main compounds from Lamiaceae and Asteraceae plants
title_full_unstemmed Anti-Trichomonas gallinae activity of essential oils and main compounds from Lamiaceae and Asteraceae plants
title_short Anti-Trichomonas gallinae activity of essential oils and main compounds from Lamiaceae and Asteraceae plants
title_sort anti-trichomonas gallinae activity of essential oils and main compounds from lamiaceae and asteraceae plants
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500544/
https://www.ncbi.nlm.nih.gov/pubmed/36157195
http://dx.doi.org/10.3389/fvets.2022.981763
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