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Beneficial effects of whey protein peptides on muscle loss in aging mice models

Aging-related muscle loss is a hallmark of aging and is the cause of some negative outcomes. An optimized diet and supplements have a positive effect in slowing down the process of muscle loss. D-galactose(d-gal) has been used widely to develop aging model. This study explored the beneficial effects...

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Autores principales: Wu, Xin, Yu, Xiaochen, Zhu, Na, Xu, Meihong, Li, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500585/
https://www.ncbi.nlm.nih.gov/pubmed/36159451
http://dx.doi.org/10.3389/fnut.2022.897821
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author Wu, Xin
Yu, Xiaochen
Zhu, Na
Xu, Meihong
Li, Yong
author_facet Wu, Xin
Yu, Xiaochen
Zhu, Na
Xu, Meihong
Li, Yong
author_sort Wu, Xin
collection PubMed
description Aging-related muscle loss is a hallmark of aging and is the cause of some negative outcomes. An optimized diet and supplements have a positive effect in slowing down the process of muscle loss. D-galactose(d-gal) has been used widely to develop aging model. This study explored the beneficial effects of whey protein peptides (WPPs) on sarcopenia in d-gal-induced aging mice. A total of 72 SPF male C57BL/6N mice were used in this study. Sixty mice were modeled by injected intraperitoneally with d-gal (100 mg/kg body weight for 6 weeks), and the other 12 mice were used as control, and injected with the same amount of normal saline. After 6 weeks, the modeled mice were randomly divided into the model control group, whey protein group (1.5 g/kg(*)bw), and three WPPs intervention groups (0.3 g/kg(*)bw, 1.5 g/kg(*)bw, 3.0 g/kg(*)bw), according to serum malondialdehyde (MDA) level. The test samples were orally given to mice by daily garaged. During the 30 days intervention period, the model control group, whey protein group, and WPPs group continued receiving intraperitoneal injections of d-gal, whereas the control group continued receiving intraperitoneal injections of normal saline. The results showed that WPPs could significantly improve the grip strength of aged mice. WPPs could significantly increase lean mass of aged mice and increase muscle weight of gastrocnemius and extensor digitorum longus. WPPs could significantly increase the level of insulin-like growth factor-1 (IGF-1) and reduce level of interleukin (IL)-1, IL-6, tumor necrosis factor-alpha (TNF-α) in serum. WPPs could affect the muscle fiber size in d-gal-induced aging mice. Its specific mechanism may be related to the activation of IGF-1/Akt/mTOR protein synthesis signaling pathway and reduction of the level of inflammation. These results indicate that WPPs can improve aging-related sarcopenia. Compared with whey protein, WPPs supplement seems a better form for sarcopenia.
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spelling pubmed-95005852022-09-24 Beneficial effects of whey protein peptides on muscle loss in aging mice models Wu, Xin Yu, Xiaochen Zhu, Na Xu, Meihong Li, Yong Front Nutr Nutrition Aging-related muscle loss is a hallmark of aging and is the cause of some negative outcomes. An optimized diet and supplements have a positive effect in slowing down the process of muscle loss. D-galactose(d-gal) has been used widely to develop aging model. This study explored the beneficial effects of whey protein peptides (WPPs) on sarcopenia in d-gal-induced aging mice. A total of 72 SPF male C57BL/6N mice were used in this study. Sixty mice were modeled by injected intraperitoneally with d-gal (100 mg/kg body weight for 6 weeks), and the other 12 mice were used as control, and injected with the same amount of normal saline. After 6 weeks, the modeled mice were randomly divided into the model control group, whey protein group (1.5 g/kg(*)bw), and three WPPs intervention groups (0.3 g/kg(*)bw, 1.5 g/kg(*)bw, 3.0 g/kg(*)bw), according to serum malondialdehyde (MDA) level. The test samples were orally given to mice by daily garaged. During the 30 days intervention period, the model control group, whey protein group, and WPPs group continued receiving intraperitoneal injections of d-gal, whereas the control group continued receiving intraperitoneal injections of normal saline. The results showed that WPPs could significantly improve the grip strength of aged mice. WPPs could significantly increase lean mass of aged mice and increase muscle weight of gastrocnemius and extensor digitorum longus. WPPs could significantly increase the level of insulin-like growth factor-1 (IGF-1) and reduce level of interleukin (IL)-1, IL-6, tumor necrosis factor-alpha (TNF-α) in serum. WPPs could affect the muscle fiber size in d-gal-induced aging mice. Its specific mechanism may be related to the activation of IGF-1/Akt/mTOR protein synthesis signaling pathway and reduction of the level of inflammation. These results indicate that WPPs can improve aging-related sarcopenia. Compared with whey protein, WPPs supplement seems a better form for sarcopenia. Frontiers Media S.A. 2022-09-09 /pmc/articles/PMC9500585/ /pubmed/36159451 http://dx.doi.org/10.3389/fnut.2022.897821 Text en Copyright © 2022 Wu, Yu, Zhu, Xu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Wu, Xin
Yu, Xiaochen
Zhu, Na
Xu, Meihong
Li, Yong
Beneficial effects of whey protein peptides on muscle loss in aging mice models
title Beneficial effects of whey protein peptides on muscle loss in aging mice models
title_full Beneficial effects of whey protein peptides on muscle loss in aging mice models
title_fullStr Beneficial effects of whey protein peptides on muscle loss in aging mice models
title_full_unstemmed Beneficial effects of whey protein peptides on muscle loss in aging mice models
title_short Beneficial effects of whey protein peptides on muscle loss in aging mice models
title_sort beneficial effects of whey protein peptides on muscle loss in aging mice models
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500585/
https://www.ncbi.nlm.nih.gov/pubmed/36159451
http://dx.doi.org/10.3389/fnut.2022.897821
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