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Scattered Tubular Cells Markers in Macula Densa of Normal Human Adult Kidney

Background: The scattered tubular cells (STCs) are a population of resident progenitor tubular cells with expansion, self-renewal and epithelial differentiation abilities. Although these cells are localized within the proximal (PTs) and distal (DTs) tubules in a normal adult kidney, their presence h...

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Autores principales: Tossetta, Giovanni, Fantone, Sonia, Lorenzi, Teresa, Galosi, Andrea Benedetto, Sagrati, Andrea, Fabri, Mara, Marzioni, Daniela, Morroni, Manrico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500602/
https://www.ncbi.nlm.nih.gov/pubmed/36142420
http://dx.doi.org/10.3390/ijms231810504
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author Tossetta, Giovanni
Fantone, Sonia
Lorenzi, Teresa
Galosi, Andrea Benedetto
Sagrati, Andrea
Fabri, Mara
Marzioni, Daniela
Morroni, Manrico
author_facet Tossetta, Giovanni
Fantone, Sonia
Lorenzi, Teresa
Galosi, Andrea Benedetto
Sagrati, Andrea
Fabri, Mara
Marzioni, Daniela
Morroni, Manrico
author_sort Tossetta, Giovanni
collection PubMed
description Background: The scattered tubular cells (STCs) are a population of resident progenitor tubular cells with expansion, self-renewal and epithelial differentiation abilities. Although these cells are localized within the proximal (PTs) and distal (DTs) tubules in a normal adult kidney, their presence has never been demonstrated in human macula densa (MD). The purpose of the present study is to describe the presence of STCs in MD using specific markers such as prominin-1 (CD133), cytokeratin 7 (KRT7) and vimentin (VIM). Methods: We analyzed two sets of three consecutive serial sections for each sample. The first sections of each set were immunostained for nNOS to identify MD, the second sections were immune-stained for CD133 (specific STCs marker) while the third sections were analyzed for KRT7 (another STCs specific marker) and VIM (that stains the basal pole of the STCs) in the first and second sets, respectively, in order to study the co-expression of KRT7 and VIM with the CD133 marker. Results: CD133 was localized in some MD cells and in the adjacent DT cells. Moreover, CD133 was detected in the parietal epithelial cells of Bowman’s capsule and in some proximal tubules (PT). KRT7-positive cells were identified in MD and adjacent DT cells, while KRT7 positivity was mostly confined in both DT and collecting ducts (CD) in the other areas of the renal parenchyma. CD133 and KRT7 were co-expressed in some MD and adjacent DT cells. Some of the latter cells were positive both for CD133 and VIM. CD133 was always localized in the apical part of the cells, whereas the VIM expression was evident only in the cellular basal pole. Although some cells of MD expressed VIM or CD133, none of them co-expressed VIM and CD133. Conclusions: The presence of STCs was demonstrated in human adult MD, suggesting that this structure has expansion, self-renewal and epithelial differentiation abilities, similar to all other parts of renal tubules.
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spelling pubmed-95006022022-09-24 Scattered Tubular Cells Markers in Macula Densa of Normal Human Adult Kidney Tossetta, Giovanni Fantone, Sonia Lorenzi, Teresa Galosi, Andrea Benedetto Sagrati, Andrea Fabri, Mara Marzioni, Daniela Morroni, Manrico Int J Mol Sci Article Background: The scattered tubular cells (STCs) are a population of resident progenitor tubular cells with expansion, self-renewal and epithelial differentiation abilities. Although these cells are localized within the proximal (PTs) and distal (DTs) tubules in a normal adult kidney, their presence has never been demonstrated in human macula densa (MD). The purpose of the present study is to describe the presence of STCs in MD using specific markers such as prominin-1 (CD133), cytokeratin 7 (KRT7) and vimentin (VIM). Methods: We analyzed two sets of three consecutive serial sections for each sample. The first sections of each set were immunostained for nNOS to identify MD, the second sections were immune-stained for CD133 (specific STCs marker) while the third sections were analyzed for KRT7 (another STCs specific marker) and VIM (that stains the basal pole of the STCs) in the first and second sets, respectively, in order to study the co-expression of KRT7 and VIM with the CD133 marker. Results: CD133 was localized in some MD cells and in the adjacent DT cells. Moreover, CD133 was detected in the parietal epithelial cells of Bowman’s capsule and in some proximal tubules (PT). KRT7-positive cells were identified in MD and adjacent DT cells, while KRT7 positivity was mostly confined in both DT and collecting ducts (CD) in the other areas of the renal parenchyma. CD133 and KRT7 were co-expressed in some MD and adjacent DT cells. Some of the latter cells were positive both for CD133 and VIM. CD133 was always localized in the apical part of the cells, whereas the VIM expression was evident only in the cellular basal pole. Although some cells of MD expressed VIM or CD133, none of them co-expressed VIM and CD133. Conclusions: The presence of STCs was demonstrated in human adult MD, suggesting that this structure has expansion, self-renewal and epithelial differentiation abilities, similar to all other parts of renal tubules. MDPI 2022-09-10 /pmc/articles/PMC9500602/ /pubmed/36142420 http://dx.doi.org/10.3390/ijms231810504 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tossetta, Giovanni
Fantone, Sonia
Lorenzi, Teresa
Galosi, Andrea Benedetto
Sagrati, Andrea
Fabri, Mara
Marzioni, Daniela
Morroni, Manrico
Scattered Tubular Cells Markers in Macula Densa of Normal Human Adult Kidney
title Scattered Tubular Cells Markers in Macula Densa of Normal Human Adult Kidney
title_full Scattered Tubular Cells Markers in Macula Densa of Normal Human Adult Kidney
title_fullStr Scattered Tubular Cells Markers in Macula Densa of Normal Human Adult Kidney
title_full_unstemmed Scattered Tubular Cells Markers in Macula Densa of Normal Human Adult Kidney
title_short Scattered Tubular Cells Markers in Macula Densa of Normal Human Adult Kidney
title_sort scattered tubular cells markers in macula densa of normal human adult kidney
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500602/
https://www.ncbi.nlm.nih.gov/pubmed/36142420
http://dx.doi.org/10.3390/ijms231810504
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