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Arabic Gum Could Alleviate the Aflatoxin B(1)-provoked Hepatic Injury in Rat: The Involvement of Oxidative Stress, Inflammatory, and Apoptotic Pathways

Aflatoxin B(1) (AF) is an unavoidable environmental pollutant that contaminates food, feed, and grains, which seriously threatens human and animal health. Arabic gum (AG) has recently evoked much attention owing to its promising therapeutic potential. Thus, the current study was conducted to look in...

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Autores principales: Ahmed, Noha, El-Rayes, Samir M., Khalil, Waleed F., Abdeen, Ahmed, Abdelkader, Afaf, Youssef, Mohammed, Maher, Zainab M., Ibrahim, Amany N., Abdelrahman, Shaymaa M., Ibrahim, Samah F., Abdelrahaman, Doaa, Alsieni, Mohammed, Elserafy, Osama S., Ghamry, Heba I., Emam, Hanan T., Shanab, Obeid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500620/
https://www.ncbi.nlm.nih.gov/pubmed/36136543
http://dx.doi.org/10.3390/toxins14090605
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author Ahmed, Noha
El-Rayes, Samir M.
Khalil, Waleed F.
Abdeen, Ahmed
Abdelkader, Afaf
Youssef, Mohammed
Maher, Zainab M.
Ibrahim, Amany N.
Abdelrahman, Shaymaa M.
Ibrahim, Samah F.
Abdelrahaman, Doaa
Alsieni, Mohammed
Elserafy, Osama S.
Ghamry, Heba I.
Emam, Hanan T.
Shanab, Obeid
author_facet Ahmed, Noha
El-Rayes, Samir M.
Khalil, Waleed F.
Abdeen, Ahmed
Abdelkader, Afaf
Youssef, Mohammed
Maher, Zainab M.
Ibrahim, Amany N.
Abdelrahman, Shaymaa M.
Ibrahim, Samah F.
Abdelrahaman, Doaa
Alsieni, Mohammed
Elserafy, Osama S.
Ghamry, Heba I.
Emam, Hanan T.
Shanab, Obeid
author_sort Ahmed, Noha
collection PubMed
description Aflatoxin B(1) (AF) is an unavoidable environmental pollutant that contaminates food, feed, and grains, which seriously threatens human and animal health. Arabic gum (AG) has recently evoked much attention owing to its promising therapeutic potential. Thus, the current study was conducted to look into the possible mechanisms beyond the ameliorative activity of AG against AF-inflicted hepatic injury. Male Wistar rats were assigned into four groups: Control, AG (7.5 g/kg b.w/day, orally), AF (200 µg/kg b.w), and AG plus AF group. AF induced marked liver damage expounded by considerable changes in biochemical profile and histological architecture. The oxidative stress stimulated by AF boosted the production of plasma malondialdehyde (MDA) level along with decreases in the total antioxidant capacity (TAC) level and glutathione peroxidase (GPx) activity. Additionally, AF exposure was associated with down-regulation of the nuclear factor erythroid2–related factor2 (Nrf2) and superoxide dismutase1 (SOD1) protein expression in liver tissue. Apoptotic cascade has also been evoked following AF-exposure, as depicted in overexpression of cytochrome c (Cyto c), cleaved Caspase3 (Cl. Casp3), along with enhanced up-regulation of inflammatory mediators such as tumor necrosis factor-α (TNF-α), interleukin (IL)-6, inducible nitric oxide synthase (iNOS), and nuclear factor kappa-B transcription factor/p65 (NF-κB/p65) mRNA expression levels. Interestingly, the antioxidant and anti-inflammatory contents of AG may reverse the induced oxidative damage, inflammation, and apoptosis in AF-exposed animals.
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spelling pubmed-95006202022-09-24 Arabic Gum Could Alleviate the Aflatoxin B(1)-provoked Hepatic Injury in Rat: The Involvement of Oxidative Stress, Inflammatory, and Apoptotic Pathways Ahmed, Noha El-Rayes, Samir M. Khalil, Waleed F. Abdeen, Ahmed Abdelkader, Afaf Youssef, Mohammed Maher, Zainab M. Ibrahim, Amany N. Abdelrahman, Shaymaa M. Ibrahim, Samah F. Abdelrahaman, Doaa Alsieni, Mohammed Elserafy, Osama S. Ghamry, Heba I. Emam, Hanan T. Shanab, Obeid Toxins (Basel) Article Aflatoxin B(1) (AF) is an unavoidable environmental pollutant that contaminates food, feed, and grains, which seriously threatens human and animal health. Arabic gum (AG) has recently evoked much attention owing to its promising therapeutic potential. Thus, the current study was conducted to look into the possible mechanisms beyond the ameliorative activity of AG against AF-inflicted hepatic injury. Male Wistar rats were assigned into four groups: Control, AG (7.5 g/kg b.w/day, orally), AF (200 µg/kg b.w), and AG plus AF group. AF induced marked liver damage expounded by considerable changes in biochemical profile and histological architecture. The oxidative stress stimulated by AF boosted the production of plasma malondialdehyde (MDA) level along with decreases in the total antioxidant capacity (TAC) level and glutathione peroxidase (GPx) activity. Additionally, AF exposure was associated with down-regulation of the nuclear factor erythroid2–related factor2 (Nrf2) and superoxide dismutase1 (SOD1) protein expression in liver tissue. Apoptotic cascade has also been evoked following AF-exposure, as depicted in overexpression of cytochrome c (Cyto c), cleaved Caspase3 (Cl. Casp3), along with enhanced up-regulation of inflammatory mediators such as tumor necrosis factor-α (TNF-α), interleukin (IL)-6, inducible nitric oxide synthase (iNOS), and nuclear factor kappa-B transcription factor/p65 (NF-κB/p65) mRNA expression levels. Interestingly, the antioxidant and anti-inflammatory contents of AG may reverse the induced oxidative damage, inflammation, and apoptosis in AF-exposed animals. MDPI 2022-09-01 /pmc/articles/PMC9500620/ /pubmed/36136543 http://dx.doi.org/10.3390/toxins14090605 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ahmed, Noha
El-Rayes, Samir M.
Khalil, Waleed F.
Abdeen, Ahmed
Abdelkader, Afaf
Youssef, Mohammed
Maher, Zainab M.
Ibrahim, Amany N.
Abdelrahman, Shaymaa M.
Ibrahim, Samah F.
Abdelrahaman, Doaa
Alsieni, Mohammed
Elserafy, Osama S.
Ghamry, Heba I.
Emam, Hanan T.
Shanab, Obeid
Arabic Gum Could Alleviate the Aflatoxin B(1)-provoked Hepatic Injury in Rat: The Involvement of Oxidative Stress, Inflammatory, and Apoptotic Pathways
title Arabic Gum Could Alleviate the Aflatoxin B(1)-provoked Hepatic Injury in Rat: The Involvement of Oxidative Stress, Inflammatory, and Apoptotic Pathways
title_full Arabic Gum Could Alleviate the Aflatoxin B(1)-provoked Hepatic Injury in Rat: The Involvement of Oxidative Stress, Inflammatory, and Apoptotic Pathways
title_fullStr Arabic Gum Could Alleviate the Aflatoxin B(1)-provoked Hepatic Injury in Rat: The Involvement of Oxidative Stress, Inflammatory, and Apoptotic Pathways
title_full_unstemmed Arabic Gum Could Alleviate the Aflatoxin B(1)-provoked Hepatic Injury in Rat: The Involvement of Oxidative Stress, Inflammatory, and Apoptotic Pathways
title_short Arabic Gum Could Alleviate the Aflatoxin B(1)-provoked Hepatic Injury in Rat: The Involvement of Oxidative Stress, Inflammatory, and Apoptotic Pathways
title_sort arabic gum could alleviate the aflatoxin b(1)-provoked hepatic injury in rat: the involvement of oxidative stress, inflammatory, and apoptotic pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500620/
https://www.ncbi.nlm.nih.gov/pubmed/36136543
http://dx.doi.org/10.3390/toxins14090605
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