Cargando…
Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species
Citronella mucronata (C. mucronata), a tree species endemic to Chile, has become threatened in its natural habitat and is currently listed as vulnerable. Tree population parameters have deteriorated due to indiscriminate logging and other anthropogenic activities, warranting research on mass propaga...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500790/ https://www.ncbi.nlm.nih.gov/pubmed/36145826 http://dx.doi.org/10.3390/plants11182425 |
_version_ | 1784795309064323072 |
---|---|
author | Guerra, Francesca Badilla, Loreto Cautin, Ricardo Castro, Mónica |
author_facet | Guerra, Francesca Badilla, Loreto Cautin, Ricardo Castro, Mónica |
author_sort | Guerra, Francesca |
collection | PubMed |
description | Citronella mucronata (C. mucronata), a tree species endemic to Chile, has become threatened in its natural habitat and is currently listed as vulnerable. Tree population parameters have deteriorated due to indiscriminate logging and other anthropogenic activities, warranting research on mass propagation as a means of recovery. This study, unprecedented for this native species, has developed a successful method for its micropropagation. The objective was to establish a protocol for in vitro propagation of C. mucronata to produce large quantities of high-quality seedlings in an accelerated plant acquisition process. The best results were achieved by growing explants on Murashige and Skoog (MS) basal culture medium supplemented with 4.44 μM 6-benzylaminopurine (BAP) and 14.76 μM indole-3-butyric acid (IBA). Explant survival rate was 78 %, the average shoot length reached 3.2 cm, the number of lateral shoots was 3.9, and rooting rate was 60%. Furthermore, stimulation with red and blue light in a 1:2 ratio, supplemented with 14.76 μM IBA, improved the rooting rate to 93%. The survival rate of rooted explants reached 100% in the acclimatization stage when using peat and perlite substrate (1:1 v/v). |
format | Online Article Text |
id | pubmed-9500790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95007902022-09-24 Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species Guerra, Francesca Badilla, Loreto Cautin, Ricardo Castro, Mónica Plants (Basel) Article Citronella mucronata (C. mucronata), a tree species endemic to Chile, has become threatened in its natural habitat and is currently listed as vulnerable. Tree population parameters have deteriorated due to indiscriminate logging and other anthropogenic activities, warranting research on mass propagation as a means of recovery. This study, unprecedented for this native species, has developed a successful method for its micropropagation. The objective was to establish a protocol for in vitro propagation of C. mucronata to produce large quantities of high-quality seedlings in an accelerated plant acquisition process. The best results were achieved by growing explants on Murashige and Skoog (MS) basal culture medium supplemented with 4.44 μM 6-benzylaminopurine (BAP) and 14.76 μM indole-3-butyric acid (IBA). Explant survival rate was 78 %, the average shoot length reached 3.2 cm, the number of lateral shoots was 3.9, and rooting rate was 60%. Furthermore, stimulation with red and blue light in a 1:2 ratio, supplemented with 14.76 μM IBA, improved the rooting rate to 93%. The survival rate of rooted explants reached 100% in the acclimatization stage when using peat and perlite substrate (1:1 v/v). MDPI 2022-09-16 /pmc/articles/PMC9500790/ /pubmed/36145826 http://dx.doi.org/10.3390/plants11182425 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guerra, Francesca Badilla, Loreto Cautin, Ricardo Castro, Mónica Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species |
title | Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species |
title_full | Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species |
title_fullStr | Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species |
title_full_unstemmed | Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species |
title_short | Micropropagation of Citronella mucronata D. Don, a Vulnerable Chilean Endemic Tree Species |
title_sort | micropropagation of citronella mucronata d. don, a vulnerable chilean endemic tree species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9500790/ https://www.ncbi.nlm.nih.gov/pubmed/36145826 http://dx.doi.org/10.3390/plants11182425 |
work_keys_str_mv | AT guerrafrancesca micropropagationofcitronellamucronataddonavulnerablechileanendemictreespecies AT badillaloreto micropropagationofcitronellamucronataddonavulnerablechileanendemictreespecies AT cautinricardo micropropagationofcitronellamucronataddonavulnerablechileanendemictreespecies AT castromonica micropropagationofcitronellamucronataddonavulnerablechileanendemictreespecies |