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Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens

An ideal vaccine for controlling Salmonella infection in chicken flocks should be safe, inducing both humoral and cellular immunity. Live attenuated vaccines against Salmonella Enteritidis (S. Enteritidis) have been used as a potential control method of Salmonella infection in the poultry industry....

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Autores principales: Shin, Hyunjin, La, Tae-Min, Lee, Hong-Jae, Kim, Taesoo, Song, Seung-un, Park, Eunjin, Park, Gyu-Hyung, Choi, In-Soo, Park, Seung-Yong, Lee, Joong-Bok, Lee, Sang-Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501076/
https://www.ncbi.nlm.nih.gov/pubmed/36146484
http://dx.doi.org/10.3390/vaccines10091405
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author Shin, Hyunjin
La, Tae-Min
Lee, Hong-Jae
Kim, Taesoo
Song, Seung-un
Park, Eunjin
Park, Gyu-Hyung
Choi, In-Soo
Park, Seung-Yong
Lee, Joong-Bok
Lee, Sang-Won
author_facet Shin, Hyunjin
La, Tae-Min
Lee, Hong-Jae
Kim, Taesoo
Song, Seung-un
Park, Eunjin
Park, Gyu-Hyung
Choi, In-Soo
Park, Seung-Yong
Lee, Joong-Bok
Lee, Sang-Won
author_sort Shin, Hyunjin
collection PubMed
description An ideal vaccine for controlling Salmonella infection in chicken flocks should be safe, inducing both humoral and cellular immunity. Live attenuated vaccines against Salmonella Enteritidis (S. Enteritidis) have been used as a potential control method of Salmonella infection in the poultry industry. However, live attenuated vaccines can persistently infect poultry for long periods and can become virulent revertant strains. In this study, we assessed the immune responses and protective efficacy of a temperature-sensitive attenuated S. Enteritidis mutant as a potential vaccine candidate. In addition, we evaluated the combined vaccine administration methods to maximize both humoral and cellular immune responses in chickens induced by the vaccine candidate. Immune responses and protective efficacy were compared between the Oral/IM group, vaccinated using one oral dose at four weeks old and a booster intramuscular dose at seven weeks old, and the IM/Oral group, vaccinated using one intramuscular dose at four weeks old and a booster oral dose at seven weeks old. The Oral/IM group showed stronger immune responses than those of the IM/Oral group. Spleens from the Oral/IM group showed a promising tendency of reduction of challenged Salmonella compared with those of other groups. Overall, the results indicated that the S. Enteritidis mutant strain is a promising live attenuated vaccine candidate with good efficacy.
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spelling pubmed-95010762022-09-24 Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens Shin, Hyunjin La, Tae-Min Lee, Hong-Jae Kim, Taesoo Song, Seung-un Park, Eunjin Park, Gyu-Hyung Choi, In-Soo Park, Seung-Yong Lee, Joong-Bok Lee, Sang-Won Vaccines (Basel) Article An ideal vaccine for controlling Salmonella infection in chicken flocks should be safe, inducing both humoral and cellular immunity. Live attenuated vaccines against Salmonella Enteritidis (S. Enteritidis) have been used as a potential control method of Salmonella infection in the poultry industry. However, live attenuated vaccines can persistently infect poultry for long periods and can become virulent revertant strains. In this study, we assessed the immune responses and protective efficacy of a temperature-sensitive attenuated S. Enteritidis mutant as a potential vaccine candidate. In addition, we evaluated the combined vaccine administration methods to maximize both humoral and cellular immune responses in chickens induced by the vaccine candidate. Immune responses and protective efficacy were compared between the Oral/IM group, vaccinated using one oral dose at four weeks old and a booster intramuscular dose at seven weeks old, and the IM/Oral group, vaccinated using one intramuscular dose at four weeks old and a booster oral dose at seven weeks old. The Oral/IM group showed stronger immune responses than those of the IM/Oral group. Spleens from the Oral/IM group showed a promising tendency of reduction of challenged Salmonella compared with those of other groups. Overall, the results indicated that the S. Enteritidis mutant strain is a promising live attenuated vaccine candidate with good efficacy. MDPI 2022-08-27 /pmc/articles/PMC9501076/ /pubmed/36146484 http://dx.doi.org/10.3390/vaccines10091405 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shin, Hyunjin
La, Tae-Min
Lee, Hong-Jae
Kim, Taesoo
Song, Seung-un
Park, Eunjin
Park, Gyu-Hyung
Choi, In-Soo
Park, Seung-Yong
Lee, Joong-Bok
Lee, Sang-Won
Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens
title Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens
title_full Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens
title_fullStr Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens
title_full_unstemmed Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens
title_short Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens
title_sort evaluation of immune responses and protective efficacy of a novel live attenuated salmonella enteritidis vaccine candidate in chickens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501076/
https://www.ncbi.nlm.nih.gov/pubmed/36146484
http://dx.doi.org/10.3390/vaccines10091405
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