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Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens
An ideal vaccine for controlling Salmonella infection in chicken flocks should be safe, inducing both humoral and cellular immunity. Live attenuated vaccines against Salmonella Enteritidis (S. Enteritidis) have been used as a potential control method of Salmonella infection in the poultry industry....
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501076/ https://www.ncbi.nlm.nih.gov/pubmed/36146484 http://dx.doi.org/10.3390/vaccines10091405 |
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author | Shin, Hyunjin La, Tae-Min Lee, Hong-Jae Kim, Taesoo Song, Seung-un Park, Eunjin Park, Gyu-Hyung Choi, In-Soo Park, Seung-Yong Lee, Joong-Bok Lee, Sang-Won |
author_facet | Shin, Hyunjin La, Tae-Min Lee, Hong-Jae Kim, Taesoo Song, Seung-un Park, Eunjin Park, Gyu-Hyung Choi, In-Soo Park, Seung-Yong Lee, Joong-Bok Lee, Sang-Won |
author_sort | Shin, Hyunjin |
collection | PubMed |
description | An ideal vaccine for controlling Salmonella infection in chicken flocks should be safe, inducing both humoral and cellular immunity. Live attenuated vaccines against Salmonella Enteritidis (S. Enteritidis) have been used as a potential control method of Salmonella infection in the poultry industry. However, live attenuated vaccines can persistently infect poultry for long periods and can become virulent revertant strains. In this study, we assessed the immune responses and protective efficacy of a temperature-sensitive attenuated S. Enteritidis mutant as a potential vaccine candidate. In addition, we evaluated the combined vaccine administration methods to maximize both humoral and cellular immune responses in chickens induced by the vaccine candidate. Immune responses and protective efficacy were compared between the Oral/IM group, vaccinated using one oral dose at four weeks old and a booster intramuscular dose at seven weeks old, and the IM/Oral group, vaccinated using one intramuscular dose at four weeks old and a booster oral dose at seven weeks old. The Oral/IM group showed stronger immune responses than those of the IM/Oral group. Spleens from the Oral/IM group showed a promising tendency of reduction of challenged Salmonella compared with those of other groups. Overall, the results indicated that the S. Enteritidis mutant strain is a promising live attenuated vaccine candidate with good efficacy. |
format | Online Article Text |
id | pubmed-9501076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95010762022-09-24 Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens Shin, Hyunjin La, Tae-Min Lee, Hong-Jae Kim, Taesoo Song, Seung-un Park, Eunjin Park, Gyu-Hyung Choi, In-Soo Park, Seung-Yong Lee, Joong-Bok Lee, Sang-Won Vaccines (Basel) Article An ideal vaccine for controlling Salmonella infection in chicken flocks should be safe, inducing both humoral and cellular immunity. Live attenuated vaccines against Salmonella Enteritidis (S. Enteritidis) have been used as a potential control method of Salmonella infection in the poultry industry. However, live attenuated vaccines can persistently infect poultry for long periods and can become virulent revertant strains. In this study, we assessed the immune responses and protective efficacy of a temperature-sensitive attenuated S. Enteritidis mutant as a potential vaccine candidate. In addition, we evaluated the combined vaccine administration methods to maximize both humoral and cellular immune responses in chickens induced by the vaccine candidate. Immune responses and protective efficacy were compared between the Oral/IM group, vaccinated using one oral dose at four weeks old and a booster intramuscular dose at seven weeks old, and the IM/Oral group, vaccinated using one intramuscular dose at four weeks old and a booster oral dose at seven weeks old. The Oral/IM group showed stronger immune responses than those of the IM/Oral group. Spleens from the Oral/IM group showed a promising tendency of reduction of challenged Salmonella compared with those of other groups. Overall, the results indicated that the S. Enteritidis mutant strain is a promising live attenuated vaccine candidate with good efficacy. MDPI 2022-08-27 /pmc/articles/PMC9501076/ /pubmed/36146484 http://dx.doi.org/10.3390/vaccines10091405 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shin, Hyunjin La, Tae-Min Lee, Hong-Jae Kim, Taesoo Song, Seung-un Park, Eunjin Park, Gyu-Hyung Choi, In-Soo Park, Seung-Yong Lee, Joong-Bok Lee, Sang-Won Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens |
title | Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens |
title_full | Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens |
title_fullStr | Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens |
title_full_unstemmed | Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens |
title_short | Evaluation of Immune Responses and Protective Efficacy of a Novel Live Attenuated Salmonella Enteritidis Vaccine Candidate in Chickens |
title_sort | evaluation of immune responses and protective efficacy of a novel live attenuated salmonella enteritidis vaccine candidate in chickens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501076/ https://www.ncbi.nlm.nih.gov/pubmed/36146484 http://dx.doi.org/10.3390/vaccines10091405 |
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