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Protection of Erythrocytes and Microvascular Endothelial Cells against Oxidative Damage by Fragaria vesca L. and Rubus idaeus L. Leaves Extracts—The Mechanism of Action

The aim of this work is to determine the biological activity of ellagitannins rich extracts from leaves of raspberry (Rubus idaeus L.) and wild strawberry (Fragaria vesca L.) in relation to cells and cell membranes. Detailed qualitative and quantitative analysis of phenolic compounds of the extract...

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Autores principales: Cyboran-Mikołajczyk, Sylwia, Męczarska, Katarzyna, Solarska-Ściuk, Katarzyna, Ratajczak-Wielgomas, Katarzyna, Oszmiański, Jan, Jencova, Vera, Bonarska-Kujawa, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501125/
https://www.ncbi.nlm.nih.gov/pubmed/36144602
http://dx.doi.org/10.3390/molecules27185865
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author Cyboran-Mikołajczyk, Sylwia
Męczarska, Katarzyna
Solarska-Ściuk, Katarzyna
Ratajczak-Wielgomas, Katarzyna
Oszmiański, Jan
Jencova, Vera
Bonarska-Kujawa, Dorota
author_facet Cyboran-Mikołajczyk, Sylwia
Męczarska, Katarzyna
Solarska-Ściuk, Katarzyna
Ratajczak-Wielgomas, Katarzyna
Oszmiański, Jan
Jencova, Vera
Bonarska-Kujawa, Dorota
author_sort Cyboran-Mikołajczyk, Sylwia
collection PubMed
description The aim of this work is to determine the biological activity of ellagitannins rich extracts from leaves of raspberry (Rubus idaeus L.) and wild strawberry (Fragaria vesca L.) in relation to cells and cell membranes. Detailed qualitative and quantitative analysis of phenolic compounds of the extract was made using chromatographic methods. Cytotoxic and antioxidant activities of tested extracts in relation to erythrocytes and human vascular endothelial cells (HMEC-1) were determined by using fluorimetric and spectrophotometric methods. In order to establish the influence of the extracts on the physical properties of the membrane, such as osmotic resistance and erythrocytes shapes, mobility and/or hydration of polar heads and fluidity of hydrocarbon chains of membrane lipids, microscopic and spectroscopic methods were used. The results showed that the extracts are non-toxic for erythrocytes and HMEC-1 cells (up to concentration of 50 µg/mL), but they effectively protect cells and their membranes against oxidative damage. The increase in osmotic resistance of erythrocytes, formation of echinocytes and changes only in the polar part of the membrane caused by the extracts demonstrate their location mainly in the hydrophilic part of the membrane. The results indicate that tested extracts have high biological activities and may be potentially used in delaying the ageing process of organisms and prevention of many diseases, especially those associated with oxidative stress.
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spelling pubmed-95011252022-09-24 Protection of Erythrocytes and Microvascular Endothelial Cells against Oxidative Damage by Fragaria vesca L. and Rubus idaeus L. Leaves Extracts—The Mechanism of Action Cyboran-Mikołajczyk, Sylwia Męczarska, Katarzyna Solarska-Ściuk, Katarzyna Ratajczak-Wielgomas, Katarzyna Oszmiański, Jan Jencova, Vera Bonarska-Kujawa, Dorota Molecules Article The aim of this work is to determine the biological activity of ellagitannins rich extracts from leaves of raspberry (Rubus idaeus L.) and wild strawberry (Fragaria vesca L.) in relation to cells and cell membranes. Detailed qualitative and quantitative analysis of phenolic compounds of the extract was made using chromatographic methods. Cytotoxic and antioxidant activities of tested extracts in relation to erythrocytes and human vascular endothelial cells (HMEC-1) were determined by using fluorimetric and spectrophotometric methods. In order to establish the influence of the extracts on the physical properties of the membrane, such as osmotic resistance and erythrocytes shapes, mobility and/or hydration of polar heads and fluidity of hydrocarbon chains of membrane lipids, microscopic and spectroscopic methods were used. The results showed that the extracts are non-toxic for erythrocytes and HMEC-1 cells (up to concentration of 50 µg/mL), but they effectively protect cells and their membranes against oxidative damage. The increase in osmotic resistance of erythrocytes, formation of echinocytes and changes only in the polar part of the membrane caused by the extracts demonstrate their location mainly in the hydrophilic part of the membrane. The results indicate that tested extracts have high biological activities and may be potentially used in delaying the ageing process of organisms and prevention of many diseases, especially those associated with oxidative stress. MDPI 2022-09-10 /pmc/articles/PMC9501125/ /pubmed/36144602 http://dx.doi.org/10.3390/molecules27185865 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cyboran-Mikołajczyk, Sylwia
Męczarska, Katarzyna
Solarska-Ściuk, Katarzyna
Ratajczak-Wielgomas, Katarzyna
Oszmiański, Jan
Jencova, Vera
Bonarska-Kujawa, Dorota
Protection of Erythrocytes and Microvascular Endothelial Cells against Oxidative Damage by Fragaria vesca L. and Rubus idaeus L. Leaves Extracts—The Mechanism of Action
title Protection of Erythrocytes and Microvascular Endothelial Cells against Oxidative Damage by Fragaria vesca L. and Rubus idaeus L. Leaves Extracts—The Mechanism of Action
title_full Protection of Erythrocytes and Microvascular Endothelial Cells against Oxidative Damage by Fragaria vesca L. and Rubus idaeus L. Leaves Extracts—The Mechanism of Action
title_fullStr Protection of Erythrocytes and Microvascular Endothelial Cells against Oxidative Damage by Fragaria vesca L. and Rubus idaeus L. Leaves Extracts—The Mechanism of Action
title_full_unstemmed Protection of Erythrocytes and Microvascular Endothelial Cells against Oxidative Damage by Fragaria vesca L. and Rubus idaeus L. Leaves Extracts—The Mechanism of Action
title_short Protection of Erythrocytes and Microvascular Endothelial Cells against Oxidative Damage by Fragaria vesca L. and Rubus idaeus L. Leaves Extracts—The Mechanism of Action
title_sort protection of erythrocytes and microvascular endothelial cells against oxidative damage by fragaria vesca l. and rubus idaeus l. leaves extracts—the mechanism of action
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501125/
https://www.ncbi.nlm.nih.gov/pubmed/36144602
http://dx.doi.org/10.3390/molecules27185865
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