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Amphiphysin AoRvs167-Mediated Membrane Curvature Facilitates Trap Formation, Endocytosis, and Stress Resistance in Arthrobotrys oligospora

Bin1/Amphiphysin/Rvs (BAR) domain-containing proteins mediate fundamental cellular processes, including membrane remodeling and endocytosis. Nematode-trapping (NT) fungi can differentiate to form trapping structures through highly reorganized cell membranes and walls. In this study, we identified th...

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Autores principales: Cui, Peijie, Tian, Mengqing, Huang, Jinrong, Zheng, Xi, Guo, Yingqi, Li, Guohong, Wang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501185/
https://www.ncbi.nlm.nih.gov/pubmed/36145429
http://dx.doi.org/10.3390/pathogens11090997
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author Cui, Peijie
Tian, Mengqing
Huang, Jinrong
Zheng, Xi
Guo, Yingqi
Li, Guohong
Wang, Xin
author_facet Cui, Peijie
Tian, Mengqing
Huang, Jinrong
Zheng, Xi
Guo, Yingqi
Li, Guohong
Wang, Xin
author_sort Cui, Peijie
collection PubMed
description Bin1/Amphiphysin/Rvs (BAR) domain-containing proteins mediate fundamental cellular processes, including membrane remodeling and endocytosis. Nematode-trapping (NT) fungi can differentiate to form trapping structures through highly reorganized cell membranes and walls. In this study, we identified the NT fungus Arthrobotrys oligospora ortholog of yeast Rvs167 and documented its involvement in membrane bending and endocytosis. We further confirmed that the deletion of AoRvs167 makes the fungus more hypersensitive to osmotic salt (Nacl), higher temperatures (28 to 30 °C), and the cell wall perturbation agent Congo red. In addition, the disruption of AoRvs167 reduced the trap formation capacity. Hence, AoRvs167 may regulate fungal pathogenicity through the integrity of plasma membranes and cell walls.
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spelling pubmed-95011852022-09-24 Amphiphysin AoRvs167-Mediated Membrane Curvature Facilitates Trap Formation, Endocytosis, and Stress Resistance in Arthrobotrys oligospora Cui, Peijie Tian, Mengqing Huang, Jinrong Zheng, Xi Guo, Yingqi Li, Guohong Wang, Xin Pathogens Article Bin1/Amphiphysin/Rvs (BAR) domain-containing proteins mediate fundamental cellular processes, including membrane remodeling and endocytosis. Nematode-trapping (NT) fungi can differentiate to form trapping structures through highly reorganized cell membranes and walls. In this study, we identified the NT fungus Arthrobotrys oligospora ortholog of yeast Rvs167 and documented its involvement in membrane bending and endocytosis. We further confirmed that the deletion of AoRvs167 makes the fungus more hypersensitive to osmotic salt (Nacl), higher temperatures (28 to 30 °C), and the cell wall perturbation agent Congo red. In addition, the disruption of AoRvs167 reduced the trap formation capacity. Hence, AoRvs167 may regulate fungal pathogenicity through the integrity of plasma membranes and cell walls. MDPI 2022-08-31 /pmc/articles/PMC9501185/ /pubmed/36145429 http://dx.doi.org/10.3390/pathogens11090997 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cui, Peijie
Tian, Mengqing
Huang, Jinrong
Zheng, Xi
Guo, Yingqi
Li, Guohong
Wang, Xin
Amphiphysin AoRvs167-Mediated Membrane Curvature Facilitates Trap Formation, Endocytosis, and Stress Resistance in Arthrobotrys oligospora
title Amphiphysin AoRvs167-Mediated Membrane Curvature Facilitates Trap Formation, Endocytosis, and Stress Resistance in Arthrobotrys oligospora
title_full Amphiphysin AoRvs167-Mediated Membrane Curvature Facilitates Trap Formation, Endocytosis, and Stress Resistance in Arthrobotrys oligospora
title_fullStr Amphiphysin AoRvs167-Mediated Membrane Curvature Facilitates Trap Formation, Endocytosis, and Stress Resistance in Arthrobotrys oligospora
title_full_unstemmed Amphiphysin AoRvs167-Mediated Membrane Curvature Facilitates Trap Formation, Endocytosis, and Stress Resistance in Arthrobotrys oligospora
title_short Amphiphysin AoRvs167-Mediated Membrane Curvature Facilitates Trap Formation, Endocytosis, and Stress Resistance in Arthrobotrys oligospora
title_sort amphiphysin aorvs167-mediated membrane curvature facilitates trap formation, endocytosis, and stress resistance in arthrobotrys oligospora
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501185/
https://www.ncbi.nlm.nih.gov/pubmed/36145429
http://dx.doi.org/10.3390/pathogens11090997
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