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Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation

Immobilization is a commonly used method in response to the need to increase the resistance of microorganisms to the toxic effects of xenobiotics. In this study, a plant sponge from Luffa cylindrica was used as a carrier for the immobilization of the Stenotrophomonas maltophilia KB2 strain since suc...

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Autores principales: Wojcieszyńska, Danuta, Klamka, Judyta, Marchlewicz, Ariel, Potocka, Izabela, Żur-Pińska, Joanna, Guzik, Urszula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501314/
https://www.ncbi.nlm.nih.gov/pubmed/36144528
http://dx.doi.org/10.3390/molecules27185795
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author Wojcieszyńska, Danuta
Klamka, Judyta
Marchlewicz, Ariel
Potocka, Izabela
Żur-Pińska, Joanna
Guzik, Urszula
author_facet Wojcieszyńska, Danuta
Klamka, Judyta
Marchlewicz, Ariel
Potocka, Izabela
Żur-Pińska, Joanna
Guzik, Urszula
author_sort Wojcieszyńska, Danuta
collection PubMed
description Immobilization is a commonly used method in response to the need to increase the resistance of microorganisms to the toxic effects of xenobiotics. In this study, a plant sponge from Luffa cylindrica was used as a carrier for the immobilization of the Stenotrophomonas maltophilia KB2 strain since such a carrier meets the criteria for high-quality carriers, i.e., low price and biodegradability. The optimal immobilization conditions were established as a temperature of 30 °C, pH 7.2, incubation time of 72 h, and an optical density of the culture of 1.4. The strain immobilized in such conditions was used for the biodegradation of naproxen, and an average rate of degradation of 3.8 µg/hour was obtained under cometabolic conditions with glucose. The obtained results indicate that a microbiological preparation based on immobilized cells on a luffa sponge can be used in bioremediation processes where it is necessary to remove the introduced carrier.
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spelling pubmed-95013142022-09-24 Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation Wojcieszyńska, Danuta Klamka, Judyta Marchlewicz, Ariel Potocka, Izabela Żur-Pińska, Joanna Guzik, Urszula Molecules Article Immobilization is a commonly used method in response to the need to increase the resistance of microorganisms to the toxic effects of xenobiotics. In this study, a plant sponge from Luffa cylindrica was used as a carrier for the immobilization of the Stenotrophomonas maltophilia KB2 strain since such a carrier meets the criteria for high-quality carriers, i.e., low price and biodegradability. The optimal immobilization conditions were established as a temperature of 30 °C, pH 7.2, incubation time of 72 h, and an optical density of the culture of 1.4. The strain immobilized in such conditions was used for the biodegradation of naproxen, and an average rate of degradation of 3.8 µg/hour was obtained under cometabolic conditions with glucose. The obtained results indicate that a microbiological preparation based on immobilized cells on a luffa sponge can be used in bioremediation processes where it is necessary to remove the introduced carrier. MDPI 2022-09-07 /pmc/articles/PMC9501314/ /pubmed/36144528 http://dx.doi.org/10.3390/molecules27185795 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wojcieszyńska, Danuta
Klamka, Judyta
Marchlewicz, Ariel
Potocka, Izabela
Żur-Pińska, Joanna
Guzik, Urszula
Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation
title Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation
title_full Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation
title_fullStr Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation
title_full_unstemmed Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation
title_short Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation
title_sort immobilized stenotrophomonas maltophilia kb2 in naproxen degradation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501314/
https://www.ncbi.nlm.nih.gov/pubmed/36144528
http://dx.doi.org/10.3390/molecules27185795
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