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Variability in Skeletal Muscle Protein Synthesis Rates in Critically Ill Patients

(1) Background: Muscle protein synthesis in critically ill patients is, on average, normal despite dramatic muscle loss, but the variation is much larger than in controls. Here, we evaluate if this variation is due to 1) heterogeneity in synthesis rates, 2) morphological variation or infiltrating ce...

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Autores principales: Tjäder, Inga, Klaude, Maria, Hssain, Ali Ait, Guillet, Christelle, Nennesmo, Inger, Wernerman, Jan, Rooyackers, Olav
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501828/
https://www.ncbi.nlm.nih.gov/pubmed/36145109
http://dx.doi.org/10.3390/nu14183733
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author Tjäder, Inga
Klaude, Maria
Hssain, Ali Ait
Guillet, Christelle
Nennesmo, Inger
Wernerman, Jan
Rooyackers, Olav
author_facet Tjäder, Inga
Klaude, Maria
Hssain, Ali Ait
Guillet, Christelle
Nennesmo, Inger
Wernerman, Jan
Rooyackers, Olav
author_sort Tjäder, Inga
collection PubMed
description (1) Background: Muscle protein synthesis in critically ill patients is, on average, normal despite dramatic muscle loss, but the variation is much larger than in controls. Here, we evaluate if this variation is due to 1) heterogeneity in synthesis rates, 2) morphological variation or infiltrating cells, or 3) heterogeneity in the synthesis of different protein fractions. (2) Methods: Muscle biopsies were taken from both legs of critically ill patients (n = 17). Mixed and mitochondrial protein synthesis rates and morphologies were evaluated in both legs. Synthesis rates of myosin and actin were determined in combined biopsies and compared with controls. (3) Results: Muscle protein synthesis rates had a large variability in the patients (1.4–10.8%/day). No differences in mixed and mitochondrial protein synthesis rates between both legs were observed. A microscopic examination revealed no morphological differences between the two legs or any infiltrating inflammatory cells. The synthesis rates for myosin were lower and for actin they were higher in the muscles of critically ill patients, compared with the controls. (4) Conclusions: The large variation in muscle protein synthesis rates in critically ill patients is not the result of heterogeneity in synthesis rates, nor due to infiltrating cells. There are differences in the synthesis rates of different proteins, but these do not explain the larger variations.
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spelling pubmed-95018282022-09-24 Variability in Skeletal Muscle Protein Synthesis Rates in Critically Ill Patients Tjäder, Inga Klaude, Maria Hssain, Ali Ait Guillet, Christelle Nennesmo, Inger Wernerman, Jan Rooyackers, Olav Nutrients Article (1) Background: Muscle protein synthesis in critically ill patients is, on average, normal despite dramatic muscle loss, but the variation is much larger than in controls. Here, we evaluate if this variation is due to 1) heterogeneity in synthesis rates, 2) morphological variation or infiltrating cells, or 3) heterogeneity in the synthesis of different protein fractions. (2) Methods: Muscle biopsies were taken from both legs of critically ill patients (n = 17). Mixed and mitochondrial protein synthesis rates and morphologies were evaluated in both legs. Synthesis rates of myosin and actin were determined in combined biopsies and compared with controls. (3) Results: Muscle protein synthesis rates had a large variability in the patients (1.4–10.8%/day). No differences in mixed and mitochondrial protein synthesis rates between both legs were observed. A microscopic examination revealed no morphological differences between the two legs or any infiltrating inflammatory cells. The synthesis rates for myosin were lower and for actin they were higher in the muscles of critically ill patients, compared with the controls. (4) Conclusions: The large variation in muscle protein synthesis rates in critically ill patients is not the result of heterogeneity in synthesis rates, nor due to infiltrating cells. There are differences in the synthesis rates of different proteins, but these do not explain the larger variations. MDPI 2022-09-10 /pmc/articles/PMC9501828/ /pubmed/36145109 http://dx.doi.org/10.3390/nu14183733 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tjäder, Inga
Klaude, Maria
Hssain, Ali Ait
Guillet, Christelle
Nennesmo, Inger
Wernerman, Jan
Rooyackers, Olav
Variability in Skeletal Muscle Protein Synthesis Rates in Critically Ill Patients
title Variability in Skeletal Muscle Protein Synthesis Rates in Critically Ill Patients
title_full Variability in Skeletal Muscle Protein Synthesis Rates in Critically Ill Patients
title_fullStr Variability in Skeletal Muscle Protein Synthesis Rates in Critically Ill Patients
title_full_unstemmed Variability in Skeletal Muscle Protein Synthesis Rates in Critically Ill Patients
title_short Variability in Skeletal Muscle Protein Synthesis Rates in Critically Ill Patients
title_sort variability in skeletal muscle protein synthesis rates in critically ill patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501828/
https://www.ncbi.nlm.nih.gov/pubmed/36145109
http://dx.doi.org/10.3390/nu14183733
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