Cargando…

Sustainable Extraction, Chemical Profile, Cytotoxic and Antileishmanial Activities In-Vitro of Some Citrus Species Leaves Essential Oils

Anti-leishmanial drugs extracted from natural sources have not been sufficiently explored in the literature. Until now, leishmaniasis treatments have been limited to synthetic and expensive drugs. This study investigated, for the first time, the anti-leishmanial efficacy of essential oils (EOs) from...

Descripción completa

Detalles Bibliográficos
Autores principales: Bouabdallah, Salwa, Cianfaglione, Kevin, Azzouz, Myriam, Batiha, Gaber El-Saber, Alkhuriji, Afrah Fahad, Al-Megrin, Wafa Abdullah I., Ben-Attia, Mossadok, Eldahshan, Omayma A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501829/
https://www.ncbi.nlm.nih.gov/pubmed/36145384
http://dx.doi.org/10.3390/ph15091163
_version_ 1784795563910234112
author Bouabdallah, Salwa
Cianfaglione, Kevin
Azzouz, Myriam
Batiha, Gaber El-Saber
Alkhuriji, Afrah Fahad
Al-Megrin, Wafa Abdullah I.
Ben-Attia, Mossadok
Eldahshan, Omayma A.
author_facet Bouabdallah, Salwa
Cianfaglione, Kevin
Azzouz, Myriam
Batiha, Gaber El-Saber
Alkhuriji, Afrah Fahad
Al-Megrin, Wafa Abdullah I.
Ben-Attia, Mossadok
Eldahshan, Omayma A.
author_sort Bouabdallah, Salwa
collection PubMed
description Anti-leishmanial drugs extracted from natural sources have not been sufficiently explored in the literature. Until now, leishmaniasis treatments have been limited to synthetic and expensive drugs. This study investigated, for the first time, the anti-leishmanial efficacy of essential oils (EOs) from the leaves of Citrus species (C. sinensis, C. limon, and C. clementina). Essential oils were extracted from three species by solvent free microwave extraction (SFME); in addition, lemon oil was also isolated by hydro-distillation (HD). These were investigated using gas chromatography coupled with mass spectrometry (GC–MS) and evaluated against Leishmania species, namely Leishmania major and Leishmania infantum, using a mitochondrial tetrazolium test (MTT) assay. The chemical compositions of Citrus limon EOs obtained by HD and SFME showed some differences. The identified peaks of C. limon (SFME) represented 93.96%, where linalool was the major peak (44.21%), followed by sabinene (14.22%) and ocimene (6.09%). While the hydro-distilled oil of C. limon contained geranial (30.08%), limonene (27.09%), and neral (22.87%) in the identified peaks (96.67%). The identified components of C. clementina leaves oil (68.54%) showed twenty-six compounds, where the predominant compound was geranial (42.40%), followed by neral (26.79%) and limonene (14.48%). However, 89.82% C. sinensis oil was identified, where the major peaks were for neral (27.52%), linalool (25.83%), and geranial (23.44%). HD oil of lemon showed the highest activity against L. major, with moderate toxicity on murine macrophage (RAW 264.7) cells, and possessed the best selectivity index on both Leishmanial species (SI: 3.68; 6.38), followed by C. clementina oil and C. limon using SFME (0.9 ± 0.29, 1.03 ± 0.27, and 1.13 ± 0.3), respectively. C. clementina oil induced the greatest activity on Leishmania infantum, followed by HD lemon and SFME lemon oils (0.32 ± 0.18, 0.52 ± 0.15, and 0.57 ± 0.09, respectively) when compared to Amphotericin B (0.80 ± 0.18 and 0.23 ± 0.13) as a positive control, on both species, respectively. Our study suggests a potent anti-leishmanial activity of lemon oil (HD) on L. major, followed by C. clementina. With the same potency on L. infantum shown by C. clementina oil, followed by HD lemon oil. This effect could be attributed to the major compounds of limonene, citral, and neral, as well as the synergistic effect of other different compounds. These observations could be a starting point for the building of new anti-leishmanial drugs from natural origins, and which combine different EOs containing Citrus cultivars.
format Online
Article
Text
id pubmed-9501829
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95018292022-09-24 Sustainable Extraction, Chemical Profile, Cytotoxic and Antileishmanial Activities In-Vitro of Some Citrus Species Leaves Essential Oils Bouabdallah, Salwa Cianfaglione, Kevin Azzouz, Myriam Batiha, Gaber El-Saber Alkhuriji, Afrah Fahad Al-Megrin, Wafa Abdullah I. Ben-Attia, Mossadok Eldahshan, Omayma A. Pharmaceuticals (Basel) Article Anti-leishmanial drugs extracted from natural sources have not been sufficiently explored in the literature. Until now, leishmaniasis treatments have been limited to synthetic and expensive drugs. This study investigated, for the first time, the anti-leishmanial efficacy of essential oils (EOs) from the leaves of Citrus species (C. sinensis, C. limon, and C. clementina). Essential oils were extracted from three species by solvent free microwave extraction (SFME); in addition, lemon oil was also isolated by hydro-distillation (HD). These were investigated using gas chromatography coupled with mass spectrometry (GC–MS) and evaluated against Leishmania species, namely Leishmania major and Leishmania infantum, using a mitochondrial tetrazolium test (MTT) assay. The chemical compositions of Citrus limon EOs obtained by HD and SFME showed some differences. The identified peaks of C. limon (SFME) represented 93.96%, where linalool was the major peak (44.21%), followed by sabinene (14.22%) and ocimene (6.09%). While the hydro-distilled oil of C. limon contained geranial (30.08%), limonene (27.09%), and neral (22.87%) in the identified peaks (96.67%). The identified components of C. clementina leaves oil (68.54%) showed twenty-six compounds, where the predominant compound was geranial (42.40%), followed by neral (26.79%) and limonene (14.48%). However, 89.82% C. sinensis oil was identified, where the major peaks were for neral (27.52%), linalool (25.83%), and geranial (23.44%). HD oil of lemon showed the highest activity against L. major, with moderate toxicity on murine macrophage (RAW 264.7) cells, and possessed the best selectivity index on both Leishmanial species (SI: 3.68; 6.38), followed by C. clementina oil and C. limon using SFME (0.9 ± 0.29, 1.03 ± 0.27, and 1.13 ± 0.3), respectively. C. clementina oil induced the greatest activity on Leishmania infantum, followed by HD lemon and SFME lemon oils (0.32 ± 0.18, 0.52 ± 0.15, and 0.57 ± 0.09, respectively) when compared to Amphotericin B (0.80 ± 0.18 and 0.23 ± 0.13) as a positive control, on both species, respectively. Our study suggests a potent anti-leishmanial activity of lemon oil (HD) on L. major, followed by C. clementina. With the same potency on L. infantum shown by C. clementina oil, followed by HD lemon oil. This effect could be attributed to the major compounds of limonene, citral, and neral, as well as the synergistic effect of other different compounds. These observations could be a starting point for the building of new anti-leishmanial drugs from natural origins, and which combine different EOs containing Citrus cultivars. MDPI 2022-09-19 /pmc/articles/PMC9501829/ /pubmed/36145384 http://dx.doi.org/10.3390/ph15091163 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bouabdallah, Salwa
Cianfaglione, Kevin
Azzouz, Myriam
Batiha, Gaber El-Saber
Alkhuriji, Afrah Fahad
Al-Megrin, Wafa Abdullah I.
Ben-Attia, Mossadok
Eldahshan, Omayma A.
Sustainable Extraction, Chemical Profile, Cytotoxic and Antileishmanial Activities In-Vitro of Some Citrus Species Leaves Essential Oils
title Sustainable Extraction, Chemical Profile, Cytotoxic and Antileishmanial Activities In-Vitro of Some Citrus Species Leaves Essential Oils
title_full Sustainable Extraction, Chemical Profile, Cytotoxic and Antileishmanial Activities In-Vitro of Some Citrus Species Leaves Essential Oils
title_fullStr Sustainable Extraction, Chemical Profile, Cytotoxic and Antileishmanial Activities In-Vitro of Some Citrus Species Leaves Essential Oils
title_full_unstemmed Sustainable Extraction, Chemical Profile, Cytotoxic and Antileishmanial Activities In-Vitro of Some Citrus Species Leaves Essential Oils
title_short Sustainable Extraction, Chemical Profile, Cytotoxic and Antileishmanial Activities In-Vitro of Some Citrus Species Leaves Essential Oils
title_sort sustainable extraction, chemical profile, cytotoxic and antileishmanial activities in-vitro of some citrus species leaves essential oils
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9501829/
https://www.ncbi.nlm.nih.gov/pubmed/36145384
http://dx.doi.org/10.3390/ph15091163
work_keys_str_mv AT bouabdallahsalwa sustainableextractionchemicalprofilecytotoxicandantileishmanialactivitiesinvitroofsomecitrusspeciesleavesessentialoils
AT cianfaglionekevin sustainableextractionchemicalprofilecytotoxicandantileishmanialactivitiesinvitroofsomecitrusspeciesleavesessentialoils
AT azzouzmyriam sustainableextractionchemicalprofilecytotoxicandantileishmanialactivitiesinvitroofsomecitrusspeciesleavesessentialoils
AT batihagaberelsaber sustainableextractionchemicalprofilecytotoxicandantileishmanialactivitiesinvitroofsomecitrusspeciesleavesessentialoils
AT alkhurijiafrahfahad sustainableextractionchemicalprofilecytotoxicandantileishmanialactivitiesinvitroofsomecitrusspeciesleavesessentialoils
AT almegrinwafaabdullahi sustainableextractionchemicalprofilecytotoxicandantileishmanialactivitiesinvitroofsomecitrusspeciesleavesessentialoils
AT benattiamossadok sustainableextractionchemicalprofilecytotoxicandantileishmanialactivitiesinvitroofsomecitrusspeciesleavesessentialoils
AT eldahshanomaymaa sustainableextractionchemicalprofilecytotoxicandantileishmanialactivitiesinvitroofsomecitrusspeciesleavesessentialoils