Cargando…

Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever

Rickettsia conorii is the causative agent of Mediterranean spotted fever (MSF). Misdiagnosis of MSF may occur with febrile syndromes associated with rash and thrombocytopenia, such as Crimean-Congo hemorrhagic fever (CCHF). This study aimed to determine the prevalence of R. conorii among serum sampl...

Descripción completa

Detalles Bibliográficos
Autores principales: Baseri, Neda, Salehi-Vaziri, Mostafa, Mostafavi, Ehsan, Bagheri Amiri, Fahimeh, Latifian, Mina, Stenos, John, Esmaeili, Saber
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502110/
https://www.ncbi.nlm.nih.gov/pubmed/36145405
http://dx.doi.org/10.3390/pathogens11090973
_version_ 1784795626576281600
author Baseri, Neda
Salehi-Vaziri, Mostafa
Mostafavi, Ehsan
Bagheri Amiri, Fahimeh
Latifian, Mina
Stenos, John
Esmaeili, Saber
author_facet Baseri, Neda
Salehi-Vaziri, Mostafa
Mostafavi, Ehsan
Bagheri Amiri, Fahimeh
Latifian, Mina
Stenos, John
Esmaeili, Saber
author_sort Baseri, Neda
collection PubMed
description Rickettsia conorii is the causative agent of Mediterranean spotted fever (MSF). Misdiagnosis of MSF may occur with febrile syndromes associated with rash and thrombocytopenia, such as Crimean-Congo hemorrhagic fever (CCHF). This study aimed to determine the prevalence of R. conorii among serum samples obtained from 260 suspected CCHF patients with features of MSF in Iran (2018–2020). The quantitative polymerase chain reaction (qPCR) method detected three (1.15%) positive 16S rDNA Rickettsia spp. samples that were classified as R. conorii subsp. conorii, R. conorii subsp. Israelensis, and R. helvetica using the sequencing of gltA, ompA, and 17kDa genes. Furthermore, R. conorii IgM antibodies presented in 38 (14.62%) patients by the enzyme-linked immunosorbent assay (ELISA) method. Out of 97 MSF patients with available paired serum samples, IgM seroconversion and a four-fold increase were observed in 14 (14.43%) and 12 (12.37%) patients, respectively. We concluded that rickettsial agents are present in Iran and may be misdiagnosed with other febrile syndromes.
format Online
Article
Text
id pubmed-9502110
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95021102022-09-24 Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever Baseri, Neda Salehi-Vaziri, Mostafa Mostafavi, Ehsan Bagheri Amiri, Fahimeh Latifian, Mina Stenos, John Esmaeili, Saber Pathogens Article Rickettsia conorii is the causative agent of Mediterranean spotted fever (MSF). Misdiagnosis of MSF may occur with febrile syndromes associated with rash and thrombocytopenia, such as Crimean-Congo hemorrhagic fever (CCHF). This study aimed to determine the prevalence of R. conorii among serum samples obtained from 260 suspected CCHF patients with features of MSF in Iran (2018–2020). The quantitative polymerase chain reaction (qPCR) method detected three (1.15%) positive 16S rDNA Rickettsia spp. samples that were classified as R. conorii subsp. conorii, R. conorii subsp. Israelensis, and R. helvetica using the sequencing of gltA, ompA, and 17kDa genes. Furthermore, R. conorii IgM antibodies presented in 38 (14.62%) patients by the enzyme-linked immunosorbent assay (ELISA) method. Out of 97 MSF patients with available paired serum samples, IgM seroconversion and a four-fold increase were observed in 14 (14.43%) and 12 (12.37%) patients, respectively. We concluded that rickettsial agents are present in Iran and may be misdiagnosed with other febrile syndromes. MDPI 2022-08-26 /pmc/articles/PMC9502110/ /pubmed/36145405 http://dx.doi.org/10.3390/pathogens11090973 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Baseri, Neda
Salehi-Vaziri, Mostafa
Mostafavi, Ehsan
Bagheri Amiri, Fahimeh
Latifian, Mina
Stenos, John
Esmaeili, Saber
Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever
title Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever
title_full Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever
title_fullStr Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever
title_full_unstemmed Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever
title_short Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever
title_sort investigation of rickettsia conorii in patients suspected of having crimean-congo hemorrhagic fever
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502110/
https://www.ncbi.nlm.nih.gov/pubmed/36145405
http://dx.doi.org/10.3390/pathogens11090973
work_keys_str_mv AT baserineda investigationofrickettsiaconoriiinpatientssuspectedofhavingcrimeancongohemorrhagicfever
AT salehivazirimostafa investigationofrickettsiaconoriiinpatientssuspectedofhavingcrimeancongohemorrhagicfever
AT mostafaviehsan investigationofrickettsiaconoriiinpatientssuspectedofhavingcrimeancongohemorrhagicfever
AT bagheriamirifahimeh investigationofrickettsiaconoriiinpatientssuspectedofhavingcrimeancongohemorrhagicfever
AT latifianmina investigationofrickettsiaconoriiinpatientssuspectedofhavingcrimeancongohemorrhagicfever
AT stenosjohn investigationofrickettsiaconoriiinpatientssuspectedofhavingcrimeancongohemorrhagicfever
AT esmaeilisaber investigationofrickettsiaconoriiinpatientssuspectedofhavingcrimeancongohemorrhagicfever