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Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever
Rickettsia conorii is the causative agent of Mediterranean spotted fever (MSF). Misdiagnosis of MSF may occur with febrile syndromes associated with rash and thrombocytopenia, such as Crimean-Congo hemorrhagic fever (CCHF). This study aimed to determine the prevalence of R. conorii among serum sampl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502110/ https://www.ncbi.nlm.nih.gov/pubmed/36145405 http://dx.doi.org/10.3390/pathogens11090973 |
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author | Baseri, Neda Salehi-Vaziri, Mostafa Mostafavi, Ehsan Bagheri Amiri, Fahimeh Latifian, Mina Stenos, John Esmaeili, Saber |
author_facet | Baseri, Neda Salehi-Vaziri, Mostafa Mostafavi, Ehsan Bagheri Amiri, Fahimeh Latifian, Mina Stenos, John Esmaeili, Saber |
author_sort | Baseri, Neda |
collection | PubMed |
description | Rickettsia conorii is the causative agent of Mediterranean spotted fever (MSF). Misdiagnosis of MSF may occur with febrile syndromes associated with rash and thrombocytopenia, such as Crimean-Congo hemorrhagic fever (CCHF). This study aimed to determine the prevalence of R. conorii among serum samples obtained from 260 suspected CCHF patients with features of MSF in Iran (2018–2020). The quantitative polymerase chain reaction (qPCR) method detected three (1.15%) positive 16S rDNA Rickettsia spp. samples that were classified as R. conorii subsp. conorii, R. conorii subsp. Israelensis, and R. helvetica using the sequencing of gltA, ompA, and 17kDa genes. Furthermore, R. conorii IgM antibodies presented in 38 (14.62%) patients by the enzyme-linked immunosorbent assay (ELISA) method. Out of 97 MSF patients with available paired serum samples, IgM seroconversion and a four-fold increase were observed in 14 (14.43%) and 12 (12.37%) patients, respectively. We concluded that rickettsial agents are present in Iran and may be misdiagnosed with other febrile syndromes. |
format | Online Article Text |
id | pubmed-9502110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95021102022-09-24 Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever Baseri, Neda Salehi-Vaziri, Mostafa Mostafavi, Ehsan Bagheri Amiri, Fahimeh Latifian, Mina Stenos, John Esmaeili, Saber Pathogens Article Rickettsia conorii is the causative agent of Mediterranean spotted fever (MSF). Misdiagnosis of MSF may occur with febrile syndromes associated with rash and thrombocytopenia, such as Crimean-Congo hemorrhagic fever (CCHF). This study aimed to determine the prevalence of R. conorii among serum samples obtained from 260 suspected CCHF patients with features of MSF in Iran (2018–2020). The quantitative polymerase chain reaction (qPCR) method detected three (1.15%) positive 16S rDNA Rickettsia spp. samples that were classified as R. conorii subsp. conorii, R. conorii subsp. Israelensis, and R. helvetica using the sequencing of gltA, ompA, and 17kDa genes. Furthermore, R. conorii IgM antibodies presented in 38 (14.62%) patients by the enzyme-linked immunosorbent assay (ELISA) method. Out of 97 MSF patients with available paired serum samples, IgM seroconversion and a four-fold increase were observed in 14 (14.43%) and 12 (12.37%) patients, respectively. We concluded that rickettsial agents are present in Iran and may be misdiagnosed with other febrile syndromes. MDPI 2022-08-26 /pmc/articles/PMC9502110/ /pubmed/36145405 http://dx.doi.org/10.3390/pathogens11090973 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Baseri, Neda Salehi-Vaziri, Mostafa Mostafavi, Ehsan Bagheri Amiri, Fahimeh Latifian, Mina Stenos, John Esmaeili, Saber Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
title | Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
title_full | Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
title_fullStr | Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
title_full_unstemmed | Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
title_short | Investigation of Rickettsia conorii in Patients Suspected of Having Crimean-Congo Hemorrhagic Fever |
title_sort | investigation of rickettsia conorii in patients suspected of having crimean-congo hemorrhagic fever |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502110/ https://www.ncbi.nlm.nih.gov/pubmed/36145405 http://dx.doi.org/10.3390/pathogens11090973 |
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