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GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada

Over the past two decades soybeans grown in western Canada have persistently had lower seed protein than those grown in eastern Canada. To understand the discrepancy in seed protein content between eastern- and western-grown soybeans, RNA-seq and differential expression analysis have been investigat...

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Autores principales: Hooker, Julia C., Nissan, Nour, Luckert, Doris, Zapata, Gerardo, Hou, Anfu, Mohr, Ramona M., Glenn, Aaron J., Barlow, Brent, Daba, Ketema A., Warkentin, Thomas D., Lefebvre, François, Golshani, Ashkan, Cober, Elroy R., Samanfar, Bahram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502396/
https://www.ncbi.nlm.nih.gov/pubmed/36145738
http://dx.doi.org/10.3390/plants11182337
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author Hooker, Julia C.
Nissan, Nour
Luckert, Doris
Zapata, Gerardo
Hou, Anfu
Mohr, Ramona M.
Glenn, Aaron J.
Barlow, Brent
Daba, Ketema A.
Warkentin, Thomas D.
Lefebvre, François
Golshani, Ashkan
Cober, Elroy R.
Samanfar, Bahram
author_facet Hooker, Julia C.
Nissan, Nour
Luckert, Doris
Zapata, Gerardo
Hou, Anfu
Mohr, Ramona M.
Glenn, Aaron J.
Barlow, Brent
Daba, Ketema A.
Warkentin, Thomas D.
Lefebvre, François
Golshani, Ashkan
Cober, Elroy R.
Samanfar, Bahram
author_sort Hooker, Julia C.
collection PubMed
description Over the past two decades soybeans grown in western Canada have persistently had lower seed protein than those grown in eastern Canada. To understand the discrepancy in seed protein content between eastern- and western-grown soybeans, RNA-seq and differential expression analysis have been investigated. Ten soybean genotypes, ranging from low to high in seed protein content, were grown in four locations across eastern (Ottawa) and western (Morden, Brandon, and Saskatoon) Canada. Differential expression analysis revealed 34 differentially expressed genes encoding Glycine max Sugars Will Eventually be Exported Transporters (GmSWEETs), including paralogs GmSWEET29 and GmSWEET34 (AtSWEET2 homologs) that were consistently upregulated across all ten genotypes in each of the western locations over three years. GmSWEET29 and GmSWEET34 are likely candidates underlying the lower seed protein content of western soybeans. GmSWEET20 (AtSWEET12 homolog) was downregulated in the western locations and may also play a role in lower seed protein content. These findings are valuable for improving soybean agriculture in western growing regions, establishing more strategic and efficient agricultural practices.
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spelling pubmed-95023962022-09-24 GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada Hooker, Julia C. Nissan, Nour Luckert, Doris Zapata, Gerardo Hou, Anfu Mohr, Ramona M. Glenn, Aaron J. Barlow, Brent Daba, Ketema A. Warkentin, Thomas D. Lefebvre, François Golshani, Ashkan Cober, Elroy R. Samanfar, Bahram Plants (Basel) Article Over the past two decades soybeans grown in western Canada have persistently had lower seed protein than those grown in eastern Canada. To understand the discrepancy in seed protein content between eastern- and western-grown soybeans, RNA-seq and differential expression analysis have been investigated. Ten soybean genotypes, ranging from low to high in seed protein content, were grown in four locations across eastern (Ottawa) and western (Morden, Brandon, and Saskatoon) Canada. Differential expression analysis revealed 34 differentially expressed genes encoding Glycine max Sugars Will Eventually be Exported Transporters (GmSWEETs), including paralogs GmSWEET29 and GmSWEET34 (AtSWEET2 homologs) that were consistently upregulated across all ten genotypes in each of the western locations over three years. GmSWEET29 and GmSWEET34 are likely candidates underlying the lower seed protein content of western soybeans. GmSWEET20 (AtSWEET12 homolog) was downregulated in the western locations and may also play a role in lower seed protein content. These findings are valuable for improving soybean agriculture in western growing regions, establishing more strategic and efficient agricultural practices. MDPI 2022-09-07 /pmc/articles/PMC9502396/ /pubmed/36145738 http://dx.doi.org/10.3390/plants11182337 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hooker, Julia C.
Nissan, Nour
Luckert, Doris
Zapata, Gerardo
Hou, Anfu
Mohr, Ramona M.
Glenn, Aaron J.
Barlow, Brent
Daba, Ketema A.
Warkentin, Thomas D.
Lefebvre, François
Golshani, Ashkan
Cober, Elroy R.
Samanfar, Bahram
GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada
title GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada
title_full GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada
title_fullStr GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada
title_full_unstemmed GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada
title_short GmSWEET29 and Paralog GmSWEET34 Are Differentially Expressed between Soybeans Grown in Eastern and Western Canada
title_sort gmsweet29 and paralog gmsweet34 are differentially expressed between soybeans grown in eastern and western canada
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502396/
https://www.ncbi.nlm.nih.gov/pubmed/36145738
http://dx.doi.org/10.3390/plants11182337
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