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Changes in Serum IgG Glycosylation Patterns for Abdominal Aortic Aneurysm Patients

Background: B cells and autoantibodies play an important role in the pathogenesis of abdominal aortic aneurysm (AAA). IgG glycosylations are highly valued as potential disease biomarkers and therapeutic targets. Methods: Lectin microarray was applied to analyze the expression profile of serum IgG gl...

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Autores principales: Li, Siting, Meng, Jingjing, Lv, Yanze, Wang, Qian, Tian, Xinping, Li, Mengtao, Zeng, Xiaofeng, Hu, Chaojun, Zheng, Yuehong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502462/
https://www.ncbi.nlm.nih.gov/pubmed/36135436
http://dx.doi.org/10.3390/jcdd9090291
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author Li, Siting
Meng, Jingjing
Lv, Yanze
Wang, Qian
Tian, Xinping
Li, Mengtao
Zeng, Xiaofeng
Hu, Chaojun
Zheng, Yuehong
author_facet Li, Siting
Meng, Jingjing
Lv, Yanze
Wang, Qian
Tian, Xinping
Li, Mengtao
Zeng, Xiaofeng
Hu, Chaojun
Zheng, Yuehong
author_sort Li, Siting
collection PubMed
description Background: B cells and autoantibodies play an important role in the pathogenesis of abdominal aortic aneurysm (AAA). IgG glycosylations are highly valued as potential disease biomarkers and therapeutic targets. Methods: Lectin microarray was applied to analyze the expression profile of serum IgG glycosylation in 75 patients with AAA, 68 autoimmune disease controls, and 100 healthy controls. Lectin blots were performed to validate the differences. The clinical relevance of lectins binding from the microarray results was explored in AAA patients. Results: Significantly lower binding level of SBA (preferred GalNAc) was observed for the AAA group compared with DCs (p < 0.001) and HCs (p = 0.049). A significantly lower binding level of ConA (preferred mannose) was observed in patients with aneurysm diameter >5 cm. Significantly higher binding of CSA (preferred GalNAc) was present for dyslipidemia patients, whereas a lower binding level of AAL (preferred fucose) was observed for hypertensive patients. Patients with diabetes had lower binding levels of IRA (preferred GalNAc) and HPA (preferred GalNAc) compared with those not with DM. PTL-L (R = 0.36, p = 0.0015, preferred GalNAc) was positively associated with aneurysm diameters, whereas DSL (R = 0.28, p = 0.014, preferred (GlcNAc)2-4) was positively associated with patients’ age. Symptomatic patients had a lower binding level of ConA (p = 0.032), and patients with coronary heart disease had higher binding levels of STL (p = 0.0029, preferred GlcNAc). Patients with ILT bound less with black bean crude (p = 0.04, preferred GalNAc). Conclusions: AAA was associated with a decreased IgG binding level of SBA (recognizing glycan GalNAc). Symptomatic patients with aneurysm <5 cm had a higher binding level of ConA (preferred mannose). Coronary heart disease and elder age were associated with increased IgG bisecting GlcNAc. IgG O-glycosylation (GalNAc) may play an important role in AAA pathogenesis and progression.
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spelling pubmed-95024622022-09-24 Changes in Serum IgG Glycosylation Patterns for Abdominal Aortic Aneurysm Patients Li, Siting Meng, Jingjing Lv, Yanze Wang, Qian Tian, Xinping Li, Mengtao Zeng, Xiaofeng Hu, Chaojun Zheng, Yuehong J Cardiovasc Dev Dis Article Background: B cells and autoantibodies play an important role in the pathogenesis of abdominal aortic aneurysm (AAA). IgG glycosylations are highly valued as potential disease biomarkers and therapeutic targets. Methods: Lectin microarray was applied to analyze the expression profile of serum IgG glycosylation in 75 patients with AAA, 68 autoimmune disease controls, and 100 healthy controls. Lectin blots were performed to validate the differences. The clinical relevance of lectins binding from the microarray results was explored in AAA patients. Results: Significantly lower binding level of SBA (preferred GalNAc) was observed for the AAA group compared with DCs (p < 0.001) and HCs (p = 0.049). A significantly lower binding level of ConA (preferred mannose) was observed in patients with aneurysm diameter >5 cm. Significantly higher binding of CSA (preferred GalNAc) was present for dyslipidemia patients, whereas a lower binding level of AAL (preferred fucose) was observed for hypertensive patients. Patients with diabetes had lower binding levels of IRA (preferred GalNAc) and HPA (preferred GalNAc) compared with those not with DM. PTL-L (R = 0.36, p = 0.0015, preferred GalNAc) was positively associated with aneurysm diameters, whereas DSL (R = 0.28, p = 0.014, preferred (GlcNAc)2-4) was positively associated with patients’ age. Symptomatic patients had a lower binding level of ConA (p = 0.032), and patients with coronary heart disease had higher binding levels of STL (p = 0.0029, preferred GlcNAc). Patients with ILT bound less with black bean crude (p = 0.04, preferred GalNAc). Conclusions: AAA was associated with a decreased IgG binding level of SBA (recognizing glycan GalNAc). Symptomatic patients with aneurysm <5 cm had a higher binding level of ConA (preferred mannose). Coronary heart disease and elder age were associated with increased IgG bisecting GlcNAc. IgG O-glycosylation (GalNAc) may play an important role in AAA pathogenesis and progression. MDPI 2022-09-01 /pmc/articles/PMC9502462/ /pubmed/36135436 http://dx.doi.org/10.3390/jcdd9090291 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Siting
Meng, Jingjing
Lv, Yanze
Wang, Qian
Tian, Xinping
Li, Mengtao
Zeng, Xiaofeng
Hu, Chaojun
Zheng, Yuehong
Changes in Serum IgG Glycosylation Patterns for Abdominal Aortic Aneurysm Patients
title Changes in Serum IgG Glycosylation Patterns for Abdominal Aortic Aneurysm Patients
title_full Changes in Serum IgG Glycosylation Patterns for Abdominal Aortic Aneurysm Patients
title_fullStr Changes in Serum IgG Glycosylation Patterns for Abdominal Aortic Aneurysm Patients
title_full_unstemmed Changes in Serum IgG Glycosylation Patterns for Abdominal Aortic Aneurysm Patients
title_short Changes in Serum IgG Glycosylation Patterns for Abdominal Aortic Aneurysm Patients
title_sort changes in serum igg glycosylation patterns for abdominal aortic aneurysm patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502462/
https://www.ncbi.nlm.nih.gov/pubmed/36135436
http://dx.doi.org/10.3390/jcdd9090291
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