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Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53
Wound infections are prone to attacks from infectious pathogens, including multidrug resistant bacteria that render conventional antimicrobials ineffective. Recently, lysins have been proposed as alternatives to conventional antimicrobials to tackle the menace of multidrug resistance pathogens. The...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502541/ https://www.ncbi.nlm.nih.gov/pubmed/36146767 http://dx.doi.org/10.3390/v14091956 |
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author | Li, Changchang Nyaruaba, Raphael Zhao, Xiaowei Xue, Heng Li, Yuhong Yang, Hang Wei, Hongping |
author_facet | Li, Changchang Nyaruaba, Raphael Zhao, Xiaowei Xue, Heng Li, Yuhong Yang, Hang Wei, Hongping |
author_sort | Li, Changchang |
collection | PubMed |
description | Wound infections are prone to attacks from infectious pathogens, including multidrug resistant bacteria that render conventional antimicrobials ineffective. Recently, lysins have been proposed as alternatives to conventional antimicrobials to tackle the menace of multidrug resistance pathogens. The coupling of lysins with a material that will cover the wound may prove beneficial in both protecting and treating wound infections. Hence, in this study, a Gram-negative lysin, LysP53, was coupled with a thermosensitive hydrogel, poloxamer P407, and its efficacy to treat wound infection was tested. In vitro, the addition of LysP53 to the poloxamer did not affect its thermosensitive characteristics, nor did it affect the hydrogel structure. Moreover, the lysin hydrogel could hydrolyze the peptidoglycan, demonstrating that it may have bactericidal activity. Up to 10.4% of LysP53 was released from the hydrogel gradually within 24 h, which led to a 4-log reduction of stationary phase Acinetobacter baumannii. Lastly, the lysin hydrogel was found safe with no cytotoxic effects observed in cells. Ex vivo, LysP53 hydrogel could inhibit bacterial growth on a pig skin decolonization model, with 3-log differences compared to non-treated groups. Overall, our results suggest that lysin-loaded hydrogels may provide a novel solution to treat wound infections caused by resistant bacteria. |
format | Online Article Text |
id | pubmed-9502541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95025412022-09-24 Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53 Li, Changchang Nyaruaba, Raphael Zhao, Xiaowei Xue, Heng Li, Yuhong Yang, Hang Wei, Hongping Viruses Article Wound infections are prone to attacks from infectious pathogens, including multidrug resistant bacteria that render conventional antimicrobials ineffective. Recently, lysins have been proposed as alternatives to conventional antimicrobials to tackle the menace of multidrug resistance pathogens. The coupling of lysins with a material that will cover the wound may prove beneficial in both protecting and treating wound infections. Hence, in this study, a Gram-negative lysin, LysP53, was coupled with a thermosensitive hydrogel, poloxamer P407, and its efficacy to treat wound infection was tested. In vitro, the addition of LysP53 to the poloxamer did not affect its thermosensitive characteristics, nor did it affect the hydrogel structure. Moreover, the lysin hydrogel could hydrolyze the peptidoglycan, demonstrating that it may have bactericidal activity. Up to 10.4% of LysP53 was released from the hydrogel gradually within 24 h, which led to a 4-log reduction of stationary phase Acinetobacter baumannii. Lastly, the lysin hydrogel was found safe with no cytotoxic effects observed in cells. Ex vivo, LysP53 hydrogel could inhibit bacterial growth on a pig skin decolonization model, with 3-log differences compared to non-treated groups. Overall, our results suggest that lysin-loaded hydrogels may provide a novel solution to treat wound infections caused by resistant bacteria. MDPI 2022-09-03 /pmc/articles/PMC9502541/ /pubmed/36146767 http://dx.doi.org/10.3390/v14091956 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Changchang Nyaruaba, Raphael Zhao, Xiaowei Xue, Heng Li, Yuhong Yang, Hang Wei, Hongping Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53 |
title | Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53 |
title_full | Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53 |
title_fullStr | Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53 |
title_full_unstemmed | Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53 |
title_short | Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53 |
title_sort | thermosensitive hydrogel wound dressing loaded with bacteriophage lysin lysp53 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502541/ https://www.ncbi.nlm.nih.gov/pubmed/36146767 http://dx.doi.org/10.3390/v14091956 |
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