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Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53

Wound infections are prone to attacks from infectious pathogens, including multidrug resistant bacteria that render conventional antimicrobials ineffective. Recently, lysins have been proposed as alternatives to conventional antimicrobials to tackle the menace of multidrug resistance pathogens. The...

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Autores principales: Li, Changchang, Nyaruaba, Raphael, Zhao, Xiaowei, Xue, Heng, Li, Yuhong, Yang, Hang, Wei, Hongping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502541/
https://www.ncbi.nlm.nih.gov/pubmed/36146767
http://dx.doi.org/10.3390/v14091956
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author Li, Changchang
Nyaruaba, Raphael
Zhao, Xiaowei
Xue, Heng
Li, Yuhong
Yang, Hang
Wei, Hongping
author_facet Li, Changchang
Nyaruaba, Raphael
Zhao, Xiaowei
Xue, Heng
Li, Yuhong
Yang, Hang
Wei, Hongping
author_sort Li, Changchang
collection PubMed
description Wound infections are prone to attacks from infectious pathogens, including multidrug resistant bacteria that render conventional antimicrobials ineffective. Recently, lysins have been proposed as alternatives to conventional antimicrobials to tackle the menace of multidrug resistance pathogens. The coupling of lysins with a material that will cover the wound may prove beneficial in both protecting and treating wound infections. Hence, in this study, a Gram-negative lysin, LysP53, was coupled with a thermosensitive hydrogel, poloxamer P407, and its efficacy to treat wound infection was tested. In vitro, the addition of LysP53 to the poloxamer did not affect its thermosensitive characteristics, nor did it affect the hydrogel structure. Moreover, the lysin hydrogel could hydrolyze the peptidoglycan, demonstrating that it may have bactericidal activity. Up to 10.4% of LysP53 was released from the hydrogel gradually within 24 h, which led to a 4-log reduction of stationary phase Acinetobacter baumannii. Lastly, the lysin hydrogel was found safe with no cytotoxic effects observed in cells. Ex vivo, LysP53 hydrogel could inhibit bacterial growth on a pig skin decolonization model, with 3-log differences compared to non-treated groups. Overall, our results suggest that lysin-loaded hydrogels may provide a novel solution to treat wound infections caused by resistant bacteria.
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spelling pubmed-95025412022-09-24 Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53 Li, Changchang Nyaruaba, Raphael Zhao, Xiaowei Xue, Heng Li, Yuhong Yang, Hang Wei, Hongping Viruses Article Wound infections are prone to attacks from infectious pathogens, including multidrug resistant bacteria that render conventional antimicrobials ineffective. Recently, lysins have been proposed as alternatives to conventional antimicrobials to tackle the menace of multidrug resistance pathogens. The coupling of lysins with a material that will cover the wound may prove beneficial in both protecting and treating wound infections. Hence, in this study, a Gram-negative lysin, LysP53, was coupled with a thermosensitive hydrogel, poloxamer P407, and its efficacy to treat wound infection was tested. In vitro, the addition of LysP53 to the poloxamer did not affect its thermosensitive characteristics, nor did it affect the hydrogel structure. Moreover, the lysin hydrogel could hydrolyze the peptidoglycan, demonstrating that it may have bactericidal activity. Up to 10.4% of LysP53 was released from the hydrogel gradually within 24 h, which led to a 4-log reduction of stationary phase Acinetobacter baumannii. Lastly, the lysin hydrogel was found safe with no cytotoxic effects observed in cells. Ex vivo, LysP53 hydrogel could inhibit bacterial growth on a pig skin decolonization model, with 3-log differences compared to non-treated groups. Overall, our results suggest that lysin-loaded hydrogels may provide a novel solution to treat wound infections caused by resistant bacteria. MDPI 2022-09-03 /pmc/articles/PMC9502541/ /pubmed/36146767 http://dx.doi.org/10.3390/v14091956 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Changchang
Nyaruaba, Raphael
Zhao, Xiaowei
Xue, Heng
Li, Yuhong
Yang, Hang
Wei, Hongping
Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53
title Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53
title_full Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53
title_fullStr Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53
title_full_unstemmed Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53
title_short Thermosensitive Hydrogel Wound Dressing Loaded with Bacteriophage Lysin LysP53
title_sort thermosensitive hydrogel wound dressing loaded with bacteriophage lysin lysp53
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502541/
https://www.ncbi.nlm.nih.gov/pubmed/36146767
http://dx.doi.org/10.3390/v14091956
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