Cargando…
Inhibition of Cell-Free Translation and Replication of Tobacco Mosaic Virus RNA by Exogenously Added 5′-Proximal Fragments of the Genomic RNA
Replication proteins of tobacco mosaic virus (TMV), a positive-sense RNA virus, co-translationally bind to a 5′-proximal ~70-nucleotide (nt) region of the genomic RNA, referred to as the nuclease-resistant (NR) region for replication template selection. Therefore, disruption of the interaction betwe...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502800/ https://www.ncbi.nlm.nih.gov/pubmed/36146772 http://dx.doi.org/10.3390/v14091962 |
_version_ | 1784795795010093056 |
---|---|
author | Yoshida, Tetsuya Ishikawa, Masayuki Ishibashi, Kazuhiro |
author_facet | Yoshida, Tetsuya Ishikawa, Masayuki Ishibashi, Kazuhiro |
author_sort | Yoshida, Tetsuya |
collection | PubMed |
description | Replication proteins of tobacco mosaic virus (TMV), a positive-sense RNA virus, co-translationally bind to a 5′-proximal ~70-nucleotide (nt) region of the genomic RNA, referred to as the nuclease-resistant (NR) region for replication template selection. Therefore, disruption of the interaction between the viral replication proteins and viral genomic RNA is expected to inhibit the replication of TMV. In this study, we demonstrate that the addition of small RNA fragments (18–33 nts in length) derived from different regions within the NR region inhibit the binding of TMV replication proteins to viral RNA and TMV RNA replication in a cell-free system. Intriguingly, some of the small RNA fragments also inhibited the translation of mRNA in a sequence-nonspecific manner. These results highlight the pleiotropic roles of the 5′-proximal region of the TMV genome. |
format | Online Article Text |
id | pubmed-9502800 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95028002022-09-24 Inhibition of Cell-Free Translation and Replication of Tobacco Mosaic Virus RNA by Exogenously Added 5′-Proximal Fragments of the Genomic RNA Yoshida, Tetsuya Ishikawa, Masayuki Ishibashi, Kazuhiro Viruses Communication Replication proteins of tobacco mosaic virus (TMV), a positive-sense RNA virus, co-translationally bind to a 5′-proximal ~70-nucleotide (nt) region of the genomic RNA, referred to as the nuclease-resistant (NR) region for replication template selection. Therefore, disruption of the interaction between the viral replication proteins and viral genomic RNA is expected to inhibit the replication of TMV. In this study, we demonstrate that the addition of small RNA fragments (18–33 nts in length) derived from different regions within the NR region inhibit the binding of TMV replication proteins to viral RNA and TMV RNA replication in a cell-free system. Intriguingly, some of the small RNA fragments also inhibited the translation of mRNA in a sequence-nonspecific manner. These results highlight the pleiotropic roles of the 5′-proximal region of the TMV genome. MDPI 2022-09-04 /pmc/articles/PMC9502800/ /pubmed/36146772 http://dx.doi.org/10.3390/v14091962 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Yoshida, Tetsuya Ishikawa, Masayuki Ishibashi, Kazuhiro Inhibition of Cell-Free Translation and Replication of Tobacco Mosaic Virus RNA by Exogenously Added 5′-Proximal Fragments of the Genomic RNA |
title | Inhibition of Cell-Free Translation and Replication of Tobacco Mosaic Virus RNA by Exogenously Added 5′-Proximal Fragments of the Genomic RNA |
title_full | Inhibition of Cell-Free Translation and Replication of Tobacco Mosaic Virus RNA by Exogenously Added 5′-Proximal Fragments of the Genomic RNA |
title_fullStr | Inhibition of Cell-Free Translation and Replication of Tobacco Mosaic Virus RNA by Exogenously Added 5′-Proximal Fragments of the Genomic RNA |
title_full_unstemmed | Inhibition of Cell-Free Translation and Replication of Tobacco Mosaic Virus RNA by Exogenously Added 5′-Proximal Fragments of the Genomic RNA |
title_short | Inhibition of Cell-Free Translation and Replication of Tobacco Mosaic Virus RNA by Exogenously Added 5′-Proximal Fragments of the Genomic RNA |
title_sort | inhibition of cell-free translation and replication of tobacco mosaic virus rna by exogenously added 5′-proximal fragments of the genomic rna |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502800/ https://www.ncbi.nlm.nih.gov/pubmed/36146772 http://dx.doi.org/10.3390/v14091962 |
work_keys_str_mv | AT yoshidatetsuya inhibitionofcellfreetranslationandreplicationoftobaccomosaicvirusrnabyexogenouslyadded5proximalfragmentsofthegenomicrna AT ishikawamasayuki inhibitionofcellfreetranslationandreplicationoftobaccomosaicvirusrnabyexogenouslyadded5proximalfragmentsofthegenomicrna AT ishibashikazuhiro inhibitionofcellfreetranslationandreplicationoftobaccomosaicvirusrnabyexogenouslyadded5proximalfragmentsofthegenomicrna |