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Overexpression of PagERF072 from Poplar Improves Salt Tolerance

Extreme environments, especially drought and high salt conditions, seriously affect plant growth and development. Ethylene-responsive factor (ERF) transcription factors play an important role in salt stress response. In this study, a significantly upregulated ERF gene was identified in 84K (Populus...

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Autores principales: Zhang, Xuemei, Cheng, Zihan, Yao, Wenjing, Gao, Yuan, Fan, Gaofeng, Guo, Qing, Zhou, Boru, Jiang, Tingbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502824/
https://www.ncbi.nlm.nih.gov/pubmed/36142609
http://dx.doi.org/10.3390/ijms231810707
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author Zhang, Xuemei
Cheng, Zihan
Yao, Wenjing
Gao, Yuan
Fan, Gaofeng
Guo, Qing
Zhou, Boru
Jiang, Tingbo
author_facet Zhang, Xuemei
Cheng, Zihan
Yao, Wenjing
Gao, Yuan
Fan, Gaofeng
Guo, Qing
Zhou, Boru
Jiang, Tingbo
author_sort Zhang, Xuemei
collection PubMed
description Extreme environments, especially drought and high salt conditions, seriously affect plant growth and development. Ethylene-responsive factor (ERF) transcription factors play an important role in salt stress response. In this study, a significantly upregulated ERF gene was identified in 84K (Populus alba × P. glandulosa), which was named PagERF072. PagERF072 was confirmed to be a nuclear-localized protein. The results of yeast two-hybrid (Y2H) assay showed that PagERF072 protein exhibited no self-activating activity, and yeast one-hybrid (Y1H) demonstrated that PagERF072 could specifically bind to GCC-box element. Under salt stress, the transgenic poplar lines overexpressing PagERF072 showed improved salt tolerance. The activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) in transgenic poplars were significantly increased relative to those of wild-type (WT) plants, whereas malondialdehyde (MDA) content showed an opposite trend. In addition, reactive oxygen species (ROS) was significantly reduced, and the expression levels of POD- and SOD-related genes were significantly increased in transgenic poplars under salt stress compared with WT. All results indicate that overexpression of the PagERF072 gene can improve the salt tolerance of transgenic poplars.
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spelling pubmed-95028242022-09-24 Overexpression of PagERF072 from Poplar Improves Salt Tolerance Zhang, Xuemei Cheng, Zihan Yao, Wenjing Gao, Yuan Fan, Gaofeng Guo, Qing Zhou, Boru Jiang, Tingbo Int J Mol Sci Article Extreme environments, especially drought and high salt conditions, seriously affect plant growth and development. Ethylene-responsive factor (ERF) transcription factors play an important role in salt stress response. In this study, a significantly upregulated ERF gene was identified in 84K (Populus alba × P. glandulosa), which was named PagERF072. PagERF072 was confirmed to be a nuclear-localized protein. The results of yeast two-hybrid (Y2H) assay showed that PagERF072 protein exhibited no self-activating activity, and yeast one-hybrid (Y1H) demonstrated that PagERF072 could specifically bind to GCC-box element. Under salt stress, the transgenic poplar lines overexpressing PagERF072 showed improved salt tolerance. The activities of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) in transgenic poplars were significantly increased relative to those of wild-type (WT) plants, whereas malondialdehyde (MDA) content showed an opposite trend. In addition, reactive oxygen species (ROS) was significantly reduced, and the expression levels of POD- and SOD-related genes were significantly increased in transgenic poplars under salt stress compared with WT. All results indicate that overexpression of the PagERF072 gene can improve the salt tolerance of transgenic poplars. MDPI 2022-09-14 /pmc/articles/PMC9502824/ /pubmed/36142609 http://dx.doi.org/10.3390/ijms231810707 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Xuemei
Cheng, Zihan
Yao, Wenjing
Gao, Yuan
Fan, Gaofeng
Guo, Qing
Zhou, Boru
Jiang, Tingbo
Overexpression of PagERF072 from Poplar Improves Salt Tolerance
title Overexpression of PagERF072 from Poplar Improves Salt Tolerance
title_full Overexpression of PagERF072 from Poplar Improves Salt Tolerance
title_fullStr Overexpression of PagERF072 from Poplar Improves Salt Tolerance
title_full_unstemmed Overexpression of PagERF072 from Poplar Improves Salt Tolerance
title_short Overexpression of PagERF072 from Poplar Improves Salt Tolerance
title_sort overexpression of pagerf072 from poplar improves salt tolerance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9502824/
https://www.ncbi.nlm.nih.gov/pubmed/36142609
http://dx.doi.org/10.3390/ijms231810707
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