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Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus
The nematocidal activity of an Oxalis tetraphylla hydroalcoholic extract against the nematode Haemonchus contortus (Hc) was assessed in vitro and the major compounds associated with nematocidal activity were identified. One hydroalcoholic extract was obtained from O. tetraphylla stems and leaves (Ot...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503173/ https://www.ncbi.nlm.nih.gov/pubmed/36145456 http://dx.doi.org/10.3390/pathogens11091024 |
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author | Ocampo-Gutiérrez, Ana Yuridia Hernández-Velázquez, Víctor Manuel Zamilpa, Alejandro López-Arellano, María Eugenia Olmedo-Juárez, Agustín Higuera-Piedrahita, Rosa Isabel Delgado-Núñez, Edgar Jesús González-Cortázar, Manasés Mendoza-de Gives, Pedro |
author_facet | Ocampo-Gutiérrez, Ana Yuridia Hernández-Velázquez, Víctor Manuel Zamilpa, Alejandro López-Arellano, María Eugenia Olmedo-Juárez, Agustín Higuera-Piedrahita, Rosa Isabel Delgado-Núñez, Edgar Jesús González-Cortázar, Manasés Mendoza-de Gives, Pedro |
author_sort | Ocampo-Gutiérrez, Ana Yuridia |
collection | PubMed |
description | The nematocidal activity of an Oxalis tetraphylla hydroalcoholic extract against the nematode Haemonchus contortus (Hc) was assessed in vitro and the major compounds associated with nematocidal activity were identified. One hydroalcoholic extract was obtained from O. tetraphylla stems and leaves (Ot HE-SLE). The in vitro lethal concentrations (LC50 and LC90) against both eggs and exsheathed Hc infective larvae (L3) were assessed. Ot HE-SLE showed a potent ovicidal activity (LC50 = 0.213 mg/mL; LC90 = 0.71 mg/mL) and larvicidal effect (LC50 = 28.01 mg/mL; LC90 = 69.3 mg/mL). Later on, the extract was bipartitioned to obtain an ethyl acetate phase (EtOAc-Ph) and an aqueous phase (Aq-Ph). Both phases were assessed against Hc eggs at 0.25 and 1.0 mg/mL concentrations. The results with EtOAc-Ph showed 93.6% ovicidal activity, while 96.6% was recorded with Aq-Ph at 48 h post-confrontation (PC). In the case of larvicidal activity, both phases were assessed at 28 mg/mL; Aq-Ph showed >80% larvicidal activity 24 and 72 h PC, while EtOAc-Ph did not show important activity. HPLC analysis showed the presence of coumaric acid and flavonols. Flavonol compounds were the major compounds and were associated with the nematocidal activity. Additionally, the Aq-Ph that showed the highest activity was purified, and the fraction F3 showed the highest nematocidal activity. |
format | Online Article Text |
id | pubmed-9503173 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95031732022-09-24 Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus Ocampo-Gutiérrez, Ana Yuridia Hernández-Velázquez, Víctor Manuel Zamilpa, Alejandro López-Arellano, María Eugenia Olmedo-Juárez, Agustín Higuera-Piedrahita, Rosa Isabel Delgado-Núñez, Edgar Jesús González-Cortázar, Manasés Mendoza-de Gives, Pedro Pathogens Article The nematocidal activity of an Oxalis tetraphylla hydroalcoholic extract against the nematode Haemonchus contortus (Hc) was assessed in vitro and the major compounds associated with nematocidal activity were identified. One hydroalcoholic extract was obtained from O. tetraphylla stems and leaves (Ot HE-SLE). The in vitro lethal concentrations (LC50 and LC90) against both eggs and exsheathed Hc infective larvae (L3) were assessed. Ot HE-SLE showed a potent ovicidal activity (LC50 = 0.213 mg/mL; LC90 = 0.71 mg/mL) and larvicidal effect (LC50 = 28.01 mg/mL; LC90 = 69.3 mg/mL). Later on, the extract was bipartitioned to obtain an ethyl acetate phase (EtOAc-Ph) and an aqueous phase (Aq-Ph). Both phases were assessed against Hc eggs at 0.25 and 1.0 mg/mL concentrations. The results with EtOAc-Ph showed 93.6% ovicidal activity, while 96.6% was recorded with Aq-Ph at 48 h post-confrontation (PC). In the case of larvicidal activity, both phases were assessed at 28 mg/mL; Aq-Ph showed >80% larvicidal activity 24 and 72 h PC, while EtOAc-Ph did not show important activity. HPLC analysis showed the presence of coumaric acid and flavonols. Flavonol compounds were the major compounds and were associated with the nematocidal activity. Additionally, the Aq-Ph that showed the highest activity was purified, and the fraction F3 showed the highest nematocidal activity. MDPI 2022-09-08 /pmc/articles/PMC9503173/ /pubmed/36145456 http://dx.doi.org/10.3390/pathogens11091024 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ocampo-Gutiérrez, Ana Yuridia Hernández-Velázquez, Víctor Manuel Zamilpa, Alejandro López-Arellano, María Eugenia Olmedo-Juárez, Agustín Higuera-Piedrahita, Rosa Isabel Delgado-Núñez, Edgar Jesús González-Cortázar, Manasés Mendoza-de Gives, Pedro Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus |
title | Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus |
title_full | Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus |
title_fullStr | Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus |
title_full_unstemmed | Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus |
title_short | Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus |
title_sort | oxalis tetraphylla (class: magnoliopsidae) possess flavonoid phytoconstituents with nematocidal activity against haemonchus contortus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503173/ https://www.ncbi.nlm.nih.gov/pubmed/36145456 http://dx.doi.org/10.3390/pathogens11091024 |
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