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Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus

The nematocidal activity of an Oxalis tetraphylla hydroalcoholic extract against the nematode Haemonchus contortus (Hc) was assessed in vitro and the major compounds associated with nematocidal activity were identified. One hydroalcoholic extract was obtained from O. tetraphylla stems and leaves (Ot...

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Autores principales: Ocampo-Gutiérrez, Ana Yuridia, Hernández-Velázquez, Víctor Manuel, Zamilpa, Alejandro, López-Arellano, María Eugenia, Olmedo-Juárez, Agustín, Higuera-Piedrahita, Rosa Isabel, Delgado-Núñez, Edgar Jesús, González-Cortázar, Manasés, Mendoza-de Gives, Pedro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503173/
https://www.ncbi.nlm.nih.gov/pubmed/36145456
http://dx.doi.org/10.3390/pathogens11091024
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author Ocampo-Gutiérrez, Ana Yuridia
Hernández-Velázquez, Víctor Manuel
Zamilpa, Alejandro
López-Arellano, María Eugenia
Olmedo-Juárez, Agustín
Higuera-Piedrahita, Rosa Isabel
Delgado-Núñez, Edgar Jesús
González-Cortázar, Manasés
Mendoza-de Gives, Pedro
author_facet Ocampo-Gutiérrez, Ana Yuridia
Hernández-Velázquez, Víctor Manuel
Zamilpa, Alejandro
López-Arellano, María Eugenia
Olmedo-Juárez, Agustín
Higuera-Piedrahita, Rosa Isabel
Delgado-Núñez, Edgar Jesús
González-Cortázar, Manasés
Mendoza-de Gives, Pedro
author_sort Ocampo-Gutiérrez, Ana Yuridia
collection PubMed
description The nematocidal activity of an Oxalis tetraphylla hydroalcoholic extract against the nematode Haemonchus contortus (Hc) was assessed in vitro and the major compounds associated with nematocidal activity were identified. One hydroalcoholic extract was obtained from O. tetraphylla stems and leaves (Ot HE-SLE). The in vitro lethal concentrations (LC50 and LC90) against both eggs and exsheathed Hc infective larvae (L3) were assessed. Ot HE-SLE showed a potent ovicidal activity (LC50 = 0.213 mg/mL; LC90 = 0.71 mg/mL) and larvicidal effect (LC50 = 28.01 mg/mL; LC90 = 69.3 mg/mL). Later on, the extract was bipartitioned to obtain an ethyl acetate phase (EtOAc-Ph) and an aqueous phase (Aq-Ph). Both phases were assessed against Hc eggs at 0.25 and 1.0 mg/mL concentrations. The results with EtOAc-Ph showed 93.6% ovicidal activity, while 96.6% was recorded with Aq-Ph at 48 h post-confrontation (PC). In the case of larvicidal activity, both phases were assessed at 28 mg/mL; Aq-Ph showed >80% larvicidal activity 24 and 72 h PC, while EtOAc-Ph did not show important activity. HPLC analysis showed the presence of coumaric acid and flavonols. Flavonol compounds were the major compounds and were associated with the nematocidal activity. Additionally, the Aq-Ph that showed the highest activity was purified, and the fraction F3 showed the highest nematocidal activity.
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spelling pubmed-95031732022-09-24 Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus Ocampo-Gutiérrez, Ana Yuridia Hernández-Velázquez, Víctor Manuel Zamilpa, Alejandro López-Arellano, María Eugenia Olmedo-Juárez, Agustín Higuera-Piedrahita, Rosa Isabel Delgado-Núñez, Edgar Jesús González-Cortázar, Manasés Mendoza-de Gives, Pedro Pathogens Article The nematocidal activity of an Oxalis tetraphylla hydroalcoholic extract against the nematode Haemonchus contortus (Hc) was assessed in vitro and the major compounds associated with nematocidal activity were identified. One hydroalcoholic extract was obtained from O. tetraphylla stems and leaves (Ot HE-SLE). The in vitro lethal concentrations (LC50 and LC90) against both eggs and exsheathed Hc infective larvae (L3) were assessed. Ot HE-SLE showed a potent ovicidal activity (LC50 = 0.213 mg/mL; LC90 = 0.71 mg/mL) and larvicidal effect (LC50 = 28.01 mg/mL; LC90 = 69.3 mg/mL). Later on, the extract was bipartitioned to obtain an ethyl acetate phase (EtOAc-Ph) and an aqueous phase (Aq-Ph). Both phases were assessed against Hc eggs at 0.25 and 1.0 mg/mL concentrations. The results with EtOAc-Ph showed 93.6% ovicidal activity, while 96.6% was recorded with Aq-Ph at 48 h post-confrontation (PC). In the case of larvicidal activity, both phases were assessed at 28 mg/mL; Aq-Ph showed >80% larvicidal activity 24 and 72 h PC, while EtOAc-Ph did not show important activity. HPLC analysis showed the presence of coumaric acid and flavonols. Flavonol compounds were the major compounds and were associated with the nematocidal activity. Additionally, the Aq-Ph that showed the highest activity was purified, and the fraction F3 showed the highest nematocidal activity. MDPI 2022-09-08 /pmc/articles/PMC9503173/ /pubmed/36145456 http://dx.doi.org/10.3390/pathogens11091024 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ocampo-Gutiérrez, Ana Yuridia
Hernández-Velázquez, Víctor Manuel
Zamilpa, Alejandro
López-Arellano, María Eugenia
Olmedo-Juárez, Agustín
Higuera-Piedrahita, Rosa Isabel
Delgado-Núñez, Edgar Jesús
González-Cortázar, Manasés
Mendoza-de Gives, Pedro
Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus
title Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus
title_full Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus
title_fullStr Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus
title_full_unstemmed Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus
title_short Oxalis tetraphylla (Class: Magnoliopsidae) Possess Flavonoid Phytoconstituents with Nematocidal Activity against Haemonchus contortus
title_sort oxalis tetraphylla (class: magnoliopsidae) possess flavonoid phytoconstituents with nematocidal activity against haemonchus contortus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503173/
https://www.ncbi.nlm.nih.gov/pubmed/36145456
http://dx.doi.org/10.3390/pathogens11091024
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