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Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused huge social and economic distress. Given its rapid spread and the lack of specific treatment options, SARS-CoV-2 needs to be inactivated according to strict biosafety measures during laboratory diagnostics and vaccine developmen...

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Autores principales: Yu, Shouzhi, Wei, Yangyang, Liang, Hongyang, Ji, Wenheng, Chang, Zhen, Xie, Siman, Wang, Yichuan, Li, Wanli, Liu, Yingwei, Wu, Hao, Li, Jie, Wang, Hui, Yang, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503440/
https://www.ncbi.nlm.nih.gov/pubmed/36146745
http://dx.doi.org/10.3390/v14091938
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author Yu, Shouzhi
Wei, Yangyang
Liang, Hongyang
Ji, Wenheng
Chang, Zhen
Xie, Siman
Wang, Yichuan
Li, Wanli
Liu, Yingwei
Wu, Hao
Li, Jie
Wang, Hui
Yang, Xiaoming
author_facet Yu, Shouzhi
Wei, Yangyang
Liang, Hongyang
Ji, Wenheng
Chang, Zhen
Xie, Siman
Wang, Yichuan
Li, Wanli
Liu, Yingwei
Wu, Hao
Li, Jie
Wang, Hui
Yang, Xiaoming
author_sort Yu, Shouzhi
collection PubMed
description Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused huge social and economic distress. Given its rapid spread and the lack of specific treatment options, SARS-CoV-2 needs to be inactivated according to strict biosafety measures during laboratory diagnostics and vaccine development. The inactivation method for SARS-CoV-2 affects research related to the natural virus and its immune activity as an antigen in vaccines. In this study, we used size exclusion chromatography, western blotting, ELISA, an electron microscope, dynamic light scattering, circular dichroism, and surface plasmon resonance to evaluate the effects of four different chemical inactivation methods on the physical and biochemical characterization of SARS-CoV-2. Formaldehyde and β-propiolactone (BPL) treatment can completely inactivate the virus and have no significant effects on the morphology of the virus. None of the four tested inactivation methods affected the secondary structure of the virus, including the α-helix, antiparallel β-sheet, parallel β-sheet, β-turn, and random coil. However, formaldehyde and long-term BPL treatment (48 h) resulted in decreased viral S protein content and increased viral particle aggregation, respectively. The BPL treatment for 24 h can completely inactivate SARS-CoV-2 with the maximum retention of the morphology, physical properties, and the biochemical properties of the potential antigens of the virus. In summary, we have established a characterization system for the comprehensive evaluation of virus inactivation technology, which has important guiding significance for the development of vaccines against SARS-CoV-2 variants and research on natural SARS-CoV-2.
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spelling pubmed-95034402022-09-24 Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments Yu, Shouzhi Wei, Yangyang Liang, Hongyang Ji, Wenheng Chang, Zhen Xie, Siman Wang, Yichuan Li, Wanli Liu, Yingwei Wu, Hao Li, Jie Wang, Hui Yang, Xiaoming Viruses Article Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused huge social and economic distress. Given its rapid spread and the lack of specific treatment options, SARS-CoV-2 needs to be inactivated according to strict biosafety measures during laboratory diagnostics and vaccine development. The inactivation method for SARS-CoV-2 affects research related to the natural virus and its immune activity as an antigen in vaccines. In this study, we used size exclusion chromatography, western blotting, ELISA, an electron microscope, dynamic light scattering, circular dichroism, and surface plasmon resonance to evaluate the effects of four different chemical inactivation methods on the physical and biochemical characterization of SARS-CoV-2. Formaldehyde and β-propiolactone (BPL) treatment can completely inactivate the virus and have no significant effects on the morphology of the virus. None of the four tested inactivation methods affected the secondary structure of the virus, including the α-helix, antiparallel β-sheet, parallel β-sheet, β-turn, and random coil. However, formaldehyde and long-term BPL treatment (48 h) resulted in decreased viral S protein content and increased viral particle aggregation, respectively. The BPL treatment for 24 h can completely inactivate SARS-CoV-2 with the maximum retention of the morphology, physical properties, and the biochemical properties of the potential antigens of the virus. In summary, we have established a characterization system for the comprehensive evaluation of virus inactivation technology, which has important guiding significance for the development of vaccines against SARS-CoV-2 variants and research on natural SARS-CoV-2. MDPI 2022-08-31 /pmc/articles/PMC9503440/ /pubmed/36146745 http://dx.doi.org/10.3390/v14091938 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yu, Shouzhi
Wei, Yangyang
Liang, Hongyang
Ji, Wenheng
Chang, Zhen
Xie, Siman
Wang, Yichuan
Li, Wanli
Liu, Yingwei
Wu, Hao
Li, Jie
Wang, Hui
Yang, Xiaoming
Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments
title Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments
title_full Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments
title_fullStr Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments
title_full_unstemmed Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments
title_short Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments
title_sort comparison of physical and biochemical characterizations of sars-cov-2 inactivated by different treatments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503440/
https://www.ncbi.nlm.nih.gov/pubmed/36146745
http://dx.doi.org/10.3390/v14091938
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