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Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused huge social and economic distress. Given its rapid spread and the lack of specific treatment options, SARS-CoV-2 needs to be inactivated according to strict biosafety measures during laboratory diagnostics and vaccine developmen...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503440/ https://www.ncbi.nlm.nih.gov/pubmed/36146745 http://dx.doi.org/10.3390/v14091938 |
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author | Yu, Shouzhi Wei, Yangyang Liang, Hongyang Ji, Wenheng Chang, Zhen Xie, Siman Wang, Yichuan Li, Wanli Liu, Yingwei Wu, Hao Li, Jie Wang, Hui Yang, Xiaoming |
author_facet | Yu, Shouzhi Wei, Yangyang Liang, Hongyang Ji, Wenheng Chang, Zhen Xie, Siman Wang, Yichuan Li, Wanli Liu, Yingwei Wu, Hao Li, Jie Wang, Hui Yang, Xiaoming |
author_sort | Yu, Shouzhi |
collection | PubMed |
description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused huge social and economic distress. Given its rapid spread and the lack of specific treatment options, SARS-CoV-2 needs to be inactivated according to strict biosafety measures during laboratory diagnostics and vaccine development. The inactivation method for SARS-CoV-2 affects research related to the natural virus and its immune activity as an antigen in vaccines. In this study, we used size exclusion chromatography, western blotting, ELISA, an electron microscope, dynamic light scattering, circular dichroism, and surface plasmon resonance to evaluate the effects of four different chemical inactivation methods on the physical and biochemical characterization of SARS-CoV-2. Formaldehyde and β-propiolactone (BPL) treatment can completely inactivate the virus and have no significant effects on the morphology of the virus. None of the four tested inactivation methods affected the secondary structure of the virus, including the α-helix, antiparallel β-sheet, parallel β-sheet, β-turn, and random coil. However, formaldehyde and long-term BPL treatment (48 h) resulted in decreased viral S protein content and increased viral particle aggregation, respectively. The BPL treatment for 24 h can completely inactivate SARS-CoV-2 with the maximum retention of the morphology, physical properties, and the biochemical properties of the potential antigens of the virus. In summary, we have established a characterization system for the comprehensive evaluation of virus inactivation technology, which has important guiding significance for the development of vaccines against SARS-CoV-2 variants and research on natural SARS-CoV-2. |
format | Online Article Text |
id | pubmed-9503440 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95034402022-09-24 Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments Yu, Shouzhi Wei, Yangyang Liang, Hongyang Ji, Wenheng Chang, Zhen Xie, Siman Wang, Yichuan Li, Wanli Liu, Yingwei Wu, Hao Li, Jie Wang, Hui Yang, Xiaoming Viruses Article Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused huge social and economic distress. Given its rapid spread and the lack of specific treatment options, SARS-CoV-2 needs to be inactivated according to strict biosafety measures during laboratory diagnostics and vaccine development. The inactivation method for SARS-CoV-2 affects research related to the natural virus and its immune activity as an antigen in vaccines. In this study, we used size exclusion chromatography, western blotting, ELISA, an electron microscope, dynamic light scattering, circular dichroism, and surface plasmon resonance to evaluate the effects of four different chemical inactivation methods on the physical and biochemical characterization of SARS-CoV-2. Formaldehyde and β-propiolactone (BPL) treatment can completely inactivate the virus and have no significant effects on the morphology of the virus. None of the four tested inactivation methods affected the secondary structure of the virus, including the α-helix, antiparallel β-sheet, parallel β-sheet, β-turn, and random coil. However, formaldehyde and long-term BPL treatment (48 h) resulted in decreased viral S protein content and increased viral particle aggregation, respectively. The BPL treatment for 24 h can completely inactivate SARS-CoV-2 with the maximum retention of the morphology, physical properties, and the biochemical properties of the potential antigens of the virus. In summary, we have established a characterization system for the comprehensive evaluation of virus inactivation technology, which has important guiding significance for the development of vaccines against SARS-CoV-2 variants and research on natural SARS-CoV-2. MDPI 2022-08-31 /pmc/articles/PMC9503440/ /pubmed/36146745 http://dx.doi.org/10.3390/v14091938 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yu, Shouzhi Wei, Yangyang Liang, Hongyang Ji, Wenheng Chang, Zhen Xie, Siman Wang, Yichuan Li, Wanli Liu, Yingwei Wu, Hao Li, Jie Wang, Hui Yang, Xiaoming Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments |
title | Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments |
title_full | Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments |
title_fullStr | Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments |
title_full_unstemmed | Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments |
title_short | Comparison of Physical and Biochemical Characterizations of SARS-CoV-2 Inactivated by Different Treatments |
title_sort | comparison of physical and biochemical characterizations of sars-cov-2 inactivated by different treatments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503440/ https://www.ncbi.nlm.nih.gov/pubmed/36146745 http://dx.doi.org/10.3390/v14091938 |
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