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Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish

Cyhalofop-butyl (CyB) is a herbicide widely used in paddy fields that may transfer to aquatic ecosystems and cause harm to aquatic organisms. In this study, zebrafish (Danio rerio) were exposed to CyB at environmental concentrations (0.1, 1 and 10 µg/L) throughout their adult life cycle, from embryo...

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Autores principales: Duan, Manman, Guo, Xuanjun, Chen, Xiangguang, Guo, Mengyu, Xu, Hao, Hao, Lubo, Wang, Chengju, Yang, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503539/
https://www.ncbi.nlm.nih.gov/pubmed/36136460
http://dx.doi.org/10.3390/toxics10090495
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author Duan, Manman
Guo, Xuanjun
Chen, Xiangguang
Guo, Mengyu
Xu, Hao
Hao, Lubo
Wang, Chengju
Yang, Yang
author_facet Duan, Manman
Guo, Xuanjun
Chen, Xiangguang
Guo, Mengyu
Xu, Hao
Hao, Lubo
Wang, Chengju
Yang, Yang
author_sort Duan, Manman
collection PubMed
description Cyhalofop-butyl (CyB) is a herbicide widely used in paddy fields that may transfer to aquatic ecosystems and cause harm to aquatic organisms. In this study, zebrafish (Danio rerio) were exposed to CyB at environmental concentrations (0.1, 1 and 10 µg/L) throughout their adult life cycle, from embryo to sexual maturity. The effects of CyB on zebrafish growth and reproduction were studied. It was found that female spawning was inhibited, and adult male fertility decreased. In addition, we examined the expression of sex steroid hormones and genes related to the hypothalamus–pituitary–gonad–liver (HPGL) axis. After 150 days of exposure, the hormone balance in zebrafish was disturbed, and the concentrations of 17β-estradiol (E2) and vitellogenin (VTG) were decreased. Changes in sex hormone were regulated by the expression of genes related to the HPGL axis. These results confirmed that long-term exposure to CyB at environmental concentrations can damage the reproductive capacity of zebrafish by disrupting the transcription of genes related to the HPGL axis. Overall, these data may provide a new understanding of the reproductive toxicity of long-term exposure to CyB in zebrafish parents and offspring.
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spelling pubmed-95035392022-09-24 Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish Duan, Manman Guo, Xuanjun Chen, Xiangguang Guo, Mengyu Xu, Hao Hao, Lubo Wang, Chengju Yang, Yang Toxics Article Cyhalofop-butyl (CyB) is a herbicide widely used in paddy fields that may transfer to aquatic ecosystems and cause harm to aquatic organisms. In this study, zebrafish (Danio rerio) were exposed to CyB at environmental concentrations (0.1, 1 and 10 µg/L) throughout their adult life cycle, from embryo to sexual maturity. The effects of CyB on zebrafish growth and reproduction were studied. It was found that female spawning was inhibited, and adult male fertility decreased. In addition, we examined the expression of sex steroid hormones and genes related to the hypothalamus–pituitary–gonad–liver (HPGL) axis. After 150 days of exposure, the hormone balance in zebrafish was disturbed, and the concentrations of 17β-estradiol (E2) and vitellogenin (VTG) were decreased. Changes in sex hormone were regulated by the expression of genes related to the HPGL axis. These results confirmed that long-term exposure to CyB at environmental concentrations can damage the reproductive capacity of zebrafish by disrupting the transcription of genes related to the HPGL axis. Overall, these data may provide a new understanding of the reproductive toxicity of long-term exposure to CyB in zebrafish parents and offspring. MDPI 2022-08-25 /pmc/articles/PMC9503539/ /pubmed/36136460 http://dx.doi.org/10.3390/toxics10090495 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Duan, Manman
Guo, Xuanjun
Chen, Xiangguang
Guo, Mengyu
Xu, Hao
Hao, Lubo
Wang, Chengju
Yang, Yang
Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish
title Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish
title_full Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish
title_fullStr Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish
title_full_unstemmed Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish
title_short Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish
title_sort life cycle exposure to cyhalofop-butyl induced reproductive toxicity in zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503539/
https://www.ncbi.nlm.nih.gov/pubmed/36136460
http://dx.doi.org/10.3390/toxics10090495
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