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Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish
Cyhalofop-butyl (CyB) is a herbicide widely used in paddy fields that may transfer to aquatic ecosystems and cause harm to aquatic organisms. In this study, zebrafish (Danio rerio) were exposed to CyB at environmental concentrations (0.1, 1 and 10 µg/L) throughout their adult life cycle, from embryo...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503539/ https://www.ncbi.nlm.nih.gov/pubmed/36136460 http://dx.doi.org/10.3390/toxics10090495 |
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author | Duan, Manman Guo, Xuanjun Chen, Xiangguang Guo, Mengyu Xu, Hao Hao, Lubo Wang, Chengju Yang, Yang |
author_facet | Duan, Manman Guo, Xuanjun Chen, Xiangguang Guo, Mengyu Xu, Hao Hao, Lubo Wang, Chengju Yang, Yang |
author_sort | Duan, Manman |
collection | PubMed |
description | Cyhalofop-butyl (CyB) is a herbicide widely used in paddy fields that may transfer to aquatic ecosystems and cause harm to aquatic organisms. In this study, zebrafish (Danio rerio) were exposed to CyB at environmental concentrations (0.1, 1 and 10 µg/L) throughout their adult life cycle, from embryo to sexual maturity. The effects of CyB on zebrafish growth and reproduction were studied. It was found that female spawning was inhibited, and adult male fertility decreased. In addition, we examined the expression of sex steroid hormones and genes related to the hypothalamus–pituitary–gonad–liver (HPGL) axis. After 150 days of exposure, the hormone balance in zebrafish was disturbed, and the concentrations of 17β-estradiol (E2) and vitellogenin (VTG) were decreased. Changes in sex hormone were regulated by the expression of genes related to the HPGL axis. These results confirmed that long-term exposure to CyB at environmental concentrations can damage the reproductive capacity of zebrafish by disrupting the transcription of genes related to the HPGL axis. Overall, these data may provide a new understanding of the reproductive toxicity of long-term exposure to CyB in zebrafish parents and offspring. |
format | Online Article Text |
id | pubmed-9503539 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95035392022-09-24 Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish Duan, Manman Guo, Xuanjun Chen, Xiangguang Guo, Mengyu Xu, Hao Hao, Lubo Wang, Chengju Yang, Yang Toxics Article Cyhalofop-butyl (CyB) is a herbicide widely used in paddy fields that may transfer to aquatic ecosystems and cause harm to aquatic organisms. In this study, zebrafish (Danio rerio) were exposed to CyB at environmental concentrations (0.1, 1 and 10 µg/L) throughout their adult life cycle, from embryo to sexual maturity. The effects of CyB on zebrafish growth and reproduction were studied. It was found that female spawning was inhibited, and adult male fertility decreased. In addition, we examined the expression of sex steroid hormones and genes related to the hypothalamus–pituitary–gonad–liver (HPGL) axis. After 150 days of exposure, the hormone balance in zebrafish was disturbed, and the concentrations of 17β-estradiol (E2) and vitellogenin (VTG) were decreased. Changes in sex hormone were regulated by the expression of genes related to the HPGL axis. These results confirmed that long-term exposure to CyB at environmental concentrations can damage the reproductive capacity of zebrafish by disrupting the transcription of genes related to the HPGL axis. Overall, these data may provide a new understanding of the reproductive toxicity of long-term exposure to CyB in zebrafish parents and offspring. MDPI 2022-08-25 /pmc/articles/PMC9503539/ /pubmed/36136460 http://dx.doi.org/10.3390/toxics10090495 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Duan, Manman Guo, Xuanjun Chen, Xiangguang Guo, Mengyu Xu, Hao Hao, Lubo Wang, Chengju Yang, Yang Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish |
title | Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish |
title_full | Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish |
title_fullStr | Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish |
title_full_unstemmed | Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish |
title_short | Life Cycle Exposure to Cyhalofop-Butyl Induced Reproductive Toxicity in Zebrafish |
title_sort | life cycle exposure to cyhalofop-butyl induced reproductive toxicity in zebrafish |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503539/ https://www.ncbi.nlm.nih.gov/pubmed/36136460 http://dx.doi.org/10.3390/toxics10090495 |
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