Cargando…

The Relationship of Tumor Microbiome and Oral Bacteria and Intestinal Dysbiosis in Canine Mammary Tumor

Canine mammary tumor (CMT) is the most common tumor in dogs, with 50% of malignant cases, and lacks an effective therapeutic schedule, hence its early diagnosis is of great importance to achieve a good prognosis. Microbiota is believed to play important roles in systemic diseases, including cancers....

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Hui-Hua, Du, Chong-Tao, Yu, Chao, Tang, Xin-Yue, Huang, Rong-Lei, Zhang, Yu-Zhu, Gao, Wei, Xie, Guang-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503607/
https://www.ncbi.nlm.nih.gov/pubmed/36142841
http://dx.doi.org/10.3390/ijms231810928
_version_ 1784796006080053248
author Zheng, Hui-Hua
Du, Chong-Tao
Yu, Chao
Tang, Xin-Yue
Huang, Rong-Lei
Zhang, Yu-Zhu
Gao, Wei
Xie, Guang-Hong
author_facet Zheng, Hui-Hua
Du, Chong-Tao
Yu, Chao
Tang, Xin-Yue
Huang, Rong-Lei
Zhang, Yu-Zhu
Gao, Wei
Xie, Guang-Hong
author_sort Zheng, Hui-Hua
collection PubMed
description Canine mammary tumor (CMT) is the most common tumor in dogs, with 50% of malignant cases, and lacks an effective therapeutic schedule, hence its early diagnosis is of great importance to achieve a good prognosis. Microbiota is believed to play important roles in systemic diseases, including cancers. In this study, 91 tumors, 21 oral and fecal samples in total were collected from dogs with CMTs, and 31 oral and 21 fecal samples from healthy dogs were collected as control. The intratumoral, oral and gut bacterial community of dogs with CMTs and healthy dogs was profiled by 16S rRNA high-throughput sequencing and bioinformatic methods. The predominant intratumoral microbes were Ralstonia, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Pseudomonas, unidentified_Chloroplast and Bacteroides at the genus level. In addition, our findings demonstrated striking changes in the composition of the oral and gut bacterium community in the dogs suffered from CMTs compared to the healthy dogs, with a significant increase of Bacteroides which also was the significant microbial biomarker in the oral and gut bacterium community. It showed that the Bacteroides was shared in the intratumoral, oral and intestinal bacterial microbiomes, confirming that microbiota might travel from the mouth to the intestine and finally to the distant mammary tumor tissue. This study provides a new microbiological idea for the treatment of canine mammary tumors, and also provides a theoretical basis for the study of human breast cancer.
format Online
Article
Text
id pubmed-9503607
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95036072022-09-24 The Relationship of Tumor Microbiome and Oral Bacteria and Intestinal Dysbiosis in Canine Mammary Tumor Zheng, Hui-Hua Du, Chong-Tao Yu, Chao Tang, Xin-Yue Huang, Rong-Lei Zhang, Yu-Zhu Gao, Wei Xie, Guang-Hong Int J Mol Sci Article Canine mammary tumor (CMT) is the most common tumor in dogs, with 50% of malignant cases, and lacks an effective therapeutic schedule, hence its early diagnosis is of great importance to achieve a good prognosis. Microbiota is believed to play important roles in systemic diseases, including cancers. In this study, 91 tumors, 21 oral and fecal samples in total were collected from dogs with CMTs, and 31 oral and 21 fecal samples from healthy dogs were collected as control. The intratumoral, oral and gut bacterial community of dogs with CMTs and healthy dogs was profiled by 16S rRNA high-throughput sequencing and bioinformatic methods. The predominant intratumoral microbes were Ralstonia, Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, Pseudomonas, unidentified_Chloroplast and Bacteroides at the genus level. In addition, our findings demonstrated striking changes in the composition of the oral and gut bacterium community in the dogs suffered from CMTs compared to the healthy dogs, with a significant increase of Bacteroides which also was the significant microbial biomarker in the oral and gut bacterium community. It showed that the Bacteroides was shared in the intratumoral, oral and intestinal bacterial microbiomes, confirming that microbiota might travel from the mouth to the intestine and finally to the distant mammary tumor tissue. This study provides a new microbiological idea for the treatment of canine mammary tumors, and also provides a theoretical basis for the study of human breast cancer. MDPI 2022-09-18 /pmc/articles/PMC9503607/ /pubmed/36142841 http://dx.doi.org/10.3390/ijms231810928 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Hui-Hua
Du, Chong-Tao
Yu, Chao
Tang, Xin-Yue
Huang, Rong-Lei
Zhang, Yu-Zhu
Gao, Wei
Xie, Guang-Hong
The Relationship of Tumor Microbiome and Oral Bacteria and Intestinal Dysbiosis in Canine Mammary Tumor
title The Relationship of Tumor Microbiome and Oral Bacteria and Intestinal Dysbiosis in Canine Mammary Tumor
title_full The Relationship of Tumor Microbiome and Oral Bacteria and Intestinal Dysbiosis in Canine Mammary Tumor
title_fullStr The Relationship of Tumor Microbiome and Oral Bacteria and Intestinal Dysbiosis in Canine Mammary Tumor
title_full_unstemmed The Relationship of Tumor Microbiome and Oral Bacteria and Intestinal Dysbiosis in Canine Mammary Tumor
title_short The Relationship of Tumor Microbiome and Oral Bacteria and Intestinal Dysbiosis in Canine Mammary Tumor
title_sort relationship of tumor microbiome and oral bacteria and intestinal dysbiosis in canine mammary tumor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503607/
https://www.ncbi.nlm.nih.gov/pubmed/36142841
http://dx.doi.org/10.3390/ijms231810928
work_keys_str_mv AT zhenghuihua therelationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT duchongtao therelationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT yuchao therelationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT tangxinyue therelationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT huangronglei therelationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT zhangyuzhu therelationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT gaowei therelationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT xieguanghong therelationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT zhenghuihua relationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT duchongtao relationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT yuchao relationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT tangxinyue relationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT huangronglei relationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT zhangyuzhu relationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT gaowei relationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor
AT xieguanghong relationshipoftumormicrobiomeandoralbacteriaandintestinaldysbiosisincaninemammarytumor