Molecular Cloning and Expression Profiling of CncC in Bactrocera dorsalis Hendel

SIMPLE SUMMARY: We cloned the CncC gene of Bactrocera dorsalis and performed a domain analysis of the protein to clarify the expression levels of the gene in different developmental stages and tissue sites of B. dorsalis and to provide a theoretical basis for further investigating the function of CncC in regulating pesticide resistance in B. dorsalis. ABSTRACT: The cap ‘n’ collar isoform C (CncC) transcription factor is thought to be a regulator associated with antioxidant and detoxification genes that can enhance pest resistance by regulating the expression of detoxification enzyme genes. However, this transcription factor has not been well studied in the important agricultural pest Bactrocera dorsalis. In this study, the cDNA sequence of CncC in B. dorsalis was cloned, and the complete ORF sequence was obtained; it had a sequence length of 3378 bp, encoding a total of 1125 amino acids. Phylogenetic tree analysis showed that B. dorsalis CncC belonged to the CNC family and that its amino acid sequence showed the closest relationship with B. tryoni. The conserved structural region of BdCncC was analyzed and was found to include a conserved bZIP superfamily structural domain. Spatiotemporal expression analysis revealed that BdCncC was most highly expressed in the adult Malpighian tubules, followed by the antennae, foregut, and midgut, and then the brain, hemolymph, hindgut, and fat body. BdCncC was expressed at every developmental stage, and the highest expression was found in mature males. This study provides a theoretical basis for an in-depth investigation of the function of BdCncC in regulating pesticide resistance in B. dorsalis.