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Continuous Circulation of Yellow Fever among Rural Populations in the Central African Republic

Yellow fever remains a public-health threat in remote regions of Africa. Here, we report the identification and genetic characterisation of one yellow-fever case observed during the investigation of a cluster of nine suspected haemorrhagic fever cases in a village in the Central African Republic. Sa...

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Autores principales: SIMO TCHETGNA, Huguette, DESCORPS-DECLERE, Stéphane, SELEKON, Benjamin, GARBA-OUANGOLE, Sandra, KONAMNA, Xavier, SOUNGOUZA, Mathieu, TEKPA, Gaspard, SOMSE, Pierre, NAKOUNE, Emmanuel, BERTHET, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503741/
https://www.ncbi.nlm.nih.gov/pubmed/36146820
http://dx.doi.org/10.3390/v14092014
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author SIMO TCHETGNA, Huguette
DESCORPS-DECLERE, Stéphane
SELEKON, Benjamin
GARBA-OUANGOLE, Sandra
KONAMNA, Xavier
SOUNGOUZA, Mathieu
TEKPA, Gaspard
SOMSE, Pierre
NAKOUNE, Emmanuel
BERTHET, Nicolas
author_facet SIMO TCHETGNA, Huguette
DESCORPS-DECLERE, Stéphane
SELEKON, Benjamin
GARBA-OUANGOLE, Sandra
KONAMNA, Xavier
SOUNGOUZA, Mathieu
TEKPA, Gaspard
SOMSE, Pierre
NAKOUNE, Emmanuel
BERTHET, Nicolas
author_sort SIMO TCHETGNA, Huguette
collection PubMed
description Yellow fever remains a public-health threat in remote regions of Africa. Here, we report the identification and genetic characterisation of one yellow-fever case observed during the investigation of a cluster of nine suspected haemorrhagic fever cases in a village in the Central African Republic. Samples were tested using real-time RT-PCR targeting the main African haemorrhagic fever viruses. Following negative results, we attempted virus isolation on VERO E6 cells and new-born mice and rescreened the samples using rRT-PCR. The whole viral genome was sequenced using an Illumina NovaSeq 6000 sequencer. Yellow-fever virus (YFV) was isolated from one woman who reported farming activities in a forest setting several days before disease onset. Phylogenetic analysis shows that this strain belongs to the East–Central African YFV genotype, with an estimated emergence some 63 years ago. Finally, five unique amino-acid changes are present in the capsid, envelop, NS1A, NS3, and NS4B proteins. More efforts are required to control yellow-fever re-emergence in resource-limited settings.
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spelling pubmed-95037412022-09-24 Continuous Circulation of Yellow Fever among Rural Populations in the Central African Republic SIMO TCHETGNA, Huguette DESCORPS-DECLERE, Stéphane SELEKON, Benjamin GARBA-OUANGOLE, Sandra KONAMNA, Xavier SOUNGOUZA, Mathieu TEKPA, Gaspard SOMSE, Pierre NAKOUNE, Emmanuel BERTHET, Nicolas Viruses Article Yellow fever remains a public-health threat in remote regions of Africa. Here, we report the identification and genetic characterisation of one yellow-fever case observed during the investigation of a cluster of nine suspected haemorrhagic fever cases in a village in the Central African Republic. Samples were tested using real-time RT-PCR targeting the main African haemorrhagic fever viruses. Following negative results, we attempted virus isolation on VERO E6 cells and new-born mice and rescreened the samples using rRT-PCR. The whole viral genome was sequenced using an Illumina NovaSeq 6000 sequencer. Yellow-fever virus (YFV) was isolated from one woman who reported farming activities in a forest setting several days before disease onset. Phylogenetic analysis shows that this strain belongs to the East–Central African YFV genotype, with an estimated emergence some 63 years ago. Finally, five unique amino-acid changes are present in the capsid, envelop, NS1A, NS3, and NS4B proteins. More efforts are required to control yellow-fever re-emergence in resource-limited settings. MDPI 2022-09-12 /pmc/articles/PMC9503741/ /pubmed/36146820 http://dx.doi.org/10.3390/v14092014 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
SIMO TCHETGNA, Huguette
DESCORPS-DECLERE, Stéphane
SELEKON, Benjamin
GARBA-OUANGOLE, Sandra
KONAMNA, Xavier
SOUNGOUZA, Mathieu
TEKPA, Gaspard
SOMSE, Pierre
NAKOUNE, Emmanuel
BERTHET, Nicolas
Continuous Circulation of Yellow Fever among Rural Populations in the Central African Republic
title Continuous Circulation of Yellow Fever among Rural Populations in the Central African Republic
title_full Continuous Circulation of Yellow Fever among Rural Populations in the Central African Republic
title_fullStr Continuous Circulation of Yellow Fever among Rural Populations in the Central African Republic
title_full_unstemmed Continuous Circulation of Yellow Fever among Rural Populations in the Central African Republic
title_short Continuous Circulation of Yellow Fever among Rural Populations in the Central African Republic
title_sort continuous circulation of yellow fever among rural populations in the central african republic
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503741/
https://www.ncbi.nlm.nih.gov/pubmed/36146820
http://dx.doi.org/10.3390/v14092014
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