Sentinel Lymph Node Mapping with Indirect Lymphangiography for Canine Mast Cell Tumour

SIMPLE SUMMARY: Mast cell tumour (MCT) is a common cutaneous and subcutaneous neoplasia in dogs. Recent studies describe that sentinel lymph node (SLN) assessment is more specific to stage MCT, while regional lymph node (RLN) evaluation is not as specific. SNL is the first site of drainage of a tumour and the first metastatic site in several tumours. The study aims to evaluate the SLN drainage mapping of MCT with indirect lymphography in dogs. The second objective of the study is to compare the SLN to the RLN. Survey radiographs followed by an indirect lymphography were obtained for SLN mapping. Twenty-six dogs with 29 MCTs were included. SLNs were detectable in 26 MCTs and radiographic indirect lymphangiography with Lipiodol was able to detect at least one SLN in 90% of MCTs in dogs. In conclusion, radiographic indirect lymphangiography with Lipiodol is a feasible technique to map SLNs and its draining system in MCTs. The lymph drainage pattern of the MCTs may be different for each MCT and more than one SLN can be involved. ABSTRACT: Mast cell tumour (MCT) is a common cutaneous and subcutaneous neoplasia in dogs. It can metastasise to lymph nodes (LNs), and this adversely affects the prognosis and treatment. The study aims to evaluate the SLN mapping of MCTs with radiographic indirect lymphography. Dogs that underwent clinical staging were prospectively enrolled. Lipiodol was injected around the MCT or the surgical scar. After 24 h, LNs that picked up contrast were radiographically assessed. Twenty-six dogs with 29 MCTs were included. MCTs were confirmed histologically, while SLNs were evaluated either by cytology and/or histology. SLNs were detectable in 23 dogs with 26 MCTs. Lymphatic vessels were visible in 19 MCTs. In nine MCTs, at least two SLNs picked up contrast. In particular, seven MCTs involved two SLNs, and two MCTs involved three different SLNs. In nine MCTs, at least a SLN was metastatic. This study indicates that the lymph drainage pattern of the MCTs may be different for each MCT, and more than one SLN can be involved. Indirect lymphangiography with Lipiodol allowed the detection of the SLN in 90% of MCTs. This provided clinically relevant information to remove the LN and stage the patient.