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Modification of erythrocyte membrane phospholipid composition in preterm newborns with retinopathy of prematurity: The omegaROP study

N-3 polyunsaturated fatty acids (PUFAs) may prevent retinal vascular abnormalities observed in oxygen-induced retinopathy, a model of retinopathy of prematurity (ROP). In the OmegaROP prospective cohort study, we showed that preterm infants who will develop ROP accumulate the n-6 PUFA arachidonic ac...

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Autores principales: Karadayi, Rémi, Pallot, Charlotte, Cabaret, Stéphanie, Mazzocco, Julie, Gabrielle, Pierre-Henry, Semama, Denis S., Chantegret, Corinne, Ternoy, Ninon, Martin, Delphine, Donier, Aurélie, Gregoire, Stéphane, Creuzot-Garcher, Catherine P., Bron, Alain M., Bretillon, Lionel, Berdeaux, Olivier, Acar, Niyazi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504055/
https://www.ncbi.nlm.nih.gov/pubmed/36158214
http://dx.doi.org/10.3389/fcell.2022.921691
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author Karadayi, Rémi
Pallot, Charlotte
Cabaret, Stéphanie
Mazzocco, Julie
Gabrielle, Pierre-Henry
Semama, Denis S.
Chantegret, Corinne
Ternoy, Ninon
Martin, Delphine
Donier, Aurélie
Gregoire, Stéphane
Creuzot-Garcher, Catherine P.
Bron, Alain M.
Bretillon, Lionel
Berdeaux, Olivier
Acar, Niyazi
author_facet Karadayi, Rémi
Pallot, Charlotte
Cabaret, Stéphanie
Mazzocco, Julie
Gabrielle, Pierre-Henry
Semama, Denis S.
Chantegret, Corinne
Ternoy, Ninon
Martin, Delphine
Donier, Aurélie
Gregoire, Stéphane
Creuzot-Garcher, Catherine P.
Bron, Alain M.
Bretillon, Lionel
Berdeaux, Olivier
Acar, Niyazi
author_sort Karadayi, Rémi
collection PubMed
description N-3 polyunsaturated fatty acids (PUFAs) may prevent retinal vascular abnormalities observed in oxygen-induced retinopathy, a model of retinopathy of prematurity (ROP). In the OmegaROP prospective cohort study, we showed that preterm infants who will develop ROP accumulate the n-6 PUFA arachidonic acid (ARA) at the expense of the n-3 PUFA docosahexaenoic acid (DHA) in erythrocytes with advancing gestational age (GA). As mice lacking plasmalogens ―That are specific phospholipids considered as reservoirs of n-6 and n-3 PUFAs― Display a ROP-like phenotype, the aim of this study was to determine whether plasmalogens are responsible for the changes observed in subjects from the OmegaROP study. Accordingly, preterm infants aged less than 29 weeks GA were recruited at birth in the Neonatal Intensive Care Unit of University Hospital Dijon, France. Blood was sampled very early after birth to avoid any nutritional influence on its lipid composition. The lipid composition of erythrocytes and the structure of phospholipids including plasmalogens were determined by global lipidomics using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). LC-HRMS data confirmed our previous observations by showing a negative association between the erythrocyte content in phospholipid esterified to n-6 PUFAs and GA in infants without ROP (rho = −0.485, p = 0.013 and rho = −0.477, p = 0.015 for ethanolamine and choline total phospholipids, respectively). Phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) species with ARA, namely PtdCho16:0/20:4 (rho = −0.511, p < 0.01) and PtdEtn18:1/20:4 (rho = −0.479, p = 0.015), were the major contributors to the relationship observed. On the contrary, preterm infants developing ROP displayed negative association between PtdEtn species with n-3 PUFAs and GA (rho = −0.380, p = 0.034). They were also characterized by a positive association between GA and the ratio of ethanolamine plasmalogens (PlsEtn) with n-6 PUFA to PlsEtn with n-3 PUFAs (rho = 0.420, p = 0.029), as well as the ratio of PlsEtn with ARA to PlsEtn with DHA (rho = 0.843, p = 0.011). Altogether, these data confirm the potential accumulation of n-6 PUFAs with advancing GA in erythrocytes of infants developing ROP. These changes may be partly due to plasmalogens.
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spelling pubmed-95040552022-09-24 Modification of erythrocyte membrane phospholipid composition in preterm newborns with retinopathy of prematurity: The omegaROP study Karadayi, Rémi Pallot, Charlotte Cabaret, Stéphanie Mazzocco, Julie Gabrielle, Pierre-Henry Semama, Denis S. Chantegret, Corinne Ternoy, Ninon Martin, Delphine Donier, Aurélie Gregoire, Stéphane Creuzot-Garcher, Catherine P. Bron, Alain M. Bretillon, Lionel Berdeaux, Olivier Acar, Niyazi Front Cell Dev Biol Cell and Developmental Biology N-3 polyunsaturated fatty acids (PUFAs) may prevent retinal vascular abnormalities observed in oxygen-induced retinopathy, a model of retinopathy of prematurity (ROP). In the OmegaROP prospective cohort study, we showed that preterm infants who will develop ROP accumulate the n-6 PUFA arachidonic acid (ARA) at the expense of the n-3 PUFA docosahexaenoic acid (DHA) in erythrocytes with advancing gestational age (GA). As mice lacking plasmalogens ―That are specific phospholipids considered as reservoirs of n-6 and n-3 PUFAs― Display a ROP-like phenotype, the aim of this study was to determine whether plasmalogens are responsible for the changes observed in subjects from the OmegaROP study. Accordingly, preterm infants aged less than 29 weeks GA were recruited at birth in the Neonatal Intensive Care Unit of University Hospital Dijon, France. Blood was sampled very early after birth to avoid any nutritional influence on its lipid composition. The lipid composition of erythrocytes and the structure of phospholipids including plasmalogens were determined by global lipidomics using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). LC-HRMS data confirmed our previous observations by showing a negative association between the erythrocyte content in phospholipid esterified to n-6 PUFAs and GA in infants without ROP (rho = −0.485, p = 0.013 and rho = −0.477, p = 0.015 for ethanolamine and choline total phospholipids, respectively). Phosphatidylcholine (PtdCho) and phosphatidylethanolamine (PtdEtn) species with ARA, namely PtdCho16:0/20:4 (rho = −0.511, p < 0.01) and PtdEtn18:1/20:4 (rho = −0.479, p = 0.015), were the major contributors to the relationship observed. On the contrary, preterm infants developing ROP displayed negative association between PtdEtn species with n-3 PUFAs and GA (rho = −0.380, p = 0.034). They were also characterized by a positive association between GA and the ratio of ethanolamine plasmalogens (PlsEtn) with n-6 PUFA to PlsEtn with n-3 PUFAs (rho = 0.420, p = 0.029), as well as the ratio of PlsEtn with ARA to PlsEtn with DHA (rho = 0.843, p = 0.011). Altogether, these data confirm the potential accumulation of n-6 PUFAs with advancing GA in erythrocytes of infants developing ROP. These changes may be partly due to plasmalogens. Frontiers Media S.A. 2022-09-09 /pmc/articles/PMC9504055/ /pubmed/36158214 http://dx.doi.org/10.3389/fcell.2022.921691 Text en Copyright © 2022 Karadayi, Pallot, Cabaret, Mazzocco, Gabrielle, Semama, Chantegret, Ternoy, Martin, Donier, Gregoire, Creuzot-Garcher, Bron, Bretillon, Berdeaux and Acar. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Karadayi, Rémi
Pallot, Charlotte
Cabaret, Stéphanie
Mazzocco, Julie
Gabrielle, Pierre-Henry
Semama, Denis S.
Chantegret, Corinne
Ternoy, Ninon
Martin, Delphine
Donier, Aurélie
Gregoire, Stéphane
Creuzot-Garcher, Catherine P.
Bron, Alain M.
Bretillon, Lionel
Berdeaux, Olivier
Acar, Niyazi
Modification of erythrocyte membrane phospholipid composition in preterm newborns with retinopathy of prematurity: The omegaROP study
title Modification of erythrocyte membrane phospholipid composition in preterm newborns with retinopathy of prematurity: The omegaROP study
title_full Modification of erythrocyte membrane phospholipid composition in preterm newborns with retinopathy of prematurity: The omegaROP study
title_fullStr Modification of erythrocyte membrane phospholipid composition in preterm newborns with retinopathy of prematurity: The omegaROP study
title_full_unstemmed Modification of erythrocyte membrane phospholipid composition in preterm newborns with retinopathy of prematurity: The omegaROP study
title_short Modification of erythrocyte membrane phospholipid composition in preterm newborns with retinopathy of prematurity: The omegaROP study
title_sort modification of erythrocyte membrane phospholipid composition in preterm newborns with retinopathy of prematurity: the omegarop study
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504055/
https://www.ncbi.nlm.nih.gov/pubmed/36158214
http://dx.doi.org/10.3389/fcell.2022.921691
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