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Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of Pseudomonas aeruginosa PAO1 and PA14
The increase in multidrug-resistant microorganisms represents a global threat requiring the development novel strategies to fight bacterial infection. This study aimed to assess the effect of silver nanoparticles (bio-AgNPs) on bacterial growth, biofilm formation, production of virulence factors, an...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504124/ https://www.ncbi.nlm.nih.gov/pubmed/36144357 http://dx.doi.org/10.3390/microorganisms10091755 |
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author | Saeki, Erika Kushikawa Martins, Heloísa Moreira de Camargo, Larissa Ciappina Anversa, Laís Tavares, Eliandro Reis Yamada-Ogatta, Sueli Fumie Lioni, Lucy Megumi Yamauchi Kobayashi, Renata Katsuko Takayama Nakazato, Gerson |
author_facet | Saeki, Erika Kushikawa Martins, Heloísa Moreira de Camargo, Larissa Ciappina Anversa, Laís Tavares, Eliandro Reis Yamada-Ogatta, Sueli Fumie Lioni, Lucy Megumi Yamauchi Kobayashi, Renata Katsuko Takayama Nakazato, Gerson |
author_sort | Saeki, Erika Kushikawa |
collection | PubMed |
description | The increase in multidrug-resistant microorganisms represents a global threat requiring the development novel strategies to fight bacterial infection. This study aimed to assess the effect of silver nanoparticles (bio-AgNPs) on bacterial growth, biofilm formation, production of virulence factors, and expression of genes related to the quorum-sensing (QS) system of P. aeruginosa PAO1 and PA14. Biofilm formation and virulence assays were performed with bio-AgNPs. RT-qPCR was carried out to determine the effect of bio-AgNPs on the QS regulatory genes lasI, lasR, rhlI, rhlR, pqsA, and mvfR. Bio-AgNPs had an MIC value of 62.50 μM, for both strains. Phenotypic and genotypic assays were carried out using sub-MIC values. Experimental results showed that treatment with sub-MICs of bio-AgNPs reduced (p < 0.05) the motility and rhamnolipids and elastase production in P. aeruginosa PAO1. In PA14, bio-AgNPs stimulated swarming and twitching motilities as well as biofilm formation and elastase and pyocyanin production. Bio-AgNP treatment increased (p < 0.05) the expression of QS genes in PAO1 and PA14. Despite the different phenotypic behaviors in both strains, both showed an increase in the expression of QS genes. Demonstrating that the bio-AgNPs acted in the induction of regulation. The possible mechanism underlying the action of bio-AgNPs involves the induction of the rhl and/or pqs system of PAO1 and of the las and/or pqs system of PA14. These results suggest that exposure to low concentrations of bio-AgNPs may promote the expression of QS regulatory genes in P. aeruginosa, consequently inducing the production of virulence factors such as elastase, pyocyanin, and biofilms. |
format | Online Article Text |
id | pubmed-9504124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95041242022-09-24 Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of Pseudomonas aeruginosa PAO1 and PA14 Saeki, Erika Kushikawa Martins, Heloísa Moreira de Camargo, Larissa Ciappina Anversa, Laís Tavares, Eliandro Reis Yamada-Ogatta, Sueli Fumie Lioni, Lucy Megumi Yamauchi Kobayashi, Renata Katsuko Takayama Nakazato, Gerson Microorganisms Article The increase in multidrug-resistant microorganisms represents a global threat requiring the development novel strategies to fight bacterial infection. This study aimed to assess the effect of silver nanoparticles (bio-AgNPs) on bacterial growth, biofilm formation, production of virulence factors, and expression of genes related to the quorum-sensing (QS) system of P. aeruginosa PAO1 and PA14. Biofilm formation and virulence assays were performed with bio-AgNPs. RT-qPCR was carried out to determine the effect of bio-AgNPs on the QS regulatory genes lasI, lasR, rhlI, rhlR, pqsA, and mvfR. Bio-AgNPs had an MIC value of 62.50 μM, for both strains. Phenotypic and genotypic assays were carried out using sub-MIC values. Experimental results showed that treatment with sub-MICs of bio-AgNPs reduced (p < 0.05) the motility and rhamnolipids and elastase production in P. aeruginosa PAO1. In PA14, bio-AgNPs stimulated swarming and twitching motilities as well as biofilm formation and elastase and pyocyanin production. Bio-AgNP treatment increased (p < 0.05) the expression of QS genes in PAO1 and PA14. Despite the different phenotypic behaviors in both strains, both showed an increase in the expression of QS genes. Demonstrating that the bio-AgNPs acted in the induction of regulation. The possible mechanism underlying the action of bio-AgNPs involves the induction of the rhl and/or pqs system of PAO1 and of the las and/or pqs system of PA14. These results suggest that exposure to low concentrations of bio-AgNPs may promote the expression of QS regulatory genes in P. aeruginosa, consequently inducing the production of virulence factors such as elastase, pyocyanin, and biofilms. MDPI 2022-08-30 /pmc/articles/PMC9504124/ /pubmed/36144357 http://dx.doi.org/10.3390/microorganisms10091755 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Saeki, Erika Kushikawa Martins, Heloísa Moreira de Camargo, Larissa Ciappina Anversa, Laís Tavares, Eliandro Reis Yamada-Ogatta, Sueli Fumie Lioni, Lucy Megumi Yamauchi Kobayashi, Renata Katsuko Takayama Nakazato, Gerson Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of Pseudomonas aeruginosa PAO1 and PA14 |
title | Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of Pseudomonas aeruginosa PAO1 and PA14 |
title_full | Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of Pseudomonas aeruginosa PAO1 and PA14 |
title_fullStr | Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of Pseudomonas aeruginosa PAO1 and PA14 |
title_full_unstemmed | Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of Pseudomonas aeruginosa PAO1 and PA14 |
title_short | Effect of Biogenic Silver Nanoparticles on the Quorum-Sensing System of Pseudomonas aeruginosa PAO1 and PA14 |
title_sort | effect of biogenic silver nanoparticles on the quorum-sensing system of pseudomonas aeruginosa pao1 and pa14 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504124/ https://www.ncbi.nlm.nih.gov/pubmed/36144357 http://dx.doi.org/10.3390/microorganisms10091755 |
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