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New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of Ralstonia solanacearum
Ralstonia solanacearum is one of the globally significant plant pathogens that infect a wide host range of economically important plants. A study was conducted to evaluate the hypothesis that an avirulent strain of R. solanacearum can act as a biocontrol mediator for managing potato bacterial wilt....
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504442/ https://www.ncbi.nlm.nih.gov/pubmed/36144416 http://dx.doi.org/10.3390/microorganisms10091814 |
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author | Moussa, Zeiad Rashad, Ehsan M. Elsherbiny, Elsherbiny A. Al-Askar, Abdulaziz A. Arishi, Amr Abker Al-Otibi, Fatimah O. Saber, WesamEldin I. A. |
author_facet | Moussa, Zeiad Rashad, Ehsan M. Elsherbiny, Elsherbiny A. Al-Askar, Abdulaziz A. Arishi, Amr Abker Al-Otibi, Fatimah O. Saber, WesamEldin I. A. |
author_sort | Moussa, Zeiad |
collection | PubMed |
description | Ralstonia solanacearum is one of the globally significant plant pathogens that infect a wide host range of economically important plants. A study was conducted to evaluate the hypothesis that an avirulent strain of R. solanacearum can act as a biocontrol mediator for managing potato bacterial wilt. Virulent R. solanacearum was isolated and identified (GenBank accession number; OP180100). The avirulent strain was obtained from the virulent strain through storage for 3 weeks until the development of deep red colonies. The virulent strain had higher lytic activity than the avirulent strain. Tubers’ treatments by the avirulent strain of R. solanacearum, (supernatant, boiled supernatant, and dead cells) significantly reduced plant disease rating and increased the growth, physiological activities, and biomass of potato compared to the untreated, infected control. The major components detected by GC–MS in the supernatant revealed 10.86% palmitic acid (virulent), and 18.03% 1,3-dioxolane, 2,4,5-trimethyl- (avirulent), whereas the major component in the boiled supernatant was 2-hydroxy-gamma-butyrolactone in the virulent (21.17%) and avirulent (27.78%) strains. This is the first research that assessed the influence of boiled supernatant and dead cells of virulent and avirulent R. solanacearum strains in controlling bacterial wilt disease. Additional work is encouraged for further elucidation of such a topic. |
format | Online Article Text |
id | pubmed-9504442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95044422022-09-24 New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of Ralstonia solanacearum Moussa, Zeiad Rashad, Ehsan M. Elsherbiny, Elsherbiny A. Al-Askar, Abdulaziz A. Arishi, Amr Abker Al-Otibi, Fatimah O. Saber, WesamEldin I. A. Microorganisms Article Ralstonia solanacearum is one of the globally significant plant pathogens that infect a wide host range of economically important plants. A study was conducted to evaluate the hypothesis that an avirulent strain of R. solanacearum can act as a biocontrol mediator for managing potato bacterial wilt. Virulent R. solanacearum was isolated and identified (GenBank accession number; OP180100). The avirulent strain was obtained from the virulent strain through storage for 3 weeks until the development of deep red colonies. The virulent strain had higher lytic activity than the avirulent strain. Tubers’ treatments by the avirulent strain of R. solanacearum, (supernatant, boiled supernatant, and dead cells) significantly reduced plant disease rating and increased the growth, physiological activities, and biomass of potato compared to the untreated, infected control. The major components detected by GC–MS in the supernatant revealed 10.86% palmitic acid (virulent), and 18.03% 1,3-dioxolane, 2,4,5-trimethyl- (avirulent), whereas the major component in the boiled supernatant was 2-hydroxy-gamma-butyrolactone in the virulent (21.17%) and avirulent (27.78%) strains. This is the first research that assessed the influence of boiled supernatant and dead cells of virulent and avirulent R. solanacearum strains in controlling bacterial wilt disease. Additional work is encouraged for further elucidation of such a topic. MDPI 2022-09-10 /pmc/articles/PMC9504442/ /pubmed/36144416 http://dx.doi.org/10.3390/microorganisms10091814 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Moussa, Zeiad Rashad, Ehsan M. Elsherbiny, Elsherbiny A. Al-Askar, Abdulaziz A. Arishi, Amr Abker Al-Otibi, Fatimah O. Saber, WesamEldin I. A. New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of Ralstonia solanacearum |
title | New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of Ralstonia solanacearum |
title_full | New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of Ralstonia solanacearum |
title_fullStr | New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of Ralstonia solanacearum |
title_full_unstemmed | New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of Ralstonia solanacearum |
title_short | New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of Ralstonia solanacearum |
title_sort | new strategy for inducing resistance against bacterial wilt disease using an avirulent strain of ralstonia solanacearum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504442/ https://www.ncbi.nlm.nih.gov/pubmed/36144416 http://dx.doi.org/10.3390/microorganisms10091814 |
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