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New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1

[Image: see text] Patients who suffer from birch pollinosis often develop adverse reactions to the consumption of fresh apples due to the structural similarity of the allergens Bet v 1 and Mal d 1 from birch and apples, respectively. A different allergenic potential for Mal d 1 isoallergens is postu...

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Autores principales: Kaeswurm, Julia A. H., Straub, Leonie V., Klußmann, Alexandra, Brockmeyer, Jens, Buchweitz, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504475/
https://www.ncbi.nlm.nih.gov/pubmed/36074755
http://dx.doi.org/10.1021/acs.jafc.2c03745
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author Kaeswurm, Julia A. H.
Straub, Leonie V.
Klußmann, Alexandra
Brockmeyer, Jens
Buchweitz, Maria
author_facet Kaeswurm, Julia A. H.
Straub, Leonie V.
Klußmann, Alexandra
Brockmeyer, Jens
Buchweitz, Maria
author_sort Kaeswurm, Julia A. H.
collection PubMed
description [Image: see text] Patients who suffer from birch pollinosis often develop adverse reactions to the consumption of fresh apples due to the structural similarity of the allergens Bet v 1 and Mal d 1 from birch and apples, respectively. A different allergenic potential for Mal d 1 isoallergens is postulated, but approaches to quantify the Mal d 1 isoallergen-specific are missing. Therefore, a bottom-up proteomics approach was developed to quantify Mal d 1 by stable isotope dilution and microHPLC-QTOF analyses. Marker peptides for individual isoallergens (Mal d 1.01–Mal d 1.03 and Mal d 1.06), combinations thereof (Mal d 1.01 + 1.02, Mal d 1.02 + 1.06, and Mal d 1.04 + 1.05), and two global marker peptides, comprising Mal d 1.01 + 1.02 + 1.04 + 1.05 and Mal d 1.03 + 1.06 + 1.07 + 1.08 + 1.09, were identified. By the use of an extraction standard (r-Mal d 1_mut), an optimized protocol for extraction and tryptic digestion of apple proteins was developed, and the variety-specific extraction efficiency was monitored for the flesh and peel of apples. The Mal d 1 contents in flesh and peel of five commercial apple breeds and four apple varieties from orchard meadows were quantified isoallergen-specific.
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spelling pubmed-95044752023-09-08 New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1 Kaeswurm, Julia A. H. Straub, Leonie V. Klußmann, Alexandra Brockmeyer, Jens Buchweitz, Maria J Agric Food Chem [Image: see text] Patients who suffer from birch pollinosis often develop adverse reactions to the consumption of fresh apples due to the structural similarity of the allergens Bet v 1 and Mal d 1 from birch and apples, respectively. A different allergenic potential for Mal d 1 isoallergens is postulated, but approaches to quantify the Mal d 1 isoallergen-specific are missing. Therefore, a bottom-up proteomics approach was developed to quantify Mal d 1 by stable isotope dilution and microHPLC-QTOF analyses. Marker peptides for individual isoallergens (Mal d 1.01–Mal d 1.03 and Mal d 1.06), combinations thereof (Mal d 1.01 + 1.02, Mal d 1.02 + 1.06, and Mal d 1.04 + 1.05), and two global marker peptides, comprising Mal d 1.01 + 1.02 + 1.04 + 1.05 and Mal d 1.03 + 1.06 + 1.07 + 1.08 + 1.09, were identified. By the use of an extraction standard (r-Mal d 1_mut), an optimized protocol for extraction and tryptic digestion of apple proteins was developed, and the variety-specific extraction efficiency was monitored for the flesh and peel of apples. The Mal d 1 contents in flesh and peel of five commercial apple breeds and four apple varieties from orchard meadows were quantified isoallergen-specific. American Chemical Society 2022-09-08 2022-09-21 /pmc/articles/PMC9504475/ /pubmed/36074755 http://dx.doi.org/10.1021/acs.jafc.2c03745 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Kaeswurm, Julia A. H.
Straub, Leonie V.
Klußmann, Alexandra
Brockmeyer, Jens
Buchweitz, Maria
New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1
title New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1
title_full New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1
title_fullStr New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1
title_full_unstemmed New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1
title_short New Mass Spectrometric Approach to Quantify the Major Isoallergens of the Apple Allergen Mal d 1
title_sort new mass spectrometric approach to quantify the major isoallergens of the apple allergen mal d 1
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504475/
https://www.ncbi.nlm.nih.gov/pubmed/36074755
http://dx.doi.org/10.1021/acs.jafc.2c03745
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