Cargando…

The microbiota changes of the brown dog tick, Rhipicephalus sanguineus under starvation stress

Rhipicephalus sanguineus, the brown dog tick, is the most widespread tick in the world and a predominant vector of multiple pathogens affecting wild and domestic animals. There is an increasing interest in understanding the role of tick microbiome in pathogen acquisition and transmission as well as...

Descripción completa

Detalles Bibliográficos
Autores principales: An, Liping, Bhowmick, Biswajit, Liang, Dejuan, Suo, Penghui, Liao, Chenghong, Zhao, Jianguo, Han, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504665/
https://www.ncbi.nlm.nih.gov/pubmed/36160860
http://dx.doi.org/10.3389/fphys.2022.932130
_version_ 1784796273843372032
author An, Liping
Bhowmick, Biswajit
Liang, Dejuan
Suo, Penghui
Liao, Chenghong
Zhao, Jianguo
Han, Qian
author_facet An, Liping
Bhowmick, Biswajit
Liang, Dejuan
Suo, Penghui
Liao, Chenghong
Zhao, Jianguo
Han, Qian
author_sort An, Liping
collection PubMed
description Rhipicephalus sanguineus, the brown dog tick, is the most widespread tick in the world and a predominant vector of multiple pathogens affecting wild and domestic animals. There is an increasing interest in understanding the role of tick microbiome in pathogen acquisition and transmission as well as in environment–vector interfaces. Several studies suggested that the tick microbial communities are under the influence of several factors including the tick species, dietary bloodmeal, and physiological stress. Compared with insects, very little of the microbial community is known to contribute to the nutrition of the host. Therefore, it is of significance to elucidate the regulation of the microbial community of Rh. Sanguineus under starvation stress. Starvation stress was induced in wild-type adults (1 month, 2 months, 4 months, 6 months) and the microbial composition and diversity were analyzed before and after blood feeding. After the evaluation, it was found that the microbial community composition of Rh. sanguineus changed significantly with starvation stress. The dominant symbiotic bacteria Coxiella spp. of Rh. sanguineus gradually decreased with the prolongation of starvation stress. We also demonstrated that the starvation tolerance of Rh. sanguineus was as long as 6 months. Next, Coxiella-like endosymbionts were quantitatively analyzed by fluorescence quantitative PCR. We found a pronounced tissue tropism in the Malpighian tubule and female gonad, and less in the midgut and salivary gland organs. Finally, the blood-fed nymphs were injected with ofloxacin within 24 h. The nymphs were allowed to develop into adults. It was found that the adult blood-sucking rate, adult weight after blood meal, fecundity (egg hatching rate), and feeding period of the newly hatched larvae were all affected to varying degrees, indicating that the removal of most symbiotic bacteria had an irreversible effect on it.
format Online
Article
Text
id pubmed-9504665
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95046652022-09-24 The microbiota changes of the brown dog tick, Rhipicephalus sanguineus under starvation stress An, Liping Bhowmick, Biswajit Liang, Dejuan Suo, Penghui Liao, Chenghong Zhao, Jianguo Han, Qian Front Physiol Physiology Rhipicephalus sanguineus, the brown dog tick, is the most widespread tick in the world and a predominant vector of multiple pathogens affecting wild and domestic animals. There is an increasing interest in understanding the role of tick microbiome in pathogen acquisition and transmission as well as in environment–vector interfaces. Several studies suggested that the tick microbial communities are under the influence of several factors including the tick species, dietary bloodmeal, and physiological stress. Compared with insects, very little of the microbial community is known to contribute to the nutrition of the host. Therefore, it is of significance to elucidate the regulation of the microbial community of Rh. Sanguineus under starvation stress. Starvation stress was induced in wild-type adults (1 month, 2 months, 4 months, 6 months) and the microbial composition and diversity were analyzed before and after blood feeding. After the evaluation, it was found that the microbial community composition of Rh. sanguineus changed significantly with starvation stress. The dominant symbiotic bacteria Coxiella spp. of Rh. sanguineus gradually decreased with the prolongation of starvation stress. We also demonstrated that the starvation tolerance of Rh. sanguineus was as long as 6 months. Next, Coxiella-like endosymbionts were quantitatively analyzed by fluorescence quantitative PCR. We found a pronounced tissue tropism in the Malpighian tubule and female gonad, and less in the midgut and salivary gland organs. Finally, the blood-fed nymphs were injected with ofloxacin within 24 h. The nymphs were allowed to develop into adults. It was found that the adult blood-sucking rate, adult weight after blood meal, fecundity (egg hatching rate), and feeding period of the newly hatched larvae were all affected to varying degrees, indicating that the removal of most symbiotic bacteria had an irreversible effect on it. Frontiers Media S.A. 2022-09-09 /pmc/articles/PMC9504665/ /pubmed/36160860 http://dx.doi.org/10.3389/fphys.2022.932130 Text en Copyright © 2022 An, Bhowmick, Liang, Suo, Liao, Zhao and Han. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
An, Liping
Bhowmick, Biswajit
Liang, Dejuan
Suo, Penghui
Liao, Chenghong
Zhao, Jianguo
Han, Qian
The microbiota changes of the brown dog tick, Rhipicephalus sanguineus under starvation stress
title The microbiota changes of the brown dog tick, Rhipicephalus sanguineus under starvation stress
title_full The microbiota changes of the brown dog tick, Rhipicephalus sanguineus under starvation stress
title_fullStr The microbiota changes of the brown dog tick, Rhipicephalus sanguineus under starvation stress
title_full_unstemmed The microbiota changes of the brown dog tick, Rhipicephalus sanguineus under starvation stress
title_short The microbiota changes of the brown dog tick, Rhipicephalus sanguineus under starvation stress
title_sort microbiota changes of the brown dog tick, rhipicephalus sanguineus under starvation stress
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504665/
https://www.ncbi.nlm.nih.gov/pubmed/36160860
http://dx.doi.org/10.3389/fphys.2022.932130
work_keys_str_mv AT anliping themicrobiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT bhowmickbiswajit themicrobiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT liangdejuan themicrobiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT suopenghui themicrobiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT liaochenghong themicrobiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT zhaojianguo themicrobiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT hanqian themicrobiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT anliping microbiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT bhowmickbiswajit microbiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT liangdejuan microbiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT suopenghui microbiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT liaochenghong microbiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT zhaojianguo microbiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress
AT hanqian microbiotachangesofthebrowndogtickrhipicephalussanguineusunderstarvationstress