Cargando…
Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging
Emulgel is a new innovatory technique for drug development permitting controlled release of active ingredients for topical administration. We report a stable emulgel of 4% Piper nigrum extract (PNE) prepared using 80% ethanol. The PNE-loaded formulation had an antioxidant activity of 84% and tyrosin...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504714/ https://www.ncbi.nlm.nih.gov/pubmed/36144719 http://dx.doi.org/10.3390/molecules27185990 |
_version_ | 1784796286377000960 |
---|---|
author | Yousuf, Muhammad Khan, Haji Muhammad Shoaib Rasool, Fatima Khan, Kashif ur Rehman Usman, Faisal Ghalloo, Bilal Ahmad Umair, Muhammad Babalghith, Ahmad O. Kamran, Muhammad Aadil, Rana Muhammad Al Jaouni, Soad K. Selim, Samy Korma, Sameh A. Conte-Junior, Carlos Adam |
author_facet | Yousuf, Muhammad Khan, Haji Muhammad Shoaib Rasool, Fatima Khan, Kashif ur Rehman Usman, Faisal Ghalloo, Bilal Ahmad Umair, Muhammad Babalghith, Ahmad O. Kamran, Muhammad Aadil, Rana Muhammad Al Jaouni, Soad K. Selim, Samy Korma, Sameh A. Conte-Junior, Carlos Adam |
author_sort | Yousuf, Muhammad |
collection | PubMed |
description | Emulgel is a new innovatory technique for drug development permitting controlled release of active ingredients for topical administration. We report a stable emulgel of 4% Piper nigrum extract (PNE) prepared using 80% ethanol. The PNE-loaded formulation had an antioxidant activity of 84% and tyrosinase inhibition was 82%. Prepared formulation rendered spherical-shaped globules with high zeta potential (−45.5 mV) indicative of a stable system. Total phenolic contents were 58.01 mg GAE/g of dry extract whereas total flavonoid content was 52.63 mg QE/g of dry extract. Sun protection factor for PNE-loaded emulgel was 7.512 and formulation was stable without any evidence of physical and chemical changes following 90 days of storage. Gas chromatography-mass spectroscopy (GC-MS) revealed seventeen bioactive compounds in the PNE including monoterpenoids, triterpenoids, a tertiary alcohol, fatty acid esters, and phytosterols. In silico studies of GC-MS identified compounds show higher binding affinity in comparison to standard kojic acid indicating tyrosinase inhibition. It can be concluded that PNE-loaded emulgel had prominent antioxidant and tyrosinase inhibition and can be utilized as a promising topical system for anti-aging skin formulation. |
format | Online Article Text |
id | pubmed-9504714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95047142022-09-24 Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging Yousuf, Muhammad Khan, Haji Muhammad Shoaib Rasool, Fatima Khan, Kashif ur Rehman Usman, Faisal Ghalloo, Bilal Ahmad Umair, Muhammad Babalghith, Ahmad O. Kamran, Muhammad Aadil, Rana Muhammad Al Jaouni, Soad K. Selim, Samy Korma, Sameh A. Conte-Junior, Carlos Adam Molecules Article Emulgel is a new innovatory technique for drug development permitting controlled release of active ingredients for topical administration. We report a stable emulgel of 4% Piper nigrum extract (PNE) prepared using 80% ethanol. The PNE-loaded formulation had an antioxidant activity of 84% and tyrosinase inhibition was 82%. Prepared formulation rendered spherical-shaped globules with high zeta potential (−45.5 mV) indicative of a stable system. Total phenolic contents were 58.01 mg GAE/g of dry extract whereas total flavonoid content was 52.63 mg QE/g of dry extract. Sun protection factor for PNE-loaded emulgel was 7.512 and formulation was stable without any evidence of physical and chemical changes following 90 days of storage. Gas chromatography-mass spectroscopy (GC-MS) revealed seventeen bioactive compounds in the PNE including monoterpenoids, triterpenoids, a tertiary alcohol, fatty acid esters, and phytosterols. In silico studies of GC-MS identified compounds show higher binding affinity in comparison to standard kojic acid indicating tyrosinase inhibition. It can be concluded that PNE-loaded emulgel had prominent antioxidant and tyrosinase inhibition and can be utilized as a promising topical system for anti-aging skin formulation. MDPI 2022-09-14 /pmc/articles/PMC9504714/ /pubmed/36144719 http://dx.doi.org/10.3390/molecules27185990 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yousuf, Muhammad Khan, Haji Muhammad Shoaib Rasool, Fatima Khan, Kashif ur Rehman Usman, Faisal Ghalloo, Bilal Ahmad Umair, Muhammad Babalghith, Ahmad O. Kamran, Muhammad Aadil, Rana Muhammad Al Jaouni, Soad K. Selim, Samy Korma, Sameh A. Conte-Junior, Carlos Adam Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging |
title | Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging |
title_full | Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging |
title_fullStr | Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging |
title_full_unstemmed | Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging |
title_short | Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging |
title_sort | chemical profiling, formulation development, in vitro evaluation and molecular docking of piper nigrum seeds extract loaded emulgel for anti-aging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504714/ https://www.ncbi.nlm.nih.gov/pubmed/36144719 http://dx.doi.org/10.3390/molecules27185990 |
work_keys_str_mv | AT yousufmuhammad chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT khanhajimuhammadshoaib chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT rasoolfatima chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT khankashifurrehman chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT usmanfaisal chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT ghalloobilalahmad chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT umairmuhammad chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT babalghithahmado chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT kamranmuhammad chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT aadilranamuhammad chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT aljaounisoadk chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT selimsamy chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT kormasameha chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging AT contejuniorcarlosadam chemicalprofilingformulationdevelopmentinvitroevaluationandmoleculardockingofpipernigrumseedsextractloadedemulgelforantiaging |