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Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging

Emulgel is a new innovatory technique for drug development permitting controlled release of active ingredients for topical administration. We report a stable emulgel of 4% Piper nigrum extract (PNE) prepared using 80% ethanol. The PNE-loaded formulation had an antioxidant activity of 84% and tyrosin...

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Autores principales: Yousuf, Muhammad, Khan, Haji Muhammad Shoaib, Rasool, Fatima, Khan, Kashif ur Rehman, Usman, Faisal, Ghalloo, Bilal Ahmad, Umair, Muhammad, Babalghith, Ahmad O., Kamran, Muhammad, Aadil, Rana Muhammad, Al Jaouni, Soad K., Selim, Samy, Korma, Sameh A., Conte-Junior, Carlos Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504714/
https://www.ncbi.nlm.nih.gov/pubmed/36144719
http://dx.doi.org/10.3390/molecules27185990
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author Yousuf, Muhammad
Khan, Haji Muhammad Shoaib
Rasool, Fatima
Khan, Kashif ur Rehman
Usman, Faisal
Ghalloo, Bilal Ahmad
Umair, Muhammad
Babalghith, Ahmad O.
Kamran, Muhammad
Aadil, Rana Muhammad
Al Jaouni, Soad K.
Selim, Samy
Korma, Sameh A.
Conte-Junior, Carlos Adam
author_facet Yousuf, Muhammad
Khan, Haji Muhammad Shoaib
Rasool, Fatima
Khan, Kashif ur Rehman
Usman, Faisal
Ghalloo, Bilal Ahmad
Umair, Muhammad
Babalghith, Ahmad O.
Kamran, Muhammad
Aadil, Rana Muhammad
Al Jaouni, Soad K.
Selim, Samy
Korma, Sameh A.
Conte-Junior, Carlos Adam
author_sort Yousuf, Muhammad
collection PubMed
description Emulgel is a new innovatory technique for drug development permitting controlled release of active ingredients for topical administration. We report a stable emulgel of 4% Piper nigrum extract (PNE) prepared using 80% ethanol. The PNE-loaded formulation had an antioxidant activity of 84% and tyrosinase inhibition was 82%. Prepared formulation rendered spherical-shaped globules with high zeta potential (−45.5 mV) indicative of a stable system. Total phenolic contents were 58.01 mg GAE/g of dry extract whereas total flavonoid content was 52.63 mg QE/g of dry extract. Sun protection factor for PNE-loaded emulgel was 7.512 and formulation was stable without any evidence of physical and chemical changes following 90 days of storage. Gas chromatography-mass spectroscopy (GC-MS) revealed seventeen bioactive compounds in the PNE including monoterpenoids, triterpenoids, a tertiary alcohol, fatty acid esters, and phytosterols. In silico studies of GC-MS identified compounds show higher binding affinity in comparison to standard kojic acid indicating tyrosinase inhibition. It can be concluded that PNE-loaded emulgel had prominent antioxidant and tyrosinase inhibition and can be utilized as a promising topical system for anti-aging skin formulation.
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spelling pubmed-95047142022-09-24 Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging Yousuf, Muhammad Khan, Haji Muhammad Shoaib Rasool, Fatima Khan, Kashif ur Rehman Usman, Faisal Ghalloo, Bilal Ahmad Umair, Muhammad Babalghith, Ahmad O. Kamran, Muhammad Aadil, Rana Muhammad Al Jaouni, Soad K. Selim, Samy Korma, Sameh A. Conte-Junior, Carlos Adam Molecules Article Emulgel is a new innovatory technique for drug development permitting controlled release of active ingredients for topical administration. We report a stable emulgel of 4% Piper nigrum extract (PNE) prepared using 80% ethanol. The PNE-loaded formulation had an antioxidant activity of 84% and tyrosinase inhibition was 82%. Prepared formulation rendered spherical-shaped globules with high zeta potential (−45.5 mV) indicative of a stable system. Total phenolic contents were 58.01 mg GAE/g of dry extract whereas total flavonoid content was 52.63 mg QE/g of dry extract. Sun protection factor for PNE-loaded emulgel was 7.512 and formulation was stable without any evidence of physical and chemical changes following 90 days of storage. Gas chromatography-mass spectroscopy (GC-MS) revealed seventeen bioactive compounds in the PNE including monoterpenoids, triterpenoids, a tertiary alcohol, fatty acid esters, and phytosterols. In silico studies of GC-MS identified compounds show higher binding affinity in comparison to standard kojic acid indicating tyrosinase inhibition. It can be concluded that PNE-loaded emulgel had prominent antioxidant and tyrosinase inhibition and can be utilized as a promising topical system for anti-aging skin formulation. MDPI 2022-09-14 /pmc/articles/PMC9504714/ /pubmed/36144719 http://dx.doi.org/10.3390/molecules27185990 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yousuf, Muhammad
Khan, Haji Muhammad Shoaib
Rasool, Fatima
Khan, Kashif ur Rehman
Usman, Faisal
Ghalloo, Bilal Ahmad
Umair, Muhammad
Babalghith, Ahmad O.
Kamran, Muhammad
Aadil, Rana Muhammad
Al Jaouni, Soad K.
Selim, Samy
Korma, Sameh A.
Conte-Junior, Carlos Adam
Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging
title Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging
title_full Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging
title_fullStr Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging
title_full_unstemmed Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging
title_short Chemical Profiling, Formulation Development, In Vitro Evaluation and Molecular Docking of Piper nigrum Seeds Extract Loaded Emulgel for Anti-Aging
title_sort chemical profiling, formulation development, in vitro evaluation and molecular docking of piper nigrum seeds extract loaded emulgel for anti-aging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504714/
https://www.ncbi.nlm.nih.gov/pubmed/36144719
http://dx.doi.org/10.3390/molecules27185990
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