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Effective Approaches to Study the Genetic Variability of SARS-CoV-2

Significant efforts are being made in many countries around the world to respond to the COVID-19 pandemic by developing diagnostic reagent kits, identifying infected people, determining treatment methods, and finally producing effective vaccines. However, novel coronavirus variants may potentially r...

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Autores principales: Kotov, Ivan, Saenko, Valeriia, Borisova, Nadezhda, Kolesnikov, Anton, Kondrasheva, Larisa, Tivanova, Elena, Khafizov, Kamil, Akimkin, Vasily
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504788/
https://www.ncbi.nlm.nih.gov/pubmed/36146662
http://dx.doi.org/10.3390/v14091855
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author Kotov, Ivan
Saenko, Valeriia
Borisova, Nadezhda
Kolesnikov, Anton
Kondrasheva, Larisa
Tivanova, Elena
Khafizov, Kamil
Akimkin, Vasily
author_facet Kotov, Ivan
Saenko, Valeriia
Borisova, Nadezhda
Kolesnikov, Anton
Kondrasheva, Larisa
Tivanova, Elena
Khafizov, Kamil
Akimkin, Vasily
author_sort Kotov, Ivan
collection PubMed
description Significant efforts are being made in many countries around the world to respond to the COVID-19 pandemic by developing diagnostic reagent kits, identifying infected people, determining treatment methods, and finally producing effective vaccines. However, novel coronavirus variants may potentially reduce the effectiveness of all these efforts, demonstrating increased transmissibility and abated response to therapy or vaccines, as well as the possibility of false negative results in diagnostic procedures based on nucleic acid amplification methods. Since the end of 2020, several variants of concern have been discovered around the world. When information about a new, potentially more dangerous strain of pathogen appears, it is crucial to determine the moment of its emergence in a region. Eventually, that permits taking timely measures and minimizing new risks associated with the spreading of the virus. Therefore, numerous nations have made tremendous efforts to identify and trace these virus variants, which necessitates serious technological processes to sequence a large number of viral genomes. Here, we report on our experience as one of the primary laboratories involved in monitoring SARS-CoV-2 variants in Russia. We discuss the various approaches used, describe effective protocols, and outline a potential technique combining several methods to increase the ability to trace genetic variants while minimizing financial and labor costs.
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spelling pubmed-95047882022-09-24 Effective Approaches to Study the Genetic Variability of SARS-CoV-2 Kotov, Ivan Saenko, Valeriia Borisova, Nadezhda Kolesnikov, Anton Kondrasheva, Larisa Tivanova, Elena Khafizov, Kamil Akimkin, Vasily Viruses Article Significant efforts are being made in many countries around the world to respond to the COVID-19 pandemic by developing diagnostic reagent kits, identifying infected people, determining treatment methods, and finally producing effective vaccines. However, novel coronavirus variants may potentially reduce the effectiveness of all these efforts, demonstrating increased transmissibility and abated response to therapy or vaccines, as well as the possibility of false negative results in diagnostic procedures based on nucleic acid amplification methods. Since the end of 2020, several variants of concern have been discovered around the world. When information about a new, potentially more dangerous strain of pathogen appears, it is crucial to determine the moment of its emergence in a region. Eventually, that permits taking timely measures and minimizing new risks associated with the spreading of the virus. Therefore, numerous nations have made tremendous efforts to identify and trace these virus variants, which necessitates serious technological processes to sequence a large number of viral genomes. Here, we report on our experience as one of the primary laboratories involved in monitoring SARS-CoV-2 variants in Russia. We discuss the various approaches used, describe effective protocols, and outline a potential technique combining several methods to increase the ability to trace genetic variants while minimizing financial and labor costs. MDPI 2022-08-24 /pmc/articles/PMC9504788/ /pubmed/36146662 http://dx.doi.org/10.3390/v14091855 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kotov, Ivan
Saenko, Valeriia
Borisova, Nadezhda
Kolesnikov, Anton
Kondrasheva, Larisa
Tivanova, Elena
Khafizov, Kamil
Akimkin, Vasily
Effective Approaches to Study the Genetic Variability of SARS-CoV-2
title Effective Approaches to Study the Genetic Variability of SARS-CoV-2
title_full Effective Approaches to Study the Genetic Variability of SARS-CoV-2
title_fullStr Effective Approaches to Study the Genetic Variability of SARS-CoV-2
title_full_unstemmed Effective Approaches to Study the Genetic Variability of SARS-CoV-2
title_short Effective Approaches to Study the Genetic Variability of SARS-CoV-2
title_sort effective approaches to study the genetic variability of sars-cov-2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504788/
https://www.ncbi.nlm.nih.gov/pubmed/36146662
http://dx.doi.org/10.3390/v14091855
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