Cargando…

Mucosal Healing in Celiac Disease: Villous Architecture and Immunohistochemical Features in Children on a Long-Term Gluten Free Diet

Considerable heterogeneity exists across studies assessing intestinal mucosal recovery in celiac (CD) patients on a gluten-free diet (GFD). We aimed at investigating histological and immunohistochemical features in CD patients on a long-term GFD and to correlate them to the GFD duration. Morphometri...

Descripción completa

Detalles Bibliográficos
Autores principales: Mandile, Roberta, Maglio, Mariantonia, Mosca, Caterina, Marano, Antonella, Discepolo, Valentina, Troncone, Riccardo, Auricchio, Renata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504881/
https://www.ncbi.nlm.nih.gov/pubmed/36145072
http://dx.doi.org/10.3390/nu14183696
_version_ 1784796328743665664
author Mandile, Roberta
Maglio, Mariantonia
Mosca, Caterina
Marano, Antonella
Discepolo, Valentina
Troncone, Riccardo
Auricchio, Renata
author_facet Mandile, Roberta
Maglio, Mariantonia
Mosca, Caterina
Marano, Antonella
Discepolo, Valentina
Troncone, Riccardo
Auricchio, Renata
author_sort Mandile, Roberta
collection PubMed
description Considerable heterogeneity exists across studies assessing intestinal mucosal recovery in celiac (CD) patients on a gluten-free diet (GFD). We aimed at investigating histological and immunohistochemical features in CD patients on a long-term GFD and to correlate them to the GFD duration. Morphometrical and immunohistochemical analysis were retrospectively performed on duodenal biopsies in three groups of children: 33 on a long-term (>2 years) GFD (GFD-group), four of which remained seropositive despite dietary adherence, 31 with villous atrophy (ACD-group) and 76 heathy, non-celiac (CTR-group). Moreover, in the GFD-group, we correlated immunohistochemical alterations to the GFD duration. The villous to crypt (V/C) ratio significantly improved after the GFD and completely normalized in all patients, becoming even higher than in the CTR-group (median value 3.2 vs. 3, p = 0.007). In parallel, the number of CD3+ and TCRγδ+ cells in the epithelium were significantly reduced in the GFD compared to ACD patients, even if they remained higher than in the CTR-group (p < 0.05). In contrast, CD25+ cells in the lamina propria significantly decreased after the GFD (p < 0.05) and become comparable to the CTR-group (p = 0.9). In the GFD-group there was no difference in the immunohistochemical parameters between seropositive and seronegative patients and alterations did not correlate to GFD length. In conclusion, a GFD is able to both restore a normal V/C ratio and reduce inflammation, but the epithelium maintains some stigmata of the disorder, such as an increased number of CD3+ and TCRγδ+ cells. These alterations persist regardless of the duration of the GFD.
format Online
Article
Text
id pubmed-9504881
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95048812022-09-24 Mucosal Healing in Celiac Disease: Villous Architecture and Immunohistochemical Features in Children on a Long-Term Gluten Free Diet Mandile, Roberta Maglio, Mariantonia Mosca, Caterina Marano, Antonella Discepolo, Valentina Troncone, Riccardo Auricchio, Renata Nutrients Article Considerable heterogeneity exists across studies assessing intestinal mucosal recovery in celiac (CD) patients on a gluten-free diet (GFD). We aimed at investigating histological and immunohistochemical features in CD patients on a long-term GFD and to correlate them to the GFD duration. Morphometrical and immunohistochemical analysis were retrospectively performed on duodenal biopsies in three groups of children: 33 on a long-term (>2 years) GFD (GFD-group), four of which remained seropositive despite dietary adherence, 31 with villous atrophy (ACD-group) and 76 heathy, non-celiac (CTR-group). Moreover, in the GFD-group, we correlated immunohistochemical alterations to the GFD duration. The villous to crypt (V/C) ratio significantly improved after the GFD and completely normalized in all patients, becoming even higher than in the CTR-group (median value 3.2 vs. 3, p = 0.007). In parallel, the number of CD3+ and TCRγδ+ cells in the epithelium were significantly reduced in the GFD compared to ACD patients, even if they remained higher than in the CTR-group (p < 0.05). In contrast, CD25+ cells in the lamina propria significantly decreased after the GFD (p < 0.05) and become comparable to the CTR-group (p = 0.9). In the GFD-group there was no difference in the immunohistochemical parameters between seropositive and seronegative patients and alterations did not correlate to GFD length. In conclusion, a GFD is able to both restore a normal V/C ratio and reduce inflammation, but the epithelium maintains some stigmata of the disorder, such as an increased number of CD3+ and TCRγδ+ cells. These alterations persist regardless of the duration of the GFD. MDPI 2022-09-07 /pmc/articles/PMC9504881/ /pubmed/36145072 http://dx.doi.org/10.3390/nu14183696 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mandile, Roberta
Maglio, Mariantonia
Mosca, Caterina
Marano, Antonella
Discepolo, Valentina
Troncone, Riccardo
Auricchio, Renata
Mucosal Healing in Celiac Disease: Villous Architecture and Immunohistochemical Features in Children on a Long-Term Gluten Free Diet
title Mucosal Healing in Celiac Disease: Villous Architecture and Immunohistochemical Features in Children on a Long-Term Gluten Free Diet
title_full Mucosal Healing in Celiac Disease: Villous Architecture and Immunohistochemical Features in Children on a Long-Term Gluten Free Diet
title_fullStr Mucosal Healing in Celiac Disease: Villous Architecture and Immunohistochemical Features in Children on a Long-Term Gluten Free Diet
title_full_unstemmed Mucosal Healing in Celiac Disease: Villous Architecture and Immunohistochemical Features in Children on a Long-Term Gluten Free Diet
title_short Mucosal Healing in Celiac Disease: Villous Architecture and Immunohistochemical Features in Children on a Long-Term Gluten Free Diet
title_sort mucosal healing in celiac disease: villous architecture and immunohistochemical features in children on a long-term gluten free diet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504881/
https://www.ncbi.nlm.nih.gov/pubmed/36145072
http://dx.doi.org/10.3390/nu14183696
work_keys_str_mv AT mandileroberta mucosalhealinginceliacdiseasevillousarchitectureandimmunohistochemicalfeaturesinchildrenonalongtermglutenfreediet
AT magliomariantonia mucosalhealinginceliacdiseasevillousarchitectureandimmunohistochemicalfeaturesinchildrenonalongtermglutenfreediet
AT moscacaterina mucosalhealinginceliacdiseasevillousarchitectureandimmunohistochemicalfeaturesinchildrenonalongtermglutenfreediet
AT maranoantonella mucosalhealinginceliacdiseasevillousarchitectureandimmunohistochemicalfeaturesinchildrenonalongtermglutenfreediet
AT discepolovalentina mucosalhealinginceliacdiseasevillousarchitectureandimmunohistochemicalfeaturesinchildrenonalongtermglutenfreediet
AT tronconericcardo mucosalhealinginceliacdiseasevillousarchitectureandimmunohistochemicalfeaturesinchildrenonalongtermglutenfreediet
AT auricchiorenata mucosalhealinginceliacdiseasevillousarchitectureandimmunohistochemicalfeaturesinchildrenonalongtermglutenfreediet