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Fusarium spp. Associated with Dendrobium officinale Dieback Disease in China

A rare plant species of the Orchidaceae family, Dendrobium officinale is considered among the top ten Chinese medicinal herbs for its polysaccharide. Since 2021, when the dieback disease of D. officinale was first reported in Yueqing City, Zhejiang Province, China, Fusarium isolates (number = 152) w...

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Autores principales: Mirghasempour, Seyed Ali, Michailides, Themis, Chen, Weiliang, Mao, Bizeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504887/
https://www.ncbi.nlm.nih.gov/pubmed/36135644
http://dx.doi.org/10.3390/jof8090919
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author Mirghasempour, Seyed Ali
Michailides, Themis
Chen, Weiliang
Mao, Bizeng
author_facet Mirghasempour, Seyed Ali
Michailides, Themis
Chen, Weiliang
Mao, Bizeng
author_sort Mirghasempour, Seyed Ali
collection PubMed
description A rare plant species of the Orchidaceae family, Dendrobium officinale is considered among the top ten Chinese medicinal herbs for its polysaccharide. Since 2021, when the dieback disease of D. officinale was first reported in Yueqing City, Zhejiang Province, China, Fusarium isolates (number = 152) were obtained from 70 plants in commercial greenhouses. The disease incidence ranged from 40% to 60% in the surveyed areas. Multilocus sequence analysis (MLSA) coupled with morphological characterization revealed that the collected isolates belonged to five species (sp.), viz., Fusarium concentricum, F. fujikuroi, F. nirenbergiae, F. curvatum, and F. stilboides, with isolation frequencies of 34.6%, 22.3%, 18.4%, 13.8%, and 10.5%, respectively. Notably, at least two Fusarium species were simultaneously isolated and identified from the infected plants. Finally, the pathogenicity test results demonstrated that such species were responsible for the dieback disease of D. officinale. However, F. concentricum and F. fujikuroi were more invasive compared to the other species in this study. To the best of the authors’ knowledge, this study was the first report of F. concentricum, F. curvatum, F. fujikuroi, F. nirenbergiae, and F. stilboides causing the dieback disease of D. officinale in China and worldwide. This work provides valuable data about the diversity and pathogenicity of Fusarium populations, which will help in formulating effective strategies and policies for better control of the dieback disease.
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spelling pubmed-95048872022-09-24 Fusarium spp. Associated with Dendrobium officinale Dieback Disease in China Mirghasempour, Seyed Ali Michailides, Themis Chen, Weiliang Mao, Bizeng J Fungi (Basel) Article A rare plant species of the Orchidaceae family, Dendrobium officinale is considered among the top ten Chinese medicinal herbs for its polysaccharide. Since 2021, when the dieback disease of D. officinale was first reported in Yueqing City, Zhejiang Province, China, Fusarium isolates (number = 152) were obtained from 70 plants in commercial greenhouses. The disease incidence ranged from 40% to 60% in the surveyed areas. Multilocus sequence analysis (MLSA) coupled with morphological characterization revealed that the collected isolates belonged to five species (sp.), viz., Fusarium concentricum, F. fujikuroi, F. nirenbergiae, F. curvatum, and F. stilboides, with isolation frequencies of 34.6%, 22.3%, 18.4%, 13.8%, and 10.5%, respectively. Notably, at least two Fusarium species were simultaneously isolated and identified from the infected plants. Finally, the pathogenicity test results demonstrated that such species were responsible for the dieback disease of D. officinale. However, F. concentricum and F. fujikuroi were more invasive compared to the other species in this study. To the best of the authors’ knowledge, this study was the first report of F. concentricum, F. curvatum, F. fujikuroi, F. nirenbergiae, and F. stilboides causing the dieback disease of D. officinale in China and worldwide. This work provides valuable data about the diversity and pathogenicity of Fusarium populations, which will help in formulating effective strategies and policies for better control of the dieback disease. MDPI 2022-08-29 /pmc/articles/PMC9504887/ /pubmed/36135644 http://dx.doi.org/10.3390/jof8090919 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mirghasempour, Seyed Ali
Michailides, Themis
Chen, Weiliang
Mao, Bizeng
Fusarium spp. Associated with Dendrobium officinale Dieback Disease in China
title Fusarium spp. Associated with Dendrobium officinale Dieback Disease in China
title_full Fusarium spp. Associated with Dendrobium officinale Dieback Disease in China
title_fullStr Fusarium spp. Associated with Dendrobium officinale Dieback Disease in China
title_full_unstemmed Fusarium spp. Associated with Dendrobium officinale Dieback Disease in China
title_short Fusarium spp. Associated with Dendrobium officinale Dieback Disease in China
title_sort fusarium spp. associated with dendrobium officinale dieback disease in china
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504887/
https://www.ncbi.nlm.nih.gov/pubmed/36135644
http://dx.doi.org/10.3390/jof8090919
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