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Diverse Genotypes of Cryptosporidium in Sheep in California, USA

Cryptosporidium spp. is a parasite that can infect a wide variety of vertebrate species. The parasite has been detected in sheep worldwide with diverse species and genotypes of various levels of zoonotic potential and public health concern. The purpose of this study was to determine the distribution...

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Autores principales: Li, Xunde, Vodovoza, Tamara, Atwill, Edward R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504958/
https://www.ncbi.nlm.nih.gov/pubmed/36145455
http://dx.doi.org/10.3390/pathogens11091023
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author Li, Xunde
Vodovoza, Tamara
Atwill, Edward R.
author_facet Li, Xunde
Vodovoza, Tamara
Atwill, Edward R.
author_sort Li, Xunde
collection PubMed
description Cryptosporidium spp. is a parasite that can infect a wide variety of vertebrate species. The parasite has been detected in sheep worldwide with diverse species and genotypes of various levels of zoonotic potential and public health concern. The purpose of this study was to determine the distribution of genotypes of Cryptosporidium in sheep in California, USA. Microscopic positive samples from individual sheep from central and northern California ranches were genotyped by sequencing a fragment of the 18S rRNA gene and BLAST analysis. Eighty-eight (63.8%) of the microscopic positive samples were genotyped, and multiple genotypes of Cryptosporidium were identified from sheep in the enrolled ranches. Approximately 89% of isolates (n = 78) were C. xiaoi or C. bovis, 10% of isolates (n = 9) were C. ubiquitum, and 1% of isolates (n = 1) were C. parvum. The C. parvum and C. ubiquitum isolates were detected only from lambs and limited to four farms. Given that the majority of Cryptosporidium species (i.e., C. xiaoi and C. bovis) were of minor zoonotic concern, the results of this study suggest that sheep are not a reservoir of major zoonotic Cryptosporidium in California ranches.
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spelling pubmed-95049582022-09-24 Diverse Genotypes of Cryptosporidium in Sheep in California, USA Li, Xunde Vodovoza, Tamara Atwill, Edward R. Pathogens Article Cryptosporidium spp. is a parasite that can infect a wide variety of vertebrate species. The parasite has been detected in sheep worldwide with diverse species and genotypes of various levels of zoonotic potential and public health concern. The purpose of this study was to determine the distribution of genotypes of Cryptosporidium in sheep in California, USA. Microscopic positive samples from individual sheep from central and northern California ranches were genotyped by sequencing a fragment of the 18S rRNA gene and BLAST analysis. Eighty-eight (63.8%) of the microscopic positive samples were genotyped, and multiple genotypes of Cryptosporidium were identified from sheep in the enrolled ranches. Approximately 89% of isolates (n = 78) were C. xiaoi or C. bovis, 10% of isolates (n = 9) were C. ubiquitum, and 1% of isolates (n = 1) were C. parvum. The C. parvum and C. ubiquitum isolates were detected only from lambs and limited to four farms. Given that the majority of Cryptosporidium species (i.e., C. xiaoi and C. bovis) were of minor zoonotic concern, the results of this study suggest that sheep are not a reservoir of major zoonotic Cryptosporidium in California ranches. MDPI 2022-09-08 /pmc/articles/PMC9504958/ /pubmed/36145455 http://dx.doi.org/10.3390/pathogens11091023 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Xunde
Vodovoza, Tamara
Atwill, Edward R.
Diverse Genotypes of Cryptosporidium in Sheep in California, USA
title Diverse Genotypes of Cryptosporidium in Sheep in California, USA
title_full Diverse Genotypes of Cryptosporidium in Sheep in California, USA
title_fullStr Diverse Genotypes of Cryptosporidium in Sheep in California, USA
title_full_unstemmed Diverse Genotypes of Cryptosporidium in Sheep in California, USA
title_short Diverse Genotypes of Cryptosporidium in Sheep in California, USA
title_sort diverse genotypes of cryptosporidium in sheep in california, usa
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504958/
https://www.ncbi.nlm.nih.gov/pubmed/36145455
http://dx.doi.org/10.3390/pathogens11091023
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