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Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids in Chlamydomonas reinhardtii

Eukaryotic green microalgae show considerable promise for the sustainable light-driven biosynthesis of high-value fine chemicals, especially terpenoids because of their fast and inexpensive phototrophic growth. Here, the novel isopentenol utilization pathway (IUP) was introduced into Chlamydomonas r...

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Autores principales: Zhao, Mei-Li, Cai, Wen-Sheng, Zheng, Si-Qi, Zhao, Jia-Lin, Zhang, Jun-Liang, Huang, Ying, Hu, Zhang-Li, Jia, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505001/
https://www.ncbi.nlm.nih.gov/pubmed/36135766
http://dx.doi.org/10.3390/md20090577
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author Zhao, Mei-Li
Cai, Wen-Sheng
Zheng, Si-Qi
Zhao, Jia-Lin
Zhang, Jun-Liang
Huang, Ying
Hu, Zhang-Li
Jia, Bin
author_facet Zhao, Mei-Li
Cai, Wen-Sheng
Zheng, Si-Qi
Zhao, Jia-Lin
Zhang, Jun-Liang
Huang, Ying
Hu, Zhang-Li
Jia, Bin
author_sort Zhao, Mei-Li
collection PubMed
description Eukaryotic green microalgae show considerable promise for the sustainable light-driven biosynthesis of high-value fine chemicals, especially terpenoids because of their fast and inexpensive phototrophic growth. Here, the novel isopentenol utilization pathway (IUP) was introduced into Chlamydomonas reinhardtii to enhance the hemiterpene (isopentenyl pyrophosphate, IPP) titers. Then, diphosphate isomerase (IDI) and limonene synthase (MsLS) were further inserted for limonene production. Transgenic algae showed 8.6-fold increase in IPP compared with the wild type, and 23-fold increase in limonene production compared with a single MsLS expressing strain. Following the culture optimization, the highest limonene production reached 117 µg/L, when the strain was cultured in a opt2 medium supplemented with 10 mM isoprenol under a light: dark regimen. This demonstrates that transgenic algae expressing the IUP represent an ideal chassis for the high-value terpenoid production. The IUP will facilitate further the metabolic and enzyme engineering to enhance the terpenoid titers by significantly reducing the number of enzyme steps required for an optimal biosynthesis.
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spelling pubmed-95050012022-09-24 Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids in Chlamydomonas reinhardtii Zhao, Mei-Li Cai, Wen-Sheng Zheng, Si-Qi Zhao, Jia-Lin Zhang, Jun-Liang Huang, Ying Hu, Zhang-Li Jia, Bin Mar Drugs Article Eukaryotic green microalgae show considerable promise for the sustainable light-driven biosynthesis of high-value fine chemicals, especially terpenoids because of their fast and inexpensive phototrophic growth. Here, the novel isopentenol utilization pathway (IUP) was introduced into Chlamydomonas reinhardtii to enhance the hemiterpene (isopentenyl pyrophosphate, IPP) titers. Then, diphosphate isomerase (IDI) and limonene synthase (MsLS) were further inserted for limonene production. Transgenic algae showed 8.6-fold increase in IPP compared with the wild type, and 23-fold increase in limonene production compared with a single MsLS expressing strain. Following the culture optimization, the highest limonene production reached 117 µg/L, when the strain was cultured in a opt2 medium supplemented with 10 mM isoprenol under a light: dark regimen. This demonstrates that transgenic algae expressing the IUP represent an ideal chassis for the high-value terpenoid production. The IUP will facilitate further the metabolic and enzyme engineering to enhance the terpenoid titers by significantly reducing the number of enzyme steps required for an optimal biosynthesis. MDPI 2022-09-15 /pmc/articles/PMC9505001/ /pubmed/36135766 http://dx.doi.org/10.3390/md20090577 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Mei-Li
Cai, Wen-Sheng
Zheng, Si-Qi
Zhao, Jia-Lin
Zhang, Jun-Liang
Huang, Ying
Hu, Zhang-Li
Jia, Bin
Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids in Chlamydomonas reinhardtii
title Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids in Chlamydomonas reinhardtii
title_full Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids in Chlamydomonas reinhardtii
title_fullStr Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids in Chlamydomonas reinhardtii
title_full_unstemmed Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids in Chlamydomonas reinhardtii
title_short Metabolic Engineering of the Isopentenol Utilization Pathway Enhanced the Production of Terpenoids in Chlamydomonas reinhardtii
title_sort metabolic engineering of the isopentenol utilization pathway enhanced the production of terpenoids in chlamydomonas reinhardtii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505001/
https://www.ncbi.nlm.nih.gov/pubmed/36135766
http://dx.doi.org/10.3390/md20090577
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