Cargando…

Genome-Wide Identification and Characterization of Heat Shock Protein 20 Genes in Maize

Maize is an important cereal crop worldwide and is sensitive to abiotic stresses in fluctuant environments that seriously affect its growth, yield, and quality. The small heat shock protein (HSP20) plays a crucial role in protecting plants from abiotic stress. However, little is known about HSP20 in...

Descripción completa

Detalles Bibliográficos
Autores principales: Qi, Huanhuan, Chen, Xiaoke, Luo, Sen, Fan, Hongzeng, Guo, Jinghua, Zhang, Xuehai, Ke, Yinggen, Yang, Pingfang, Yu, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505046/
https://www.ncbi.nlm.nih.gov/pubmed/36143433
http://dx.doi.org/10.3390/life12091397
_version_ 1784796374676537344
author Qi, Huanhuan
Chen, Xiaoke
Luo, Sen
Fan, Hongzeng
Guo, Jinghua
Zhang, Xuehai
Ke, Yinggen
Yang, Pingfang
Yu, Feng
author_facet Qi, Huanhuan
Chen, Xiaoke
Luo, Sen
Fan, Hongzeng
Guo, Jinghua
Zhang, Xuehai
Ke, Yinggen
Yang, Pingfang
Yu, Feng
author_sort Qi, Huanhuan
collection PubMed
description Maize is an important cereal crop worldwide and is sensitive to abiotic stresses in fluctuant environments that seriously affect its growth, yield, and quality. The small heat shock protein (HSP20) plays a crucial role in protecting plants from abiotic stress. However, little is known about HSP20 in maize (ZmHSP20). In this study, 44 ZmHSP20s were identified, which were unequally distributed over 10 chromosomes, and 6 pairs of ZmHSP20s were tandemly presented. The gene structure of ZmHSP20s was highly conserved, with 95% (42) of the genes having no more than one intron. The analysis of the cis-element in ZmHSP20s promoter demonstrated large amounts of elements related to hormonal and abiotic stress responses, including abscisic acid (ABA), high temperature, and hypoxia. The ZmHSP20s protein had more than two conserved motifs that were predictably localized in the cytoplasm, nucleus, endoplasmic reticulum, peroxisome, mitochondria, and plasma. Phylogenetic analysis using HSP20s in Arabidopsis, rice, maize, and Solanum tuberosum indicated that ZmHSP20s were classified into 11 categories, of which each category had unique subcellular localization. Approximately 80% (35) of ZmHSP20 were upregulated under heat stress at the maize seedling stage, whereas the opposite expression profiling of 10 genes under 37 and 48 °C was detected. A total of 20 genes were randomly selected to investigate their expression under treatments of ABA, gibberellin (GA), ethylene, low temperature, drought, and waterlogging, and the results displayed that more than half of these genes were downregulated while ZmHSP20-3, ZmHSP20-7, ZmHSP20-24, and ZmHSP20-44 were upregulated under 1 h treatment of ethylene. A yeast-one-hybrid experiment was conducted to analyze the binding of four heat stress transcription factors (ZmHSFs) with eight of the ZmHSP20s promoter sequences, in which ZmHSF3, ZmHSF13, and ZmHSF17 can bind to most of these selected ZmHSP20s promoters. Our results provided a valuable resource for studying HSP20s function and offering candidates for genetic improvement under abiotic stress.
format Online
Article
Text
id pubmed-9505046
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95050462022-09-24 Genome-Wide Identification and Characterization of Heat Shock Protein 20 Genes in Maize Qi, Huanhuan Chen, Xiaoke Luo, Sen Fan, Hongzeng Guo, Jinghua Zhang, Xuehai Ke, Yinggen Yang, Pingfang Yu, Feng Life (Basel) Article Maize is an important cereal crop worldwide and is sensitive to abiotic stresses in fluctuant environments that seriously affect its growth, yield, and quality. The small heat shock protein (HSP20) plays a crucial role in protecting plants from abiotic stress. However, little is known about HSP20 in maize (ZmHSP20). In this study, 44 ZmHSP20s were identified, which were unequally distributed over 10 chromosomes, and 6 pairs of ZmHSP20s were tandemly presented. The gene structure of ZmHSP20s was highly conserved, with 95% (42) of the genes having no more than one intron. The analysis of the cis-element in ZmHSP20s promoter demonstrated large amounts of elements related to hormonal and abiotic stress responses, including abscisic acid (ABA), high temperature, and hypoxia. The ZmHSP20s protein had more than two conserved motifs that were predictably localized in the cytoplasm, nucleus, endoplasmic reticulum, peroxisome, mitochondria, and plasma. Phylogenetic analysis using HSP20s in Arabidopsis, rice, maize, and Solanum tuberosum indicated that ZmHSP20s were classified into 11 categories, of which each category had unique subcellular localization. Approximately 80% (35) of ZmHSP20 were upregulated under heat stress at the maize seedling stage, whereas the opposite expression profiling of 10 genes under 37 and 48 °C was detected. A total of 20 genes were randomly selected to investigate their expression under treatments of ABA, gibberellin (GA), ethylene, low temperature, drought, and waterlogging, and the results displayed that more than half of these genes were downregulated while ZmHSP20-3, ZmHSP20-7, ZmHSP20-24, and ZmHSP20-44 were upregulated under 1 h treatment of ethylene. A yeast-one-hybrid experiment was conducted to analyze the binding of four heat stress transcription factors (ZmHSFs) with eight of the ZmHSP20s promoter sequences, in which ZmHSF3, ZmHSF13, and ZmHSF17 can bind to most of these selected ZmHSP20s promoters. Our results provided a valuable resource for studying HSP20s function and offering candidates for genetic improvement under abiotic stress. MDPI 2022-09-08 /pmc/articles/PMC9505046/ /pubmed/36143433 http://dx.doi.org/10.3390/life12091397 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qi, Huanhuan
Chen, Xiaoke
Luo, Sen
Fan, Hongzeng
Guo, Jinghua
Zhang, Xuehai
Ke, Yinggen
Yang, Pingfang
Yu, Feng
Genome-Wide Identification and Characterization of Heat Shock Protein 20 Genes in Maize
title Genome-Wide Identification and Characterization of Heat Shock Protein 20 Genes in Maize
title_full Genome-Wide Identification and Characterization of Heat Shock Protein 20 Genes in Maize
title_fullStr Genome-Wide Identification and Characterization of Heat Shock Protein 20 Genes in Maize
title_full_unstemmed Genome-Wide Identification and Characterization of Heat Shock Protein 20 Genes in Maize
title_short Genome-Wide Identification and Characterization of Heat Shock Protein 20 Genes in Maize
title_sort genome-wide identification and characterization of heat shock protein 20 genes in maize
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505046/
https://www.ncbi.nlm.nih.gov/pubmed/36143433
http://dx.doi.org/10.3390/life12091397
work_keys_str_mv AT qihuanhuan genomewideidentificationandcharacterizationofheatshockprotein20genesinmaize
AT chenxiaoke genomewideidentificationandcharacterizationofheatshockprotein20genesinmaize
AT luosen genomewideidentificationandcharacterizationofheatshockprotein20genesinmaize
AT fanhongzeng genomewideidentificationandcharacterizationofheatshockprotein20genesinmaize
AT guojinghua genomewideidentificationandcharacterizationofheatshockprotein20genesinmaize
AT zhangxuehai genomewideidentificationandcharacterizationofheatshockprotein20genesinmaize
AT keyinggen genomewideidentificationandcharacterizationofheatshockprotein20genesinmaize
AT yangpingfang genomewideidentificationandcharacterizationofheatshockprotein20genesinmaize
AT yufeng genomewideidentificationandcharacterizationofheatshockprotein20genesinmaize