Cargando…

Comparison of Gene Expression Changes in Three Wheat Varieties with Different Susceptibilities to Heat Stress Using RNA-Seq Analysis

Wheat is highly susceptible to heat stress, which significantly reduces grain yield. In this study, we used RNA-seq technology to analyze the transcript expression at three different time-points after heat treatment in three cultivars differing in their susceptibility to heat stress: Jopum, Keumkang...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Myoung Hui, Kim, Kyeong-Min, Sang, Wan-Gyu, Kang, Chon-Sik, Choi, Changhyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505106/
https://www.ncbi.nlm.nih.gov/pubmed/36142649
http://dx.doi.org/10.3390/ijms231810734
_version_ 1784796389598822400
author Lee, Myoung Hui
Kim, Kyeong-Min
Sang, Wan-Gyu
Kang, Chon-Sik
Choi, Changhyun
author_facet Lee, Myoung Hui
Kim, Kyeong-Min
Sang, Wan-Gyu
Kang, Chon-Sik
Choi, Changhyun
author_sort Lee, Myoung Hui
collection PubMed
description Wheat is highly susceptible to heat stress, which significantly reduces grain yield. In this study, we used RNA-seq technology to analyze the transcript expression at three different time-points after heat treatment in three cultivars differing in their susceptibility to heat stress: Jopum, Keumkang, and Olgeuru. A total of 11,751, 8850, and 14,711; 10,959, 7946, and 14,205; and 22,895, 13,060, and 19,408 differentially-expressed genes (log2 fold-change > 1 and FDR (padj) < 0.05) were identified in Jopum, Keumkang, and Olgeuru in the control vs. 6-h, in the control vs. 12-h, and in the 6-h vs. 12-h heat treatment, respectively. Functional enrichment analysis showed that the biological processes for DEGs, such as the cellular response to heat and oxidative stress—and including the removal of superoxide radicals and the positive regulation of superoxide dismutase activity—were significantly enriched among the three comparisons in all three cultivars. Furthermore, we investigated the differential expression patterns of reactive oxygen species (ROS)-scavenging enzymes, heat shock proteins, and heat-stress transcription factors using qRT-PCR to confirm the differences in gene expression among the three varieties under heat stress. This study contributes to a better understanding of the wheat heat-stress response at the early growth stage and the varietal differences in heat tolerance.
format Online
Article
Text
id pubmed-9505106
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-95051062022-09-24 Comparison of Gene Expression Changes in Three Wheat Varieties with Different Susceptibilities to Heat Stress Using RNA-Seq Analysis Lee, Myoung Hui Kim, Kyeong-Min Sang, Wan-Gyu Kang, Chon-Sik Choi, Changhyun Int J Mol Sci Article Wheat is highly susceptible to heat stress, which significantly reduces grain yield. In this study, we used RNA-seq technology to analyze the transcript expression at three different time-points after heat treatment in three cultivars differing in their susceptibility to heat stress: Jopum, Keumkang, and Olgeuru. A total of 11,751, 8850, and 14,711; 10,959, 7946, and 14,205; and 22,895, 13,060, and 19,408 differentially-expressed genes (log2 fold-change > 1 and FDR (padj) < 0.05) were identified in Jopum, Keumkang, and Olgeuru in the control vs. 6-h, in the control vs. 12-h, and in the 6-h vs. 12-h heat treatment, respectively. Functional enrichment analysis showed that the biological processes for DEGs, such as the cellular response to heat and oxidative stress—and including the removal of superoxide radicals and the positive regulation of superoxide dismutase activity—were significantly enriched among the three comparisons in all three cultivars. Furthermore, we investigated the differential expression patterns of reactive oxygen species (ROS)-scavenging enzymes, heat shock proteins, and heat-stress transcription factors using qRT-PCR to confirm the differences in gene expression among the three varieties under heat stress. This study contributes to a better understanding of the wheat heat-stress response at the early growth stage and the varietal differences in heat tolerance. MDPI 2022-09-14 /pmc/articles/PMC9505106/ /pubmed/36142649 http://dx.doi.org/10.3390/ijms231810734 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Myoung Hui
Kim, Kyeong-Min
Sang, Wan-Gyu
Kang, Chon-Sik
Choi, Changhyun
Comparison of Gene Expression Changes in Three Wheat Varieties with Different Susceptibilities to Heat Stress Using RNA-Seq Analysis
title Comparison of Gene Expression Changes in Three Wheat Varieties with Different Susceptibilities to Heat Stress Using RNA-Seq Analysis
title_full Comparison of Gene Expression Changes in Three Wheat Varieties with Different Susceptibilities to Heat Stress Using RNA-Seq Analysis
title_fullStr Comparison of Gene Expression Changes in Three Wheat Varieties with Different Susceptibilities to Heat Stress Using RNA-Seq Analysis
title_full_unstemmed Comparison of Gene Expression Changes in Three Wheat Varieties with Different Susceptibilities to Heat Stress Using RNA-Seq Analysis
title_short Comparison of Gene Expression Changes in Three Wheat Varieties with Different Susceptibilities to Heat Stress Using RNA-Seq Analysis
title_sort comparison of gene expression changes in three wheat varieties with different susceptibilities to heat stress using rna-seq analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505106/
https://www.ncbi.nlm.nih.gov/pubmed/36142649
http://dx.doi.org/10.3390/ijms231810734
work_keys_str_mv AT leemyounghui comparisonofgeneexpressionchangesinthreewheatvarietieswithdifferentsusceptibilitiestoheatstressusingrnaseqanalysis
AT kimkyeongmin comparisonofgeneexpressionchangesinthreewheatvarietieswithdifferentsusceptibilitiestoheatstressusingrnaseqanalysis
AT sangwangyu comparisonofgeneexpressionchangesinthreewheatvarietieswithdifferentsusceptibilitiestoheatstressusingrnaseqanalysis
AT kangchonsik comparisonofgeneexpressionchangesinthreewheatvarietieswithdifferentsusceptibilitiestoheatstressusingrnaseqanalysis
AT choichanghyun comparisonofgeneexpressionchangesinthreewheatvarietieswithdifferentsusceptibilitiestoheatstressusingrnaseqanalysis