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Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion

Human noroviruses (HuNoVs) are the main cause of acute gastroenteritis causing more than 50,000 deaths per year. Recent evidence shows that the gut microbiota plays a key role in enteric virus infectivity. In this context, we tested whether microbiota depletion or microbiota replacement with that of...

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Autores principales: Santiso-Bellón, Cristina, Gozalbo-Rovira, Roberto, Buesa, Javier, Rubio-del-Campo, Antonio, Peña-Gil, Nazaret, Navarro-Lleó, Noemi, Cárcamo-Calvo, Roberto, Yebra, María J., Monedero, Vicente, Rodríguez-Díaz, Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505278/
https://www.ncbi.nlm.nih.gov/pubmed/36142552
http://dx.doi.org/10.3390/ijms231810643
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author Santiso-Bellón, Cristina
Gozalbo-Rovira, Roberto
Buesa, Javier
Rubio-del-Campo, Antonio
Peña-Gil, Nazaret
Navarro-Lleó, Noemi
Cárcamo-Calvo, Roberto
Yebra, María J.
Monedero, Vicente
Rodríguez-Díaz, Jesús
author_facet Santiso-Bellón, Cristina
Gozalbo-Rovira, Roberto
Buesa, Javier
Rubio-del-Campo, Antonio
Peña-Gil, Nazaret
Navarro-Lleó, Noemi
Cárcamo-Calvo, Roberto
Yebra, María J.
Monedero, Vicente
Rodríguez-Díaz, Jesús
author_sort Santiso-Bellón, Cristina
collection PubMed
description Human noroviruses (HuNoVs) are the main cause of acute gastroenteritis causing more than 50,000 deaths per year. Recent evidence shows that the gut microbiota plays a key role in enteric virus infectivity. In this context, we tested whether microbiota depletion or microbiota replacement with that of human individuals susceptible to HuNoVs infection could favor viral replication in mice. Four groups of mice (n = 5) were used, including a control group and three groups that were treated with antibiotics to eliminate the autochthonous intestinal microbiota. Two of the antibiotic-treated groups received fecal microbiota transplantation from a pool of feces from infants (age 1–3 months) or an auto-transplantation with mouse feces that obtained prior antibiotic treatment. The inoculation of the different mouse groups with a HuNoVs strain (GII.4 Sydney [P16] genotype) showed that the virus replicated more efficiently in animals only treated with antibiotics but not subject to microbiota transplantation. Viral replication in animals receiving fecal microbiota from newborn infants was intermediate, whereas virus excretion in feces from auto-transplanted mice was as low as in the control mice. The analysis of the fecal microbiota by 16S rDNA NGS showed deep variations in the composition in the different mice groups. Furthermore, differences were observed in the gene expression of relevant immunological mediators, such as IL4, CXCL15, IL13, TNFα and TLR2, at the small intestine. Our results suggest that microbiota depletion eliminates bacteria that restrict HuNoVs infectivity and that the mechanism(s) could involve immune mediators.
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spelling pubmed-95052782022-09-24 Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion Santiso-Bellón, Cristina Gozalbo-Rovira, Roberto Buesa, Javier Rubio-del-Campo, Antonio Peña-Gil, Nazaret Navarro-Lleó, Noemi Cárcamo-Calvo, Roberto Yebra, María J. Monedero, Vicente Rodríguez-Díaz, Jesús Int J Mol Sci Article Human noroviruses (HuNoVs) are the main cause of acute gastroenteritis causing more than 50,000 deaths per year. Recent evidence shows that the gut microbiota plays a key role in enteric virus infectivity. In this context, we tested whether microbiota depletion or microbiota replacement with that of human individuals susceptible to HuNoVs infection could favor viral replication in mice. Four groups of mice (n = 5) were used, including a control group and three groups that were treated with antibiotics to eliminate the autochthonous intestinal microbiota. Two of the antibiotic-treated groups received fecal microbiota transplantation from a pool of feces from infants (age 1–3 months) or an auto-transplantation with mouse feces that obtained prior antibiotic treatment. The inoculation of the different mouse groups with a HuNoVs strain (GII.4 Sydney [P16] genotype) showed that the virus replicated more efficiently in animals only treated with antibiotics but not subject to microbiota transplantation. Viral replication in animals receiving fecal microbiota from newborn infants was intermediate, whereas virus excretion in feces from auto-transplanted mice was as low as in the control mice. The analysis of the fecal microbiota by 16S rDNA NGS showed deep variations in the composition in the different mice groups. Furthermore, differences were observed in the gene expression of relevant immunological mediators, such as IL4, CXCL15, IL13, TNFα and TLR2, at the small intestine. Our results suggest that microbiota depletion eliminates bacteria that restrict HuNoVs infectivity and that the mechanism(s) could involve immune mediators. MDPI 2022-09-13 /pmc/articles/PMC9505278/ /pubmed/36142552 http://dx.doi.org/10.3390/ijms231810643 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Santiso-Bellón, Cristina
Gozalbo-Rovira, Roberto
Buesa, Javier
Rubio-del-Campo, Antonio
Peña-Gil, Nazaret
Navarro-Lleó, Noemi
Cárcamo-Calvo, Roberto
Yebra, María J.
Monedero, Vicente
Rodríguez-Díaz, Jesús
Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion
title Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion
title_full Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion
title_fullStr Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion
title_full_unstemmed Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion
title_short Replication of Human Norovirus in Mice after Antibiotic-Mediated Intestinal Bacteria Depletion
title_sort replication of human norovirus in mice after antibiotic-mediated intestinal bacteria depletion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505278/
https://www.ncbi.nlm.nih.gov/pubmed/36142552
http://dx.doi.org/10.3390/ijms231810643
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